Limits...
Hair dyes resorcinol and lawsone reduce production of melanin in melanoma cells by tyrosinase activity inhibition and decreasing tyrosinase and microphthalmia-associated transcription factor (MITF) expression.

Lee SM, Chen YS, Lin CC, Chen KH - Int J Mol Sci (2015)

Bottom Line: The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin.The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro.Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan. s107@eip.mkc.edu.tw.

ABSTRACT
Hair coloring products are one of the most important cosmetics for modern people; there are three major types of hair dyes, including the temporary, semi-permanent and permanent hair dyes. The selected hair dyes (such as ammonium persulfate, sodium persulfate, resorcinol and lawsone) are the important components for hair coloring products. Therefore, we analyzed the effects of these compounds on melanogenesis in B16-F10 melanoma cells. The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin. The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro. Resorcinol and lawsone can also downregulate the protein levels of tyrosinase and microphthalmia-associated transcription factor (MITF) in B16-F10 cells. Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles. Moreover, resorcinol and lawsone may also be used as hypopigmenting agents to food, agricultural and cosmetic industry in the future.

Show MeSH

Related in: MedlinePlus

Effects of hair dyes on the cellular tyrosinase activity in B16-F10 cells. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4307316&req=5

ijms-16-01495-f006: Effects of hair dyes on the cellular tyrosinase activity in B16-F10 cells. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.

Mentions: To further examine the anti-melanogenesis functions of the selected hair dyes, we analyzed the effects of the hair dyes on tyrosinase in B16-F10 Cells using a zymographic assay. The B16-F10 cells were firstly treated with various concentrations of the samples for 24 h, and then the cellular proteins were extracted to analyze the tyrosinase activity of protein samples. This analysis explains the effect of tested compound on the cellular tyrosinase within cells. The results are shown in Figure 6. For ammonium persulfate and sodium persulfate, the compounds did not significantly affect the formation of melanin-containing dark bands in all tested samples from B16-F10 cells (Figure 6A,B). The results revealed that ammonium persulfate and sodium persulfate do not reduce the activity of cellular tyrosinase. In addition, resorcinol was found to decrease the dark bands in a dose-dependent manner; the inhibition ratio is approximately 0.6-fold of control at the condition of 2 mM resorcinol treatment (Figure 6C). Similar to the results of resorcinol, lawsone was found to attenuate the melanin-containing dark bands in all treatments. At the condition of 70 μM lawsone treatment, the band intensity decreased to approximately 0.45-fold of control (Figure 6D). Therefore, lawsone has the ability to influence tyrosinase activity in B16-F10 cells.


Hair dyes resorcinol and lawsone reduce production of melanin in melanoma cells by tyrosinase activity inhibition and decreasing tyrosinase and microphthalmia-associated transcription factor (MITF) expression.

Lee SM, Chen YS, Lin CC, Chen KH - Int J Mol Sci (2015)

Effects of hair dyes on the cellular tyrosinase activity in B16-F10 cells. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307316&req=5

ijms-16-01495-f006: Effects of hair dyes on the cellular tyrosinase activity in B16-F10 cells. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 3). *p < 0.05 compared with the control.
Mentions: To further examine the anti-melanogenesis functions of the selected hair dyes, we analyzed the effects of the hair dyes on tyrosinase in B16-F10 Cells using a zymographic assay. The B16-F10 cells were firstly treated with various concentrations of the samples for 24 h, and then the cellular proteins were extracted to analyze the tyrosinase activity of protein samples. This analysis explains the effect of tested compound on the cellular tyrosinase within cells. The results are shown in Figure 6. For ammonium persulfate and sodium persulfate, the compounds did not significantly affect the formation of melanin-containing dark bands in all tested samples from B16-F10 cells (Figure 6A,B). The results revealed that ammonium persulfate and sodium persulfate do not reduce the activity of cellular tyrosinase. In addition, resorcinol was found to decrease the dark bands in a dose-dependent manner; the inhibition ratio is approximately 0.6-fold of control at the condition of 2 mM resorcinol treatment (Figure 6C). Similar to the results of resorcinol, lawsone was found to attenuate the melanin-containing dark bands in all treatments. At the condition of 70 μM lawsone treatment, the band intensity decreased to approximately 0.45-fold of control (Figure 6D). Therefore, lawsone has the ability to influence tyrosinase activity in B16-F10 cells.

Bottom Line: The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin.The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro.Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan. s107@eip.mkc.edu.tw.

ABSTRACT
Hair coloring products are one of the most important cosmetics for modern people; there are three major types of hair dyes, including the temporary, semi-permanent and permanent hair dyes. The selected hair dyes (such as ammonium persulfate, sodium persulfate, resorcinol and lawsone) are the important components for hair coloring products. Therefore, we analyzed the effects of these compounds on melanogenesis in B16-F10 melanoma cells. The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin. The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro. Resorcinol and lawsone can also downregulate the protein levels of tyrosinase and microphthalmia-associated transcription factor (MITF) in B16-F10 cells. Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles. Moreover, resorcinol and lawsone may also be used as hypopigmenting agents to food, agricultural and cosmetic industry in the future.

Show MeSH
Related in: MedlinePlus