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Efficient synthesis of a maghemite/gold hybrid nanoparticle system as a magnetic carrier for the transport of platinum-based metallotherapeutics.

Štarha P, Smola D, Tuček J, Trávníček Z - Int J Mol Sci (2015)

Bottom Line: HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy.In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold.The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

View Article: PubMed Central - PubMed

Affiliation: Regional Centre of Advanced Technologies and Materials, Department of Inorganic Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, Olomouc CZ-77146, Czech Republic. pavel.starha@upol.cz.

ABSTRACT
The preparation and thorough characterization of a hybrid magnetic carrier system for the possible transport of activated platinum-based anticancer drugs, as demonstrated for cisplatin (cis-[Pt(NH3)2Cl2], CDDP), are described. The final functionalized mag/Au-LA-CDDP* system consists of maghemite/gold nanoparticles (mag/Au) coated by lipoic acid (HLA; LA stands for deprotonated form of lipoic acid) and functionalized by activated cisplatin in the form of cis-[Pt(NH3)2(H2O)2]2+ (CDDP*). The relevant techniques (XPS, EDS, ICP-MS) proved the incorporation of the platinum-containing species on the surface of the studied hybrid system. HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy. In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold. The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

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The IR spectra of the mag/Au–HLA nanoparticles (green; the detail of the aliphatic C–H vibration region is given as an inset) and its comparison with the mag nanoparticles (mag; black) and free lipoic acid (HLA; red).
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ijms-16-02034-f007: The IR spectra of the mag/Au–HLA nanoparticles (green; the detail of the aliphatic C–H vibration region is given as an inset) and its comparison with the mag nanoparticles (mag; black) and free lipoic acid (HLA; red).

Mentions: The IR spectrum of mag/Au–HLA is depicted, together with the spectra of mag nanoparticles and free HLA, in Figure 7. Both the spectra of mag/Au–HLA and mag nanoparticles contained peak at ca. 540 cm−1, clearly assignable to the ν (Fe–O) vibration of maghemite [24,38]. In the spectrum of mag/Au–HLA, we also detected a series of peaks revealed at 1604 and 1387 cm−1, which may be associated with the C=O and C–O stretching vibrations, respectively, and the peaks at 2931 and 2974 cm−1 corresponding to the stretching vibrations of aliphatic C–H bonds. The peaks observed at ca. 920 and 629 cm−1 could be connected with the deformation O–H vibrations and stretching C–S vibrations, respectively. These results can support the presence of HLA in the discussed mag/Au–HLA nanoparticles, since their positions correlate well with the positions of the peaks in the spectrum of free lipoic acid [39,40].


Efficient synthesis of a maghemite/gold hybrid nanoparticle system as a magnetic carrier for the transport of platinum-based metallotherapeutics.

Štarha P, Smola D, Tuček J, Trávníček Z - Int J Mol Sci (2015)

The IR spectra of the mag/Au–HLA nanoparticles (green; the detail of the aliphatic C–H vibration region is given as an inset) and its comparison with the mag nanoparticles (mag; black) and free lipoic acid (HLA; red).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307347&req=5

ijms-16-02034-f007: The IR spectra of the mag/Au–HLA nanoparticles (green; the detail of the aliphatic C–H vibration region is given as an inset) and its comparison with the mag nanoparticles (mag; black) and free lipoic acid (HLA; red).
Mentions: The IR spectrum of mag/Au–HLA is depicted, together with the spectra of mag nanoparticles and free HLA, in Figure 7. Both the spectra of mag/Au–HLA and mag nanoparticles contained peak at ca. 540 cm−1, clearly assignable to the ν (Fe–O) vibration of maghemite [24,38]. In the spectrum of mag/Au–HLA, we also detected a series of peaks revealed at 1604 and 1387 cm−1, which may be associated with the C=O and C–O stretching vibrations, respectively, and the peaks at 2931 and 2974 cm−1 corresponding to the stretching vibrations of aliphatic C–H bonds. The peaks observed at ca. 920 and 629 cm−1 could be connected with the deformation O–H vibrations and stretching C–S vibrations, respectively. These results can support the presence of HLA in the discussed mag/Au–HLA nanoparticles, since their positions correlate well with the positions of the peaks in the spectrum of free lipoic acid [39,40].

Bottom Line: HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy.In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold.The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

View Article: PubMed Central - PubMed

Affiliation: Regional Centre of Advanced Technologies and Materials, Department of Inorganic Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, Olomouc CZ-77146, Czech Republic. pavel.starha@upol.cz.

ABSTRACT
The preparation and thorough characterization of a hybrid magnetic carrier system for the possible transport of activated platinum-based anticancer drugs, as demonstrated for cisplatin (cis-[Pt(NH3)2Cl2], CDDP), are described. The final functionalized mag/Au-LA-CDDP* system consists of maghemite/gold nanoparticles (mag/Au) coated by lipoic acid (HLA; LA stands for deprotonated form of lipoic acid) and functionalized by activated cisplatin in the form of cis-[Pt(NH3)2(H2O)2]2+ (CDDP*). The relevant techniques (XPS, EDS, ICP-MS) proved the incorporation of the platinum-containing species on the surface of the studied hybrid system. HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy. In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold. The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

Show MeSH