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Membrane trafficking in the yeast Saccharomyces cerevisiae model.

Feyder S, De Craene JO, Bär S, Bertazzi DL, Friant S - Int J Mol Sci (2015)

Bottom Line: They have isolated yeast sec mutants unable to secrete an extracellular enzyme and these SEC genes were identified as encoding key effectors of the secretory machinery.Plasma membrane proteins can be internalized by endocytosis (END) and transported to endosomes where they are sorted between those targeted for vacuolar degradation and those redirected to the Golgi (recycling or RCY pathway).Studies in yeast S. cerevisiae allowed the identification of most of the known effectors, protein complexes, and trafficking pathways in eukaryotic cells, and most of them are conserved among eukaryotes.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Genetics, UMR7156, Université de Strasbourg and CNRS, 21 rue Descartes, Strasbourg 67084, France. sergefeyder@hotmail.com.

ABSTRACT
The yeast Saccharomyces cerevisiae is one of the best characterized eukaryotic models. The secretory pathway was the first trafficking pathway clearly understood mainly thanks to the work done in the laboratory of Randy Schekman in the 1980s. They have isolated yeast sec mutants unable to secrete an extracellular enzyme and these SEC genes were identified as encoding key effectors of the secretory machinery. For this work, the 2013 Nobel Prize in Physiology and Medicine has been awarded to Randy Schekman; the prize is shared with James Rothman and Thomas Südhof. Here, we present the different trafficking pathways of yeast S. cerevisiae. At the Golgi apparatus newly synthesized proteins are sorted between those transported to the plasma membrane (PM), or the external medium, via the exocytosis or secretory pathway (SEC), and those targeted to the vacuole either through endosomes (vacuolar protein sorting or VPS pathway) or directly (alkaline phosphatase or ALP pathway). Plasma membrane proteins can be internalized by endocytosis (END) and transported to endosomes where they are sorted between those targeted for vacuolar degradation and those redirected to the Golgi (recycling or RCY pathway). Studies in yeast S. cerevisiae allowed the identification of most of the known effectors, protein complexes, and trafficking pathways in eukaryotic cells, and most of them are conserved among eukaryotes.

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Representation of the different yeast intracellular trafficking pathways. Organellar soluble and membrane proteins are synthesized at the endoplasmic reticulum (ER) and transported to the unstacked Golgi (grey arrow). At the Golgi, these proteins are sorted into anterograde transport vesicles for ER resident proteins (grey arrow), into secretory (SEC) vesicles for plasma membrane (PM) and extracellular proteins (blue arrow), and into vacuolar protein sorting (VPS) vesicles for vacuolar proteins passing through endosomes (red arrow). The endocytic pathway (END) is used for internalization of PM proteins and extracellular medium components (green arrow). At the early endosomes (EE), proteins are sorted between those targeted for degradation into the vacuole, after maturation of the EE into the late endosome or multivesicular body (MVB), and those that are following the recycling pathway (RCY) to avoid degradation by being targeted to the Golgi (orange arrow).
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ijms-16-01509-f002: Representation of the different yeast intracellular trafficking pathways. Organellar soluble and membrane proteins are synthesized at the endoplasmic reticulum (ER) and transported to the unstacked Golgi (grey arrow). At the Golgi, these proteins are sorted into anterograde transport vesicles for ER resident proteins (grey arrow), into secretory (SEC) vesicles for plasma membrane (PM) and extracellular proteins (blue arrow), and into vacuolar protein sorting (VPS) vesicles for vacuolar proteins passing through endosomes (red arrow). The endocytic pathway (END) is used for internalization of PM proteins and extracellular medium components (green arrow). At the early endosomes (EE), proteins are sorted between those targeted for degradation into the vacuole, after maturation of the EE into the late endosome or multivesicular body (MVB), and those that are following the recycling pathway (RCY) to avoid degradation by being targeted to the Golgi (orange arrow).

Mentions: Its first advantage is the shared complex intracellular organization with higher eukaryotes (Figure 2), as illustrated by its many fundamental contributions to the study of cellular processes such as cell signaling, membrane trafficking, lipid metabolism, and mitochondria import amongst others.


Membrane trafficking in the yeast Saccharomyces cerevisiae model.

Feyder S, De Craene JO, Bär S, Bertazzi DL, Friant S - Int J Mol Sci (2015)

Representation of the different yeast intracellular trafficking pathways. Organellar soluble and membrane proteins are synthesized at the endoplasmic reticulum (ER) and transported to the unstacked Golgi (grey arrow). At the Golgi, these proteins are sorted into anterograde transport vesicles for ER resident proteins (grey arrow), into secretory (SEC) vesicles for plasma membrane (PM) and extracellular proteins (blue arrow), and into vacuolar protein sorting (VPS) vesicles for vacuolar proteins passing through endosomes (red arrow). The endocytic pathway (END) is used for internalization of PM proteins and extracellular medium components (green arrow). At the early endosomes (EE), proteins are sorted between those targeted for degradation into the vacuole, after maturation of the EE into the late endosome or multivesicular body (MVB), and those that are following the recycling pathway (RCY) to avoid degradation by being targeted to the Golgi (orange arrow).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307317&req=5

ijms-16-01509-f002: Representation of the different yeast intracellular trafficking pathways. Organellar soluble and membrane proteins are synthesized at the endoplasmic reticulum (ER) and transported to the unstacked Golgi (grey arrow). At the Golgi, these proteins are sorted into anterograde transport vesicles for ER resident proteins (grey arrow), into secretory (SEC) vesicles for plasma membrane (PM) and extracellular proteins (blue arrow), and into vacuolar protein sorting (VPS) vesicles for vacuolar proteins passing through endosomes (red arrow). The endocytic pathway (END) is used for internalization of PM proteins and extracellular medium components (green arrow). At the early endosomes (EE), proteins are sorted between those targeted for degradation into the vacuole, after maturation of the EE into the late endosome or multivesicular body (MVB), and those that are following the recycling pathway (RCY) to avoid degradation by being targeted to the Golgi (orange arrow).
Mentions: Its first advantage is the shared complex intracellular organization with higher eukaryotes (Figure 2), as illustrated by its many fundamental contributions to the study of cellular processes such as cell signaling, membrane trafficking, lipid metabolism, and mitochondria import amongst others.

Bottom Line: They have isolated yeast sec mutants unable to secrete an extracellular enzyme and these SEC genes were identified as encoding key effectors of the secretory machinery.Plasma membrane proteins can be internalized by endocytosis (END) and transported to endosomes where they are sorted between those targeted for vacuolar degradation and those redirected to the Golgi (recycling or RCY pathway).Studies in yeast S. cerevisiae allowed the identification of most of the known effectors, protein complexes, and trafficking pathways in eukaryotic cells, and most of them are conserved among eukaryotes.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Genetics, UMR7156, Université de Strasbourg and CNRS, 21 rue Descartes, Strasbourg 67084, France. sergefeyder@hotmail.com.

ABSTRACT
The yeast Saccharomyces cerevisiae is one of the best characterized eukaryotic models. The secretory pathway was the first trafficking pathway clearly understood mainly thanks to the work done in the laboratory of Randy Schekman in the 1980s. They have isolated yeast sec mutants unable to secrete an extracellular enzyme and these SEC genes were identified as encoding key effectors of the secretory machinery. For this work, the 2013 Nobel Prize in Physiology and Medicine has been awarded to Randy Schekman; the prize is shared with James Rothman and Thomas Südhof. Here, we present the different trafficking pathways of yeast S. cerevisiae. At the Golgi apparatus newly synthesized proteins are sorted between those transported to the plasma membrane (PM), or the external medium, via the exocytosis or secretory pathway (SEC), and those targeted to the vacuole either through endosomes (vacuolar protein sorting or VPS pathway) or directly (alkaline phosphatase or ALP pathway). Plasma membrane proteins can be internalized by endocytosis (END) and transported to endosomes where they are sorted between those targeted for vacuolar degradation and those redirected to the Golgi (recycling or RCY pathway). Studies in yeast S. cerevisiae allowed the identification of most of the known effectors, protein complexes, and trafficking pathways in eukaryotic cells, and most of them are conserved among eukaryotes.

Show MeSH
Related in: MedlinePlus