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Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

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Comparison of tannin printing of control and CO2 treated “Mopan” fruit at 2 days in storage.
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ijms-16-01894-f002: Comparison of tannin printing of control and CO2 treated “Mopan” fruit at 2 days in storage.

Mentions: The soluble tannin content remained almost constant during storage in the control fruit, whereas in contrast, the CO2 treatment caused a rapid decrease in the concentration of soluble tannin from 0.917% at day 0% to 0.229% at day 1 (Figure 1). Soluble tannins content was measured both by the Folin phenol method, and also visualized by tannin printing, using filter paper soaked with 5% FeCl2. The extent, and location, of soluble tannin content was revealed by the tissue printing, and was substantially lower in CO2 treated fruit, compared with control fruit (Figure 2).


Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.

Min T, Wang MM, Wang H, Liu X, Fang F, Grierson D, Yin XR, Chen KS - Int J Mol Sci (2015)

Comparison of tannin printing of control and CO2 treated “Mopan” fruit at 2 days in storage.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307340&req=5

ijms-16-01894-f002: Comparison of tannin printing of control and CO2 treated “Mopan” fruit at 2 days in storage.
Mentions: The soluble tannin content remained almost constant during storage in the control fruit, whereas in contrast, the CO2 treatment caused a rapid decrease in the concentration of soluble tannin from 0.917% at day 0% to 0.229% at day 1 (Figure 1). Soluble tannins content was measured both by the Folin phenol method, and also visualized by tannin printing, using filter paper soaked with 5% FeCl2. The extent, and location, of soluble tannin content was revealed by the tissue printing, and was substantially lower in CO2 treated fruit, compared with control fruit (Figure 2).

Bottom Line: In model plants, NAC genes have been identified as being responsive to low oxygen.In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin.Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.

ABSTRACT
NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.

Show MeSH
Related in: MedlinePlus