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Hair dyes resorcinol and lawsone reduce production of melanin in melanoma cells by tyrosinase activity inhibition and decreasing tyrosinase and microphthalmia-associated transcription factor (MITF) expression.

Lee SM, Chen YS, Lin CC, Chen KH - Int J Mol Sci (2015)

Bottom Line: The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin.The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro.Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan. s107@eip.mkc.edu.tw.

ABSTRACT
Hair coloring products are one of the most important cosmetics for modern people; there are three major types of hair dyes, including the temporary, semi-permanent and permanent hair dyes. The selected hair dyes (such as ammonium persulfate, sodium persulfate, resorcinol and lawsone) are the important components for hair coloring products. Therefore, we analyzed the effects of these compounds on melanogenesis in B16-F10 melanoma cells. The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin. The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro. Resorcinol and lawsone can also downregulate the protein levels of tyrosinase and microphthalmia-associated transcription factor (MITF) in B16-F10 cells. Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles. Moreover, resorcinol and lawsone may also be used as hypopigmenting agents to food, agricultural and cosmetic industry in the future.

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Related in: MedlinePlus

Effects of hair dyes (A) resorcinol and (B) lawsone on the protein levels of MITF and tyrosinase in B16-F10 cells. The protein level of GAPDH is used as an internal control. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.
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ijms-16-01495-f007: Effects of hair dyes (A) resorcinol and (B) lawsone on the protein levels of MITF and tyrosinase in B16-F10 cells. The protein level of GAPDH is used as an internal control. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.

Mentions: To further check the mechanism of resorcinol and lawsone on the inhibition of melanin production, we tested the levels of the most important melanogenesis regulation proteins, MITF and tyrosinase, in resorcinol and lawsone treated B16-F10 cells. Results are shown in Figure 7; the quantitative results are shown in lower figures. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein was used as an internal control for normalization. For resorcinol, GAPDH protein levels are equal in all samples, but MITF are slightly decreased with the increasing concentrations of resorcinol. At 2 mM resorcinol treatment condition, the level of MITF diminished to the lowest amount (Figure 7A). Tyrosinase protein levels are significantly reduced with the treatments of resorcinol. Tyrosinase levels are clearly downregulated by resorcinol if the concentrations are higher than 1.5 mM (Figure 7A). For lawsone, the result is shown in Figure 7B; GAPDH proteins are also equivalent in all treatments. However, both MITF and tyrosinase proteins in B16-F10 cells are evidently repressed by the treatments of lawsone. The reducing effects of lawsone are noticeable when its concentrations are higher than 50 μM (Figure 7B). Therefore, we suggest that resorcinol and lawsone have functions to reduce the protein levels of MITF and tyrosinase in B16-F10 cells.


Hair dyes resorcinol and lawsone reduce production of melanin in melanoma cells by tyrosinase activity inhibition and decreasing tyrosinase and microphthalmia-associated transcription factor (MITF) expression.

Lee SM, Chen YS, Lin CC, Chen KH - Int J Mol Sci (2015)

Effects of hair dyes (A) resorcinol and (B) lawsone on the protein levels of MITF and tyrosinase in B16-F10 cells. The protein level of GAPDH is used as an internal control. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307316&req=5

ijms-16-01495-f007: Effects of hair dyes (A) resorcinol and (B) lawsone on the protein levels of MITF and tyrosinase in B16-F10 cells. The protein level of GAPDH is used as an internal control. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.
Mentions: To further check the mechanism of resorcinol and lawsone on the inhibition of melanin production, we tested the levels of the most important melanogenesis regulation proteins, MITF and tyrosinase, in resorcinol and lawsone treated B16-F10 cells. Results are shown in Figure 7; the quantitative results are shown in lower figures. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein was used as an internal control for normalization. For resorcinol, GAPDH protein levels are equal in all samples, but MITF are slightly decreased with the increasing concentrations of resorcinol. At 2 mM resorcinol treatment condition, the level of MITF diminished to the lowest amount (Figure 7A). Tyrosinase protein levels are significantly reduced with the treatments of resorcinol. Tyrosinase levels are clearly downregulated by resorcinol if the concentrations are higher than 1.5 mM (Figure 7A). For lawsone, the result is shown in Figure 7B; GAPDH proteins are also equivalent in all treatments. However, both MITF and tyrosinase proteins in B16-F10 cells are evidently repressed by the treatments of lawsone. The reducing effects of lawsone are noticeable when its concentrations are higher than 50 μM (Figure 7B). Therefore, we suggest that resorcinol and lawsone have functions to reduce the protein levels of MITF and tyrosinase in B16-F10 cells.

Bottom Line: The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin.The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro.Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan. s107@eip.mkc.edu.tw.

ABSTRACT
Hair coloring products are one of the most important cosmetics for modern people; there are three major types of hair dyes, including the temporary, semi-permanent and permanent hair dyes. The selected hair dyes (such as ammonium persulfate, sodium persulfate, resorcinol and lawsone) are the important components for hair coloring products. Therefore, we analyzed the effects of these compounds on melanogenesis in B16-F10 melanoma cells. The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin. The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro. Resorcinol and lawsone can also downregulate the protein levels of tyrosinase and microphthalmia-associated transcription factor (MITF) in B16-F10 cells. Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles. Moreover, resorcinol and lawsone may also be used as hypopigmenting agents to food, agricultural and cosmetic industry in the future.

Show MeSH
Related in: MedlinePlus