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Efficient synthesis of a maghemite/gold hybrid nanoparticle system as a magnetic carrier for the transport of platinum-based metallotherapeutics.

Štarha P, Smola D, Tuček J, Trávníček Z - Int J Mol Sci (2015)

Bottom Line: HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy.In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold.The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

View Article: PubMed Central - PubMed

Affiliation: Regional Centre of Advanced Technologies and Materials, Department of Inorganic Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, Olomouc CZ-77146, Czech Republic. pavel.starha@upol.cz.

ABSTRACT
The preparation and thorough characterization of a hybrid magnetic carrier system for the possible transport of activated platinum-based anticancer drugs, as demonstrated for cisplatin (cis-[Pt(NH3)2Cl2], CDDP), are described. The final functionalized mag/Au-LA-CDDP* system consists of maghemite/gold nanoparticles (mag/Au) coated by lipoic acid (HLA; LA stands for deprotonated form of lipoic acid) and functionalized by activated cisplatin in the form of cis-[Pt(NH3)2(H2O)2]2+ (CDDP*). The relevant techniques (XPS, EDS, ICP-MS) proved the incorporation of the platinum-containing species on the surface of the studied hybrid system. HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy. In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold. The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

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The results of the XPS spectroscopy of the mag/Au–LA–CDDP* nanosystems (red lines) and their comparison with mag/Au–HLA (black lines), given for the 0–750 eV region (top) with the details of the Pt4f/Au4f region at 67.5–90 eV (bottom left) and C1s region in the 280–295 eV range (bottom right).
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ijms-16-02034-f004: The results of the XPS spectroscopy of the mag/Au–LA–CDDP* nanosystems (red lines) and their comparison with mag/Au–HLA (black lines), given for the 0–750 eV region (top) with the details of the Pt4f/Au4f region at 67.5–90 eV (bottom left) and C1s region in the 280–295 eV range (bottom right).

Mentions: The XPS spectra were recorded for the final mag/Au–LA–CDDP* nanosystem and its synthetic precursor, mag/Au–HLA (Figure 4). As can be anticipated, the XPS spectra of both systems were similar (the data given below belong to mag/Au–LA–CDDP*). The photoelectron peaks assignable to the maghemite part of the composite were at 711.0 and 724.2 eV (Fe2p3/2 and Fe2p1/2) and 530.2 eV (O1s) [24,28]. The spectra also contained several gold photoelectron peaks, namely Au4p3/2 (353.8 eV), Au4p5/2 (333.4 eV), Au4f5/2 (87.0 eV) and Au4f7/2 (83.8 eV) [29,30,31]. Similar data were also reported for the gold nanoparticles [32] or gold seeds on the surface of the magnetite nanoparticles [27], which were covalently layered with the herein used lipoic acid. As for the lipoic acid itself, the peaks of C1s at 284.6 eV (aliphatic chain carbon atoms) and 288.4 eV (carboxylic group carbon atoms), as well as the S2p peak (163.0 eV) have to be considered as evidence of the lipoic acid incorporation into the studied nanoparticles [27,33].


Efficient synthesis of a maghemite/gold hybrid nanoparticle system as a magnetic carrier for the transport of platinum-based metallotherapeutics.

Štarha P, Smola D, Tuček J, Trávníček Z - Int J Mol Sci (2015)

The results of the XPS spectroscopy of the mag/Au–LA–CDDP* nanosystems (red lines) and their comparison with mag/Au–HLA (black lines), given for the 0–750 eV region (top) with the details of the Pt4f/Au4f region at 67.5–90 eV (bottom left) and C1s region in the 280–295 eV range (bottom right).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307347&req=5

ijms-16-02034-f004: The results of the XPS spectroscopy of the mag/Au–LA–CDDP* nanosystems (red lines) and their comparison with mag/Au–HLA (black lines), given for the 0–750 eV region (top) with the details of the Pt4f/Au4f region at 67.5–90 eV (bottom left) and C1s region in the 280–295 eV range (bottom right).
Mentions: The XPS spectra were recorded for the final mag/Au–LA–CDDP* nanosystem and its synthetic precursor, mag/Au–HLA (Figure 4). As can be anticipated, the XPS spectra of both systems were similar (the data given below belong to mag/Au–LA–CDDP*). The photoelectron peaks assignable to the maghemite part of the composite were at 711.0 and 724.2 eV (Fe2p3/2 and Fe2p1/2) and 530.2 eV (O1s) [24,28]. The spectra also contained several gold photoelectron peaks, namely Au4p3/2 (353.8 eV), Au4p5/2 (333.4 eV), Au4f5/2 (87.0 eV) and Au4f7/2 (83.8 eV) [29,30,31]. Similar data were also reported for the gold nanoparticles [32] or gold seeds on the surface of the magnetite nanoparticles [27], which were covalently layered with the herein used lipoic acid. As for the lipoic acid itself, the peaks of C1s at 284.6 eV (aliphatic chain carbon atoms) and 288.4 eV (carboxylic group carbon atoms), as well as the S2p peak (163.0 eV) have to be considered as evidence of the lipoic acid incorporation into the studied nanoparticles [27,33].

Bottom Line: HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy.In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold.The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

View Article: PubMed Central - PubMed

Affiliation: Regional Centre of Advanced Technologies and Materials, Department of Inorganic Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, Olomouc CZ-77146, Czech Republic. pavel.starha@upol.cz.

ABSTRACT
The preparation and thorough characterization of a hybrid magnetic carrier system for the possible transport of activated platinum-based anticancer drugs, as demonstrated for cisplatin (cis-[Pt(NH3)2Cl2], CDDP), are described. The final functionalized mag/Au-LA-CDDP* system consists of maghemite/gold nanoparticles (mag/Au) coated by lipoic acid (HLA; LA stands for deprotonated form of lipoic acid) and functionalized by activated cisplatin in the form of cis-[Pt(NH3)2(H2O)2]2+ (CDDP*). The relevant techniques (XPS, EDS, ICP-MS) proved the incorporation of the platinum-containing species on the surface of the studied hybrid system. HRTEM, TEM and SEM images showed the nanoparticles as spherical with an average size of 12 nm, while their superparamagnetic feature was proven by 57Fe Mössbauer spectroscopy. In the case of mag/Au, mag/Au-HLA and mag/Au-LA-CDDP*, weaker magnetic interactions among the Fe3+ centers of maghemite, as compared to maghemite nanoparticles (mag), were detected, which can be associated with the non-covalent coating of the maghemite surface by gold. The pH and time-dependent stability of the mag/Au-LA-CDDP* system in different media, represented by acetate (pH 5.0), phosphate (pH 7.0) and carbonate (pH 9.0) buffers and connected with the release of the platinum-containing species, showed the ability of CDDP* to be released from the functionalized nanosystem.

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