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Activation of the ubiquitin proteasome pathway by silk fibroin modified chitosan nanoparticles in hepatic cancer cells.

Yang MH, Chung TW, Lu YS, Chen YL, Tsai WC, Jong SB, Yuan SS, Liao PC, Lin PC, Tyan YC - Int J Mol Sci (2015)

Bottom Line: Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells.The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV.The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. myang@mail.kmuh.org.tw.

ABSTRACT
Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation.

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Lactate dehydrogenase (LDH) test of SF-CSNP and CSNP effects on CCL-13 and HepG2 cells (n = 6, mean ± standard error, t-test, p < 0.05).
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ijms-16-01657-f002: Lactate dehydrogenase (LDH) test of SF-CSNP and CSNP effects on CCL-13 and HepG2 cells (n = 6, mean ± standard error, t-test, p < 0.05).

Mentions: As shown in Figure 2, the LDH concentrations were decreased and observed between the groups treated with different concentration of CSNPs, SF-CSNPs and control (p < 0.05, n = 6). Compared with the control, the BrdU was upregulated in the groups treated with CSNPs and SF-CSNPs with significant increase (Figure 3, p < 0.05, n = 6). Those results indicate that no significant cytotoxicity was observed in the CSNPs and SF-CSNPs groups; and in addition, the CSNPs and SF-CSNPs showed improved cell growth and proliferation. Unlike metal or metal oxide nanoparticles, the CSNPs and SF-CSNPs were non-toxic. The cell growth and survival rate were increased with the higher concentration of CSNPs and SF-CSNPs. As shown in Figure 2 and Figure 3, the biocompatibility of SF-CSNPs was better than CSNPs; and the SF-CSNPs obviously enhanced HepG2 cell growth in a dose dependent manner.


Activation of the ubiquitin proteasome pathway by silk fibroin modified chitosan nanoparticles in hepatic cancer cells.

Yang MH, Chung TW, Lu YS, Chen YL, Tsai WC, Jong SB, Yuan SS, Liao PC, Lin PC, Tyan YC - Int J Mol Sci (2015)

Lactate dehydrogenase (LDH) test of SF-CSNP and CSNP effects on CCL-13 and HepG2 cells (n = 6, mean ± standard error, t-test, p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307326&req=5

ijms-16-01657-f002: Lactate dehydrogenase (LDH) test of SF-CSNP and CSNP effects on CCL-13 and HepG2 cells (n = 6, mean ± standard error, t-test, p < 0.05).
Mentions: As shown in Figure 2, the LDH concentrations were decreased and observed between the groups treated with different concentration of CSNPs, SF-CSNPs and control (p < 0.05, n = 6). Compared with the control, the BrdU was upregulated in the groups treated with CSNPs and SF-CSNPs with significant increase (Figure 3, p < 0.05, n = 6). Those results indicate that no significant cytotoxicity was observed in the CSNPs and SF-CSNPs groups; and in addition, the CSNPs and SF-CSNPs showed improved cell growth and proliferation. Unlike metal or metal oxide nanoparticles, the CSNPs and SF-CSNPs were non-toxic. The cell growth and survival rate were increased with the higher concentration of CSNPs and SF-CSNPs. As shown in Figure 2 and Figure 3, the biocompatibility of SF-CSNPs was better than CSNPs; and the SF-CSNPs obviously enhanced HepG2 cell growth in a dose dependent manner.

Bottom Line: Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells.The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV.The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. myang@mail.kmuh.org.tw.

ABSTRACT
Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation.

Show MeSH
Related in: MedlinePlus