Limits...
Apigenin-7-glycoside prevents LPS-induced acute lung injury via downregulation of oxidative enzyme expression and protein activation through inhibition of MAPK phosphorylation.

Li KC, Ho YL, Hsieh WT, Huang SS, Chang YS, Huang GJ - Int J Mol Sci (2015)

Bottom Line: Additionally, inflammation, which can decrease the activities of antioxidative enzymes (AOEs) is also observed in these studies.At the same time, flavonoids are reported to promote the activities of heme oxygenase-1 (HO-1) decreased by LPS.Meanwhile, our data revealed that AP7Glu not only did significantly inhibit the LPS-enhanced inflammatory activity in lung, but also exhibited anti-inflammatory effect through the MAPK and inhibitor NF-κB (IκB) pathways.

View Article: PubMed Central - PubMed

Affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan. u9852852@cmu.edu.tw.

ABSTRACT
Apigenin-7-glycoside (AP7Glu) with multiple biological activities is a flavonoid that is currently prescribed to treat inflammatory diseases such as upper respiratory infections. Recently, several studies have shown that its anti-inflammatory activities have been strongly linked to the inhibition of secretion of pro-inflammatory proteins, such as inducible nitric oxide synthase (iNOs) and cyclooxygenase-2 (COX-2) induced through phosphorylation nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPK) pathways. Additionally, inflammation, which can decrease the activities of antioxidative enzymes (AOEs) is also observed in these studies. At the same time, flavonoids are reported to promote the activities of heme oxygenase-1 (HO-1) decreased by LPS. The purpose of this study was to assess these theories in a series of experiments on the suppressive effects of AP7Glu based on LPS-induced nitric oxide production in RAW264.7 macrophages in vitro and acute lung injury in mice in vivo. After six hours of lipopolysaccharide (LPS) stimulation, pulmonary pathological, myeloperoxidase (MPO) activity, total polymorphonuclear leukocytes (PMN) cells, cytokines in bronchoalveolar lavage fluid (BALF) and AOEs, are all affected and changed. Meanwhile, our data revealed that AP7Glu not only did significantly inhibit the LPS-enhanced inflammatory activity in lung, but also exhibited anti-inflammatory effect through the MAPK and inhibitor NF-κB (IκB) pathways.

Show MeSH

Related in: MedlinePlus

AP7Glu reduced cellular counts (A); and total protein (B) in BALF. Six hours after LPS injection with or without AP7Glu pretreatments, mice were sacrificed and their lungs were lavaged. Cells in the BALF were collected and cytospin preparations were made. Total cells, and total proteins in BALF were analyzed. Each value represents as mean ± SD of 6 mice. ### compared with sample of control group. (One-way ANOVA followed by Scheffe’s multiple range test). ** p < 0.01, and *** p < 0.001, were compared with LPS-alone group.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4307331&req=5

ijms-16-01736-f004: AP7Glu reduced cellular counts (A); and total protein (B) in BALF. Six hours after LPS injection with or without AP7Glu pretreatments, mice were sacrificed and their lungs were lavaged. Cells in the BALF were collected and cytospin preparations were made. Total cells, and total proteins in BALF were analyzed. Each value represents as mean ± SD of 6 mice. ### compared with sample of control group. (One-way ANOVA followed by Scheffe’s multiple range test). ** p < 0.01, and *** p < 0.001, were compared with LPS-alone group.

Mentions: To further identify the anti-inflammatory property of AP7Glu, an important feature is calculating the cellular counts and proteins of PMNs infiltration into the lungs (Figure 4A,B). We evaluated the alterations in leukocyte infiltration occurring in the lungs of LPS-administered mice with or without AP7Glu pretreatment. The number of infiltrating leukocytes was counted by cytometry, and total protein was calculated by Bradford assay on BALF.


Apigenin-7-glycoside prevents LPS-induced acute lung injury via downregulation of oxidative enzyme expression and protein activation through inhibition of MAPK phosphorylation.

Li KC, Ho YL, Hsieh WT, Huang SS, Chang YS, Huang GJ - Int J Mol Sci (2015)

AP7Glu reduced cellular counts (A); and total protein (B) in BALF. Six hours after LPS injection with or without AP7Glu pretreatments, mice were sacrificed and their lungs were lavaged. Cells in the BALF were collected and cytospin preparations were made. Total cells, and total proteins in BALF were analyzed. Each value represents as mean ± SD of 6 mice. ### compared with sample of control group. (One-way ANOVA followed by Scheffe’s multiple range test). ** p < 0.01, and *** p < 0.001, were compared with LPS-alone group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307331&req=5

ijms-16-01736-f004: AP7Glu reduced cellular counts (A); and total protein (B) in BALF. Six hours after LPS injection with or without AP7Glu pretreatments, mice were sacrificed and their lungs were lavaged. Cells in the BALF were collected and cytospin preparations were made. Total cells, and total proteins in BALF were analyzed. Each value represents as mean ± SD of 6 mice. ### compared with sample of control group. (One-way ANOVA followed by Scheffe’s multiple range test). ** p < 0.01, and *** p < 0.001, were compared with LPS-alone group.
Mentions: To further identify the anti-inflammatory property of AP7Glu, an important feature is calculating the cellular counts and proteins of PMNs infiltration into the lungs (Figure 4A,B). We evaluated the alterations in leukocyte infiltration occurring in the lungs of LPS-administered mice with or without AP7Glu pretreatment. The number of infiltrating leukocytes was counted by cytometry, and total protein was calculated by Bradford assay on BALF.

Bottom Line: Additionally, inflammation, which can decrease the activities of antioxidative enzymes (AOEs) is also observed in these studies.At the same time, flavonoids are reported to promote the activities of heme oxygenase-1 (HO-1) decreased by LPS.Meanwhile, our data revealed that AP7Glu not only did significantly inhibit the LPS-enhanced inflammatory activity in lung, but also exhibited anti-inflammatory effect through the MAPK and inhibitor NF-κB (IκB) pathways.

View Article: PubMed Central - PubMed

Affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung 404, Taiwan. u9852852@cmu.edu.tw.

ABSTRACT
Apigenin-7-glycoside (AP7Glu) with multiple biological activities is a flavonoid that is currently prescribed to treat inflammatory diseases such as upper respiratory infections. Recently, several studies have shown that its anti-inflammatory activities have been strongly linked to the inhibition of secretion of pro-inflammatory proteins, such as inducible nitric oxide synthase (iNOs) and cyclooxygenase-2 (COX-2) induced through phosphorylation nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPK) pathways. Additionally, inflammation, which can decrease the activities of antioxidative enzymes (AOEs) is also observed in these studies. At the same time, flavonoids are reported to promote the activities of heme oxygenase-1 (HO-1) decreased by LPS. The purpose of this study was to assess these theories in a series of experiments on the suppressive effects of AP7Glu based on LPS-induced nitric oxide production in RAW264.7 macrophages in vitro and acute lung injury in mice in vivo. After six hours of lipopolysaccharide (LPS) stimulation, pulmonary pathological, myeloperoxidase (MPO) activity, total polymorphonuclear leukocytes (PMN) cells, cytokines in bronchoalveolar lavage fluid (BALF) and AOEs, are all affected and changed. Meanwhile, our data revealed that AP7Glu not only did significantly inhibit the LPS-enhanced inflammatory activity in lung, but also exhibited anti-inflammatory effect through the MAPK and inhibitor NF-κB (IκB) pathways.

Show MeSH
Related in: MedlinePlus