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Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

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Related in: MedlinePlus

Transcriptional analysis of DkNAC genes. Transcripts of DkNAC genes were measured by real-time PCR. Fruit were treated with 95% CO2 for one day at 20 °C in sealed container, while control fruit was sealed in a similar container without any treatment. Day 0 fruit values were set as 1. Error bars indicate standard error from three biological replicates.
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ijms-16-01894-f006: Transcriptional analysis of DkNAC genes. Transcripts of DkNAC genes were measured by real-time PCR. Fruit were treated with 95% CO2 for one day at 20 °C in sealed container, while control fruit was sealed in a similar container without any treatment. Day 0 fruit values were set as 1. Error bars indicate standard error from three biological replicates.

Mentions: The six NAC genes had different expression patterns. Transcripts of DkNAC1, DkNAC5 and DkNAC6 were transiently induced by the CO2 treatment, peaking after one day, and the expression of DkNAC3 peaked after two days. DkNAC5 was the most strongly up-regulated, with its mRNA increasing in abundance approximately 1200-fold following the treatment. Unlike DkNAC1/3/5/6, transcripts of DkNAC2 increased at four days (three days after treatment was stopped). Unlike the other DkNAC genes, the expression of DkNAC4 was decreased during CO2 treatment and then recovered by day 4 (Figure 6).


Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Transcriptional analysis of DkNAC genes. Transcripts of DkNAC genes were measured by real-time PCR. Fruit were treated with 95% CO2 for one day at 20 °C in sealed container, while control fruit was sealed in a similar container without any treatment. Day 0 fruit values were set as 1. Error bars indicate standard error from three biological replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307340&req=5

ijms-16-01894-f006: Transcriptional analysis of DkNAC genes. Transcripts of DkNAC genes were measured by real-time PCR. Fruit were treated with 95% CO2 for one day at 20 °C in sealed container, while control fruit was sealed in a similar container without any treatment. Day 0 fruit values were set as 1. Error bars indicate standard error from three biological replicates.
Mentions: The six NAC genes had different expression patterns. Transcripts of DkNAC1, DkNAC5 and DkNAC6 were transiently induced by the CO2 treatment, peaking after one day, and the expression of DkNAC3 peaked after two days. DkNAC5 was the most strongly up-regulated, with its mRNA increasing in abundance approximately 1200-fold following the treatment. Unlike DkNAC1/3/5/6, transcripts of DkNAC2 increased at four days (three days after treatment was stopped). Unlike the other DkNAC genes, the expression of DkNAC4 was decreased during CO2 treatment and then recovered by day 4 (Figure 6).

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

Show MeSH
Related in: MedlinePlus