Limits...
MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

Bottom Line: Many miRNAs were detected as significantly differentially expressed between the two libraries.These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance.The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

Show MeSH
Partial GO enrichment for the predicted target genes in cellular component (A); molecular function (B) and biological processes (C).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4307312&req=5

ijms-16-01448-f007: Partial GO enrichment for the predicted target genes in cellular component (A); molecular function (B) and biological processes (C).

Mentions: Target gene prediction was performed to further understand the physiological functions and biological processes involving these miRNAs during mammary gland development and lactation, (Figure 7) based on miRNA/mRNA interactions to provide some molecular insight into the processes. The figure shows partial GO enrichment for the predicted target genes in cellular component, molecular function and biological processes. The enriched GO targets of the miRNAs were mainly associated with the nucleus, protein binding and transport items. The predicted target genes were classified according to KEGG function annotations to identify the pathways that were actively regulated by miRNAs in mammary gland (Tables S2 and S3). The involved pathways including the calcium signaling pathway, endocytosis, axon guidance, antigen processing and presentation, toll-like receptor signaling pathway, and the mammalian target of rapamycin (mTOR) signaling pathway may play important roles in the regulation of mammary gland differentiation and lactation.


MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

Partial GO enrichment for the predicted target genes in cellular component (A); molecular function (B) and biological processes (C).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307312&req=5

ijms-16-01448-f007: Partial GO enrichment for the predicted target genes in cellular component (A); molecular function (B) and biological processes (C).
Mentions: Target gene prediction was performed to further understand the physiological functions and biological processes involving these miRNAs during mammary gland development and lactation, (Figure 7) based on miRNA/mRNA interactions to provide some molecular insight into the processes. The figure shows partial GO enrichment for the predicted target genes in cellular component, molecular function and biological processes. The enriched GO targets of the miRNAs were mainly associated with the nucleus, protein binding and transport items. The predicted target genes were classified according to KEGG function annotations to identify the pathways that were actively regulated by miRNAs in mammary gland (Tables S2 and S3). The involved pathways including the calcium signaling pathway, endocytosis, axon guidance, antigen processing and presentation, toll-like receptor signaling pathway, and the mammalian target of rapamycin (mTOR) signaling pathway may play important roles in the regulation of mammary gland differentiation and lactation.

Bottom Line: Many miRNAs were detected as significantly differentially expressed between the two libraries.These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance.The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

Show MeSH