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Specificity protein 1 regulates gene expression related to fatty acid metabolism in goat mammary epithelial cells.

Zhu J, Sun Y, Luo J, Wu M, Li J, Cao Y - Int J Mol Sci (2015)

Bottom Line: There were no significant expression level differences between the mammary gland tissues collected at lactation and dry-off period.These results were further enhanced by the silencing of SP1.These findings suggest that SP1 may play an important role in fatty acid metabolism.

View Article: PubMed Central - PubMed

Affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. zhujiang4656@hotmail.com.

ABSTRACT
Specificity protein 1 (SP1) is a ubiquitous transcription factor that plays an important role in controlling gene expression. Although important in mediating the function of various hormones, the role of SP1 in regulating milk fat formation remains unknown. To investigate the sequence and expression information, as well as its role in modulating lipid metabolism, we cloned SP1 gene from mammary gland of Xinong Saanen dairy goat. The full-length cDNA of the SP1 gene is 4376 bp including 103 bp of 5'UTR, 2358 bp of ORF (HM_236311) and 1915 bp of 3'UTR, which is predicted to encode a 786 amino acids polypeptide. Phylogenetic tree analysis showed that goat SP1 has the closest relationship with sheep, followed by bovines (bos taurus, odobenus and ceratotherium), pig, primates (pongo, gorilla, macaca and papio) and murine (rattus and mus), while the furthest relationship was with canis and otolemur. Expression was predominant in the lungs, small intestine, muscle, spleen, mammary gland and subcutaneous fat. There were no significant expression level differences between the mammary gland tissues collected at lactation and dry-off period. Overexpression of SP1 in goat mammary epithelial cells (GMECs) led to higher mRNA expression level of peroxisome proliferator-activated receptor-γ (PPARγ) and lower liver X receptor α (LXRα) mRNA level, both of which were crucial in regulating fatty acid metabolism, and correspondingly altered the expression of their downstream genes in GMECs. These results were further enhanced by the silencing of SP1. These findings suggest that SP1 may play an important role in fatty acid metabolism.

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Phylogenetic tree based on SP1 gene sequences of 31 representative animals made with MEGA 5 software (S. Kumar, Tempe, AZ, USA) using Neighbor-Joining (NJ) method.
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ijms-16-01806-f002: Phylogenetic tree based on SP1 gene sequences of 31 representative animals made with MEGA 5 software (S. Kumar, Tempe, AZ, USA) using Neighbor-Joining (NJ) method.

Mentions: To investigate potential evolutionary processes of the SP1 gene among various species, a neighbor-joining phylogenetic tree was constructed, based on sequences of 31 representative animals. The result shows that goat SP1 has the closest relationship with sheep, followed by bovines (including bos taurus, odobenus and cerato therium), pig, primates (pongo, gorilla, macaca and papio) and murine (rattus and mus). Canis and otolemur show the greatest distance from capra hircus (Figure 2).


Specificity protein 1 regulates gene expression related to fatty acid metabolism in goat mammary epithelial cells.

Zhu J, Sun Y, Luo J, Wu M, Li J, Cao Y - Int J Mol Sci (2015)

Phylogenetic tree based on SP1 gene sequences of 31 representative animals made with MEGA 5 software (S. Kumar, Tempe, AZ, USA) using Neighbor-Joining (NJ) method.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307335&req=5

ijms-16-01806-f002: Phylogenetic tree based on SP1 gene sequences of 31 representative animals made with MEGA 5 software (S. Kumar, Tempe, AZ, USA) using Neighbor-Joining (NJ) method.
Mentions: To investigate potential evolutionary processes of the SP1 gene among various species, a neighbor-joining phylogenetic tree was constructed, based on sequences of 31 representative animals. The result shows that goat SP1 has the closest relationship with sheep, followed by bovines (including bos taurus, odobenus and cerato therium), pig, primates (pongo, gorilla, macaca and papio) and murine (rattus and mus). Canis and otolemur show the greatest distance from capra hircus (Figure 2).

Bottom Line: There were no significant expression level differences between the mammary gland tissues collected at lactation and dry-off period.These results were further enhanced by the silencing of SP1.These findings suggest that SP1 may play an important role in fatty acid metabolism.

View Article: PubMed Central - PubMed

Affiliation: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. zhujiang4656@hotmail.com.

ABSTRACT
Specificity protein 1 (SP1) is a ubiquitous transcription factor that plays an important role in controlling gene expression. Although important in mediating the function of various hormones, the role of SP1 in regulating milk fat formation remains unknown. To investigate the sequence and expression information, as well as its role in modulating lipid metabolism, we cloned SP1 gene from mammary gland of Xinong Saanen dairy goat. The full-length cDNA of the SP1 gene is 4376 bp including 103 bp of 5'UTR, 2358 bp of ORF (HM_236311) and 1915 bp of 3'UTR, which is predicted to encode a 786 amino acids polypeptide. Phylogenetic tree analysis showed that goat SP1 has the closest relationship with sheep, followed by bovines (bos taurus, odobenus and ceratotherium), pig, primates (pongo, gorilla, macaca and papio) and murine (rattus and mus), while the furthest relationship was with canis and otolemur. Expression was predominant in the lungs, small intestine, muscle, spleen, mammary gland and subcutaneous fat. There were no significant expression level differences between the mammary gland tissues collected at lactation and dry-off period. Overexpression of SP1 in goat mammary epithelial cells (GMECs) led to higher mRNA expression level of peroxisome proliferator-activated receptor-γ (PPARγ) and lower liver X receptor α (LXRα) mRNA level, both of which were crucial in regulating fatty acid metabolism, and correspondingly altered the expression of their downstream genes in GMECs. These results were further enhanced by the silencing of SP1. These findings suggest that SP1 may play an important role in fatty acid metabolism.

Show MeSH