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MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

Bottom Line: Many miRNAs were detected as significantly differentially expressed between the two libraries.These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance.The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

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The microstructure and gene expression of lactating mammary gland tissue in Jinhua and Yorkshire pig breeds. (A) Paraffin section of Jinhua mammary gland in the 21-day lactation period; (B) Paraffin section of Yorkshire mammary gland in the 21-day lactation period; (C) Expression of casein in Jinhua lactating mammary gland; (D) Expression of casein in Yorkshire lactating mammary glands; (E) Expression of cytokeratin in lactating Jinhua mammary glands and (F) Expression of cytokeratin in lactating Yorkshire mammary glands.
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ijms-16-01448-f001: The microstructure and gene expression of lactating mammary gland tissue in Jinhua and Yorkshire pig breeds. (A) Paraffin section of Jinhua mammary gland in the 21-day lactation period; (B) Paraffin section of Yorkshire mammary gland in the 21-day lactation period; (C) Expression of casein in Jinhua lactating mammary gland; (D) Expression of casein in Yorkshire lactating mammary glands; (E) Expression of cytokeratin in lactating Jinhua mammary glands and (F) Expression of cytokeratin in lactating Yorkshire mammary glands.

Mentions: Hematoxylin and eosin (HE) staining, immunohistochemistry and immunofluorescence were all employed to verify the microstructure of the lactating mammary gland tissues used in constructing miRNA libraries. In the lactation mammary glands, many mature alveolar structures were packed with mammary lobules of a variety of shapes. Mature alveolar lumens seemed larger in Yorkshire than Jinhua in appearance and many of the lumens in both breeds were filled with secretions, with little connective tissue between alveoli (Figure 1A,B), and certain amounts of α-casein were found surrounding the nuclei and in the large alveolus. Additionally, α-casein expression level in Jinhua was comparatively lower than Yorkshire (Figure 1C,D). Furthermore, expression of cytokeratin 18 (CK-18), a marker of epithelial cells, was also detected in both mammary gland tissues (Figure 1E,F).


MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.

Peng J, Zhao JS, Shen YF, Mao HG, Xu NY - Int J Mol Sci (2015)

The microstructure and gene expression of lactating mammary gland tissue in Jinhua and Yorkshire pig breeds. (A) Paraffin section of Jinhua mammary gland in the 21-day lactation period; (B) Paraffin section of Yorkshire mammary gland in the 21-day lactation period; (C) Expression of casein in Jinhua lactating mammary gland; (D) Expression of casein in Yorkshire lactating mammary glands; (E) Expression of cytokeratin in lactating Jinhua mammary glands and (F) Expression of cytokeratin in lactating Yorkshire mammary glands.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307312&req=5

ijms-16-01448-f001: The microstructure and gene expression of lactating mammary gland tissue in Jinhua and Yorkshire pig breeds. (A) Paraffin section of Jinhua mammary gland in the 21-day lactation period; (B) Paraffin section of Yorkshire mammary gland in the 21-day lactation period; (C) Expression of casein in Jinhua lactating mammary gland; (D) Expression of casein in Yorkshire lactating mammary glands; (E) Expression of cytokeratin in lactating Jinhua mammary glands and (F) Expression of cytokeratin in lactating Yorkshire mammary glands.
Mentions: Hematoxylin and eosin (HE) staining, immunohistochemistry and immunofluorescence were all employed to verify the microstructure of the lactating mammary gland tissues used in constructing miRNA libraries. In the lactation mammary glands, many mature alveolar structures were packed with mammary lobules of a variety of shapes. Mature alveolar lumens seemed larger in Yorkshire than Jinhua in appearance and many of the lumens in both breeds were filled with secretions, with little connective tissue between alveoli (Figure 1A,B), and certain amounts of α-casein were found surrounding the nuclei and in the large alveolus. Additionally, α-casein expression level in Jinhua was comparatively lower than Yorkshire (Figure 1C,D). Furthermore, expression of cytokeratin 18 (CK-18), a marker of epithelial cells, was also detected in both mammary gland tissues (Figure 1E,F).

Bottom Line: Many miRNAs were detected as significantly differentially expressed between the two libraries.These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance.The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.

ABSTRACT
MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.

Show MeSH