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Rapid and sensitive identification of the herbal tea ingredient Taraxacum formosanum using loop-mediated isothermal amplification.

Lai GH, Chao J, Lin MK, Chang WT, Peng WH, Sun FC, Lee MS, Lee MS - Int J Mol Sci (2015)

Bottom Line: Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan.In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established.In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40402, Taiwan. just_dfm@hotmail.com.

ABSTRACT
Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDNA) of TF. LAMP amplicons were successfully amplified and detected when purified genomic DNA of TF was added in the LAMP reaction under isothermal condition (65 °C) within 45 min. These specific LAMP primers have high specificity and can accurately discriminate Taraxacum formosanum from other adulterant plants; 1 pg of genomic DNA was determined to be the detection limit of the LAMP assay. In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control.

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Related in: MedlinePlus

Diagnostic authentication of commercial plants of Taraxacum formosanum from various herbal markets using LAMP method (A) and PCR (B). Ten plants of Taraxacum formosanum obtained from various herbal markets were used for rapid authentication using the LAMP method after adding plant genomic DNA. Lane M, 1 kb of DNA ladder marker; lane N, genomic DNA of Ixeris chinensis; lane 1–10, ten commercial plant of Taraxacum formosanum from various herbal markets.
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ijms-16-01562-f006: Diagnostic authentication of commercial plants of Taraxacum formosanum from various herbal markets using LAMP method (A) and PCR (B). Ten plants of Taraxacum formosanum obtained from various herbal markets were used for rapid authentication using the LAMP method after adding plant genomic DNA. Lane M, 1 kb of DNA ladder marker; lane N, genomic DNA of Ixeris chinensis; lane 1–10, ten commercial plant of Taraxacum formosanum from various herbal markets.

Mentions: Based on the above results, this method was evaluated for use as a practical tool by authenticating ten commercially purchased TF plants from different herbal markets in Taiwan. Using genomic DNA extracted from these herbal samples as template, the LAMP product could be amplified from only two of the ten market samples of TF plants within 45 min under isothermal conditions (Figure 6A, lane 1 and lane 9). This result was also confirmed by PCR method (Figure 6B, lane 1 and lane 9). This result indicates that 80% of commercial TF samples are obviously misrepresented.


Rapid and sensitive identification of the herbal tea ingredient Taraxacum formosanum using loop-mediated isothermal amplification.

Lai GH, Chao J, Lin MK, Chang WT, Peng WH, Sun FC, Lee MS, Lee MS - Int J Mol Sci (2015)

Diagnostic authentication of commercial plants of Taraxacum formosanum from various herbal markets using LAMP method (A) and PCR (B). Ten plants of Taraxacum formosanum obtained from various herbal markets were used for rapid authentication using the LAMP method after adding plant genomic DNA. Lane M, 1 kb of DNA ladder marker; lane N, genomic DNA of Ixeris chinensis; lane 1–10, ten commercial plant of Taraxacum formosanum from various herbal markets.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307320&req=5

ijms-16-01562-f006: Diagnostic authentication of commercial plants of Taraxacum formosanum from various herbal markets using LAMP method (A) and PCR (B). Ten plants of Taraxacum formosanum obtained from various herbal markets were used for rapid authentication using the LAMP method after adding plant genomic DNA. Lane M, 1 kb of DNA ladder marker; lane N, genomic DNA of Ixeris chinensis; lane 1–10, ten commercial plant of Taraxacum formosanum from various herbal markets.
Mentions: Based on the above results, this method was evaluated for use as a practical tool by authenticating ten commercially purchased TF plants from different herbal markets in Taiwan. Using genomic DNA extracted from these herbal samples as template, the LAMP product could be amplified from only two of the ten market samples of TF plants within 45 min under isothermal conditions (Figure 6A, lane 1 and lane 9). This result was also confirmed by PCR method (Figure 6B, lane 1 and lane 9). This result indicates that 80% of commercial TF samples are obviously misrepresented.

Bottom Line: Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan.In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established.In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40402, Taiwan. just_dfm@hotmail.com.

ABSTRACT
Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDNA) of TF. LAMP amplicons were successfully amplified and detected when purified genomic DNA of TF was added in the LAMP reaction under isothermal condition (65 °C) within 45 min. These specific LAMP primers have high specificity and can accurately discriminate Taraxacum formosanum from other adulterant plants; 1 pg of genomic DNA was determined to be the detection limit of the LAMP assay. In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control.

Show MeSH
Related in: MedlinePlus