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Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

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Amino acid sequence alignment of the DkNAC proteins with Arabidopsis and rice NAC proteins. DkNAC proteins were aligned with Arabidopsis ANAC019 (At1g52890.1), ANAC042 (At2g43000.1), ANAC043 (At2g46770.1), ANAC102 (AT5G63790.1) and rice ONAC022 (AK107090). Identical and similar amino acids are indicated by black and grey shading, respectively. Gaps were introduced to optimize alignment. The five highly conserved amino acid motifs (A–E) are indicated by black lines.
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ijms-16-01894-f004: Amino acid sequence alignment of the DkNAC proteins with Arabidopsis and rice NAC proteins. DkNAC proteins were aligned with Arabidopsis ANAC019 (At1g52890.1), ANAC042 (At2g43000.1), ANAC043 (At2g46770.1), ANAC102 (AT5G63790.1) and rice ONAC022 (AK107090). Identical and similar amino acids are indicated by black and grey shading, respectively. Gaps were introduced to optimize alignment. The five highly conserved amino acid motifs (A–E) are indicated by black lines.

Mentions: Alignment analysis with the deduced proteins encoded by the DkNAC genes showed that they contained the NAC conserved domain in their N-terminal regions, which was divided into five subdomains (A–E) [27] (Figure 4). However, the C-terminal regions showed less similarity between different members of the NAC family (Figure 4).


Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Amino acid sequence alignment of the DkNAC proteins with Arabidopsis and rice NAC proteins. DkNAC proteins were aligned with Arabidopsis ANAC019 (At1g52890.1), ANAC042 (At2g43000.1), ANAC043 (At2g46770.1), ANAC102 (AT5G63790.1) and rice ONAC022 (AK107090). Identical and similar amino acids are indicated by black and grey shading, respectively. Gaps were introduced to optimize alignment. The five highly conserved amino acid motifs (A–E) are indicated by black lines.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307340&req=5

ijms-16-01894-f004: Amino acid sequence alignment of the DkNAC proteins with Arabidopsis and rice NAC proteins. DkNAC proteins were aligned with Arabidopsis ANAC019 (At1g52890.1), ANAC042 (At2g43000.1), ANAC043 (At2g46770.1), ANAC102 (AT5G63790.1) and rice ONAC022 (AK107090). Identical and similar amino acids are indicated by black and grey shading, respectively. Gaps were introduced to optimize alignment. The five highly conserved amino acid motifs (A–E) are indicated by black lines.
Mentions: Alignment analysis with the deduced proteins encoded by the DkNAC genes showed that they contained the NAC conserved domain in their N-terminal regions, which was divided into five subdomains (A–E) [27] (Figure 4). However, the C-terminal regions showed less similarity between different members of the NAC family (Figure 4).

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

Show MeSH
Related in: MedlinePlus