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Protection of retina by mini-αA in NaIO3-induced retinal pigment epithelium degeneration mice.

Zhang J, Zhao X, Cai Y, Li Y, Yu X, Lu L - Int J Mol Sci (2015)

Bottom Line: Further investigation into how mini-αA provided protection against NaIO3-induced RPE cell apoptosis showed that mini-αA reduced NaIO3-induced RPE cell apoptosis and autophagy.In addition, unfolded protein response was also involved in the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. mini-αA can antagonize RPE cell apoptosis induced by NaIO3.A possible mechanism is by inhibition of apoptosis by repressing autophagy and endoplasmic reticulum stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. zhjinglin@126.com.

ABSTRACT

Background: Studies have shown that mini-αA can protect retinal pigment epithelium (RPE) cells from apoptosis. However, no in vivo study concerning the anti-apoptotic function of mini-αA has been conducted yet.

Methods: MTT assay, HE staining and TUNEL assay were used to assess levels of cells, and an animal model was established to examine the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. Western blot analysis and RT-qPCR were performed to explore the possible mechanism of mini-αA's protective function against NaIO3-induced RPE cell apoptosis.

Results: RESULTS from in vivo and animal experiments showed that mini-αA antagonized NaIO3-induced RPE cell apoptosis. Further investigation into how mini-αA provided protection against NaIO3-induced RPE cell apoptosis showed that mini-αA reduced NaIO3-induced RPE cell apoptosis and autophagy. In addition, unfolded protein response was also involved in the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis.

Conclusions: mini-αA can antagonize RPE cell apoptosis induced by NaIO3. A possible mechanism is by inhibition of apoptosis by repressing autophagy and endoplasmic reticulum stress.

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Western blotting was performed to detect caspase and 3LC3- I/II protein expression levels of each subgroup. (A) Caspase expression levels of each subgroup and quantification of the band intensity of caspase from there independent experiments; (B) 3LC3- I/II protein expression levels in each group and quantification of the band intensity from there independent experiments. GAPDH was used as the internal control. CTL: normal ARPE-19 cells; NaIO3: cells induced with NaIO3; NaIO3 + mini-αA: cells treated first with mini-αA and then with NaIO3. * p < 0.05 vs. Control; # p < 0.05 vs. NaIO3.
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ijms-16-01644-f004: Western blotting was performed to detect caspase and 3LC3- I/II protein expression levels of each subgroup. (A) Caspase expression levels of each subgroup and quantification of the band intensity of caspase from there independent experiments; (B) 3LC3- I/II protein expression levels in each group and quantification of the band intensity from there independent experiments. GAPDH was used as the internal control. CTL: normal ARPE-19 cells; NaIO3: cells induced with NaIO3; NaIO3 + mini-αA: cells treated first with mini-αA and then with NaIO3. * p < 0.05 vs. Control; # p < 0.05 vs. NaIO3.

Mentions: To explore the mechanism of mini-αA’s protective function against NaIO3-induced retinal degeneration, ARPE-19 cells were induced by NaIO3 at 2.5, 3 and 3.5 mM. Meanwhile, three subgroups treated with NaIO3 at 3.5 mM were set up, and were treated with mini-αA at concentrations of 10, 15 or 20 μM. Western blot analysis revealed that the expression of active-Caspase3 in cells treated with NaIO3 at 3.5 mM was much higher than that in normal cells, and the expression in cells treated with 3.5 mM NaIO3 and 20 μmM mini-αA decreased dramatically (Figure 4A).


Protection of retina by mini-αA in NaIO3-induced retinal pigment epithelium degeneration mice.

Zhang J, Zhao X, Cai Y, Li Y, Yu X, Lu L - Int J Mol Sci (2015)

Western blotting was performed to detect caspase and 3LC3- I/II protein expression levels of each subgroup. (A) Caspase expression levels of each subgroup and quantification of the band intensity of caspase from there independent experiments; (B) 3LC3- I/II protein expression levels in each group and quantification of the band intensity from there independent experiments. GAPDH was used as the internal control. CTL: normal ARPE-19 cells; NaIO3: cells induced with NaIO3; NaIO3 + mini-αA: cells treated first with mini-αA and then with NaIO3. * p < 0.05 vs. Control; # p < 0.05 vs. NaIO3.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4307325&req=5

ijms-16-01644-f004: Western blotting was performed to detect caspase and 3LC3- I/II protein expression levels of each subgroup. (A) Caspase expression levels of each subgroup and quantification of the band intensity of caspase from there independent experiments; (B) 3LC3- I/II protein expression levels in each group and quantification of the band intensity from there independent experiments. GAPDH was used as the internal control. CTL: normal ARPE-19 cells; NaIO3: cells induced with NaIO3; NaIO3 + mini-αA: cells treated first with mini-αA and then with NaIO3. * p < 0.05 vs. Control; # p < 0.05 vs. NaIO3.
Mentions: To explore the mechanism of mini-αA’s protective function against NaIO3-induced retinal degeneration, ARPE-19 cells were induced by NaIO3 at 2.5, 3 and 3.5 mM. Meanwhile, three subgroups treated with NaIO3 at 3.5 mM were set up, and were treated with mini-αA at concentrations of 10, 15 or 20 μM. Western blot analysis revealed that the expression of active-Caspase3 in cells treated with NaIO3 at 3.5 mM was much higher than that in normal cells, and the expression in cells treated with 3.5 mM NaIO3 and 20 μmM mini-αA decreased dramatically (Figure 4A).

Bottom Line: Further investigation into how mini-αA provided protection against NaIO3-induced RPE cell apoptosis showed that mini-αA reduced NaIO3-induced RPE cell apoptosis and autophagy.In addition, unfolded protein response was also involved in the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. mini-αA can antagonize RPE cell apoptosis induced by NaIO3.A possible mechanism is by inhibition of apoptosis by repressing autophagy and endoplasmic reticulum stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. zhjinglin@126.com.

ABSTRACT

Background: Studies have shown that mini-αA can protect retinal pigment epithelium (RPE) cells from apoptosis. However, no in vivo study concerning the anti-apoptotic function of mini-αA has been conducted yet.

Methods: MTT assay, HE staining and TUNEL assay were used to assess levels of cells, and an animal model was established to examine the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. Western blot analysis and RT-qPCR were performed to explore the possible mechanism of mini-αA's protective function against NaIO3-induced RPE cell apoptosis.

Results: RESULTS from in vivo and animal experiments showed that mini-αA antagonized NaIO3-induced RPE cell apoptosis. Further investigation into how mini-αA provided protection against NaIO3-induced RPE cell apoptosis showed that mini-αA reduced NaIO3-induced RPE cell apoptosis and autophagy. In addition, unfolded protein response was also involved in the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis.

Conclusions: mini-αA can antagonize RPE cell apoptosis induced by NaIO3. A possible mechanism is by inhibition of apoptosis by repressing autophagy and endoplasmic reticulum stress.

Show MeSH