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Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

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Phylogenetic tree of NAC genes. Persimmon DkNAC genes are highlighted in red. The amino acid sequences of the Arabidopsis ERF family were obtained from TAIR. The phylogenetic tree was constructed with figtree (version 3.1).
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ijms-16-01894-f005: Phylogenetic tree of NAC genes. Persimmon DkNAC genes are highlighted in red. The amino acid sequences of the Arabidopsis ERF family were obtained from TAIR. The phylogenetic tree was constructed with figtree (version 3.1).

Mentions: To examine the phylogenetic relationship between the NAC proteins in persimmon and Arabidopsis, a phylogenetic tree was constructed based on their translated amino acid sequences. As shown in Figure 5, DkNAC1 and DkNAC6 were homologous with, and closely related to ANAC102, which is involved in the viability of Arabidopsis seeds following low-oxygen treatment [19]; DkNAC2 and DkNAC3 share the same branch, and are closely related to ANAC029, which was reported to play an important role in leaf senescence in Arabidopsis [28]; DkNAC4 was clustered with ANAC013, which has been reported to be involved in mitochondrial retrograde regulation of the oxidative stress response in Arabidopsis [29]; DkNAC5 was similar to ANAC104, which was identified as part of the leaf senescence transcriptome [30]. Collectively, these data suggested that DkNAC1-6 may exhibit diverse functions.


Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Phylogenetic tree of NAC genes. Persimmon DkNAC genes are highlighted in red. The amino acid sequences of the Arabidopsis ERF family were obtained from TAIR. The phylogenetic tree was constructed with figtree (version 3.1).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307340&req=5

ijms-16-01894-f005: Phylogenetic tree of NAC genes. Persimmon DkNAC genes are highlighted in red. The amino acid sequences of the Arabidopsis ERF family were obtained from TAIR. The phylogenetic tree was constructed with figtree (version 3.1).
Mentions: To examine the phylogenetic relationship between the NAC proteins in persimmon and Arabidopsis, a phylogenetic tree was constructed based on their translated amino acid sequences. As shown in Figure 5, DkNAC1 and DkNAC6 were homologous with, and closely related to ANAC102, which is involved in the viability of Arabidopsis seeds following low-oxygen treatment [19]; DkNAC2 and DkNAC3 share the same branch, and are closely related to ANAC029, which was reported to play an important role in leaf senescence in Arabidopsis [28]; DkNAC4 was clustered with ANAC013, which has been reported to be involved in mitochondrial retrograde regulation of the oxidative stress response in Arabidopsis [29]; DkNAC5 was similar to ANAC104, which was identified as part of the leaf senescence transcriptome [30]. Collectively, these data suggested that DkNAC1-6 may exhibit diverse functions.

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

Show MeSH
Related in: MedlinePlus