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The cluster [Re6Se8I6]3- induces low hemolysis of human erythrocytes in vitro: protective effect of albumin.

Rojas-Mancilla E, Oyarce A, Verdugo V, Zheng Z, Ramírez-Tagle R - Int J Mol Sci (2015)

Bottom Line: Exposition to therapeutic doses of the cluster did not induce acute hemolysis.Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels.In conclusion, the cluster Re6Se8I63- could be intravenously administered in animals at therapeutic doses for in vivo studies.

View Article: PubMed Central - PubMed

Affiliation: Universidad Bernardo O' Higgins, Departamento de Ciencias Químicas y Biológicas, General Gana 1780, Santiago 8370854, Chile. eseba21@gmail.com.

ABSTRACT
The cluster Re6Se8I63- has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25-150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re6Se8I63- is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re6Se8I63- could be intravenously administered in animals at therapeutic doses for in vivo studies.

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Acute exposition to increasing doses of the cluster did not induce hemolysis in vitro. The percentage of hemolysis after 1 h of incubation is shown. Erythrocytes suspended in distilled water were considered 100% hemolyzed. High doses of the cluster did not increase hemolysis in vitro in the presence or absence of albumin, compared to controls.
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ijms-16-01728-f001: Acute exposition to increasing doses of the cluster did not induce hemolysis in vitro. The percentage of hemolysis after 1 h of incubation is shown. Erythrocytes suspended in distilled water were considered 100% hemolyzed. High doses of the cluster did not increase hemolysis in vitro in the presence or absence of albumin, compared to controls.

Mentions: Acute exposition of human erythrocytes to therapeutic doses of the cluster did not induce hemolysis in vitro (Figure 1). Additionally, acute exposition to high doses of the cluster induced alterations in the morphology of erythrocytes (Figure 2A); the normal size of erythrocytes ranged between 7 and 8 µm. Exposition to 100 and 150 µM of the cluster leads to a significant reduction in cell size (6.1 and 5.6 µm, respectively) compared to controls (7.3 µm, Figure 2B). Erythrocytes exposed to 100–150 µM of the cluster showed echinocytosis and crenation. Both morphological alterations and reduction in size were prevented by co-incubation with albumin (Figure 2). Similarly, very low hemolysis was observed after 24 h of incubation at 37 °C and under agitation (Figure 3). Furthermore, a reduction in the size of the erythrocytes was observed in all experimental conditions, with an average size of 6.5–7.0 µm (Figure 4).


The cluster [Re6Se8I6]3- induces low hemolysis of human erythrocytes in vitro: protective effect of albumin.

Rojas-Mancilla E, Oyarce A, Verdugo V, Zheng Z, Ramírez-Tagle R - Int J Mol Sci (2015)

Acute exposition to increasing doses of the cluster did not induce hemolysis in vitro. The percentage of hemolysis after 1 h of incubation is shown. Erythrocytes suspended in distilled water were considered 100% hemolyzed. High doses of the cluster did not increase hemolysis in vitro in the presence or absence of albumin, compared to controls.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307330&req=5

ijms-16-01728-f001: Acute exposition to increasing doses of the cluster did not induce hemolysis in vitro. The percentage of hemolysis after 1 h of incubation is shown. Erythrocytes suspended in distilled water were considered 100% hemolyzed. High doses of the cluster did not increase hemolysis in vitro in the presence or absence of albumin, compared to controls.
Mentions: Acute exposition of human erythrocytes to therapeutic doses of the cluster did not induce hemolysis in vitro (Figure 1). Additionally, acute exposition to high doses of the cluster induced alterations in the morphology of erythrocytes (Figure 2A); the normal size of erythrocytes ranged between 7 and 8 µm. Exposition to 100 and 150 µM of the cluster leads to a significant reduction in cell size (6.1 and 5.6 µm, respectively) compared to controls (7.3 µm, Figure 2B). Erythrocytes exposed to 100–150 µM of the cluster showed echinocytosis and crenation. Both morphological alterations and reduction in size were prevented by co-incubation with albumin (Figure 2). Similarly, very low hemolysis was observed after 24 h of incubation at 37 °C and under agitation (Figure 3). Furthermore, a reduction in the size of the erythrocytes was observed in all experimental conditions, with an average size of 6.5–7.0 µm (Figure 4).

Bottom Line: Exposition to therapeutic doses of the cluster did not induce acute hemolysis.Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels.In conclusion, the cluster Re6Se8I63- could be intravenously administered in animals at therapeutic doses for in vivo studies.

View Article: PubMed Central - PubMed

Affiliation: Universidad Bernardo O' Higgins, Departamento de Ciencias Químicas y Biológicas, General Gana 1780, Santiago 8370854, Chile. eseba21@gmail.com.

ABSTRACT
The cluster Re6Se8I63- has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25-150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re6Se8I63- is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re6Se8I63- could be intravenously administered in animals at therapeutic doses for in vivo studies.

Show MeSH
Related in: MedlinePlus