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MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

Bottom Line: Many miRNAs were detected as significantly differentially expressed between the two libraries.These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance.The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

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Hierarchical cluster analysis for lactation-related miRNAs (A) and target genes (B) (The color represent the expression level, green means low level red means high level; the number of the phylogenetic tree represents the evolutionary relationship of the genes, the smaller, the closer.)
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ijms-16-01448-f008: Hierarchical cluster analysis for lactation-related miRNAs (A) and target genes (B) (The color represent the expression level, green means low level red means high level; the number of the phylogenetic tree represents the evolutionary relationship of the genes, the smaller, the closer.)

Mentions: Hierarchical cluster analysis for 17 lactation-related miRNAs expressed in mammary gland from Jinhua and Yorkshire lactation pigs was performed, and these miRNAs were found to be differentially expressed in the two libraries (Figure 8A). Some of these miRNAs were reported to be immune-related, like the miR-30 family, which can play a critical role in the development of the infant immune system [14]. Some miRNAs were shown to be related to mammary gland development and breast cancer. Therefore, we use Targetscan and Miranda to identify putative targets of these selected miRNAs. For example, DNMT1, PTEN and WNT1 were the putative target genes of miR-148/152 family. STAT3 is a target gene of miR-21 and PCNA is the target of miR-24-3p. Interestingly, many of these target genes are related to the proliferation and viability of mammary epithelial cells. GHR was demonstrated to play a key role in mammary gland development in many studies [15]. Thus, these related genes were then selected to perform hierarchical cluster analysis based on our previous mRNA sequencing data to analyze the expression pattern between the two breeds (Figure 8B). The significant differences in the expression of miRNAs and their targets between the two periods suggested the possible biological role miRNAs might play in mammary gland development and lactation.


MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

Hierarchical cluster analysis for lactation-related miRNAs (A) and target genes (B) (The color represent the expression level, green means low level red means high level; the number of the phylogenetic tree represents the evolutionary relationship of the genes, the smaller, the closer.)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307312&req=5

ijms-16-01448-f008: Hierarchical cluster analysis for lactation-related miRNAs (A) and target genes (B) (The color represent the expression level, green means low level red means high level; the number of the phylogenetic tree represents the evolutionary relationship of the genes, the smaller, the closer.)
Mentions: Hierarchical cluster analysis for 17 lactation-related miRNAs expressed in mammary gland from Jinhua and Yorkshire lactation pigs was performed, and these miRNAs were found to be differentially expressed in the two libraries (Figure 8A). Some of these miRNAs were reported to be immune-related, like the miR-30 family, which can play a critical role in the development of the infant immune system [14]. Some miRNAs were shown to be related to mammary gland development and breast cancer. Therefore, we use Targetscan and Miranda to identify putative targets of these selected miRNAs. For example, DNMT1, PTEN and WNT1 were the putative target genes of miR-148/152 family. STAT3 is a target gene of miR-21 and PCNA is the target of miR-24-3p. Interestingly, many of these target genes are related to the proliferation and viability of mammary epithelial cells. GHR was demonstrated to play a key role in mammary gland development in many studies [15]. Thus, these related genes were then selected to perform hierarchical cluster analysis based on our previous mRNA sequencing data to analyze the expression pattern between the two breeds (Figure 8B). The significant differences in the expression of miRNAs and their targets between the two periods suggested the possible biological role miRNAs might play in mammary gland development and lactation.

Bottom Line: Many miRNAs were detected as significantly differentially expressed between the two libraries.These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance.The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

Show MeSH