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Hair dyes resorcinol and lawsone reduce production of melanin in melanoma cells by tyrosinase activity inhibition and decreasing tyrosinase and microphthalmia-associated transcription factor (MITF) expression.

Lee SM, Chen YS, Lin CC, Chen KH - Int J Mol Sci (2015)

Bottom Line: The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin.The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro.Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan. s107@eip.mkc.edu.tw.

ABSTRACT
Hair coloring products are one of the most important cosmetics for modern people; there are three major types of hair dyes, including the temporary, semi-permanent and permanent hair dyes. The selected hair dyes (such as ammonium persulfate, sodium persulfate, resorcinol and lawsone) are the important components for hair coloring products. Therefore, we analyzed the effects of these compounds on melanogenesis in B16-F10 melanoma cells. The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin. The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro. Resorcinol and lawsone can also downregulate the protein levels of tyrosinase and microphthalmia-associated transcription factor (MITF) in B16-F10 cells. Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles. Moreover, resorcinol and lawsone may also be used as hypopigmenting agents to food, agricultural and cosmetic industry in the future.

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Related in: MedlinePlus

In vitro effects of hair dyes on the activity of B16-F10 cellular tyrosinase. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 6). *p < 0.05 compared with the control.
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ijms-16-01495-f005: In vitro effects of hair dyes on the activity of B16-F10 cellular tyrosinase. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 6). *p < 0.05 compared with the control.

Mentions: To test whether the reducing effect of selected hair dyes on melanogenesis is mediated by direct inhibition of tyrosinase activity, the hair dyes were used to check the in vitro anti-tyrosinase activity on both purified mushroom tyrosinase and extracted B16-F10 cellular tyrosinase; the results are shown in Figure 4 and Figure 5, respectively.


Hair dyes resorcinol and lawsone reduce production of melanin in melanoma cells by tyrosinase activity inhibition and decreasing tyrosinase and microphthalmia-associated transcription factor (MITF) expression.

Lee SM, Chen YS, Lin CC, Chen KH - Int J Mol Sci (2015)

In vitro effects of hair dyes on the activity of B16-F10 cellular tyrosinase. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 6). *p < 0.05 compared with the control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307316&req=5

ijms-16-01495-f005: In vitro effects of hair dyes on the activity of B16-F10 cellular tyrosinase. (A) ammonium persulfate; (B) sodium persulfate; (C) resorcinol and (D) lawsone. Each value is expressed as the mean ± S.E. (n = 6). *p < 0.05 compared with the control.
Mentions: To test whether the reducing effect of selected hair dyes on melanogenesis is mediated by direct inhibition of tyrosinase activity, the hair dyes were used to check the in vitro anti-tyrosinase activity on both purified mushroom tyrosinase and extracted B16-F10 cellular tyrosinase; the results are shown in Figure 4 and Figure 5, respectively.

Bottom Line: The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin.The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro.Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles.

View Article: PubMed Central - PubMed

Affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan. s107@eip.mkc.edu.tw.

ABSTRACT
Hair coloring products are one of the most important cosmetics for modern people; there are three major types of hair dyes, including the temporary, semi-permanent and permanent hair dyes. The selected hair dyes (such as ammonium persulfate, sodium persulfate, resorcinol and lawsone) are the important components for hair coloring products. Therefore, we analyzed the effects of these compounds on melanogenesis in B16-F10 melanoma cells. The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin. The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro. Resorcinol and lawsone can also downregulate the protein levels of tyrosinase and microphthalmia-associated transcription factor (MITF) in B16-F10 cells. Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles. Moreover, resorcinol and lawsone may also be used as hypopigmenting agents to food, agricultural and cosmetic industry in the future.

Show MeSH
Related in: MedlinePlus