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MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

Bottom Line: However, little is known about porcine mammary gland miRNAs.In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire.Many miRNAs were detected as significantly differentially expressed between the two libraries.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

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(A) Pie chart for Rfam RX_J and RX_Y and (B) Length distribution analysis.
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ijms-16-01448-f003: (A) Pie chart for Rfam RX_J and RX_Y and (B) Length distribution analysis.

Mentions: We separated out and discarded rRNA, tRNA, snRNA, scRNA, srpRNA and snoRNA sequences, which were identified using a BLAST against the known noncoding RNAs deposited in the Rfam and NCBI GenBank databases (Figure 3A).


MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

(A) Pie chart for Rfam RX_J and RX_Y and (B) Length distribution analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307312&req=5

ijms-16-01448-f003: (A) Pie chart for Rfam RX_J and RX_Y and (B) Length distribution analysis.
Mentions: We separated out and discarded rRNA, tRNA, snRNA, scRNA, srpRNA and snoRNA sequences, which were identified using a BLAST against the known noncoding RNAs deposited in the Rfam and NCBI GenBank databases (Figure 3A).

Bottom Line: However, little is known about porcine mammary gland miRNAs.In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire.Many miRNAs were detected as significantly differentially expressed between the two libraries.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

Show MeSH
Related in: MedlinePlus