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Anti-inflammatory and analgesic effects of pyeongwisan on LPS-stimulated murine macrophages and mouse models of acetic acid-induced writhing response and xylene-induced ear edema.

Oh YC, Jeong YH, Cho WK, Ha JH, Gu MJ, Ma JY - Int J Mol Sci (2015)

Bottom Line: However, its effects on inflammation-related diseases are unknown.We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time.Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine (KM)-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong, Daejeon 305-811, Korea. ulivuli@kiom.re.kr.

ABSTRACT
Pyeongwisan (PW) is an herbal medication used in traditional East Asian medicine to treat anorexia, abdominal distension, borborygmus and diarrhea caused by gastric catarrh, atony and dilatation. However, its effects on inflammation-related diseases are unknown. In this study, we investigated the biological effects of PW on lipopolysaccharide (LPS)-mediated inflammation in macrophages and on local inflammation in vivo. We investigated the biological effects of PW on the production of inflammatory mediators, pro-inflammatory cytokines and related products as well as the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated macrophages. Additionally, we evaluated the analgesic effect on the acetic acid-induced writhing response and the inhibitory activity on xylene-induced ear edema in mice. PW showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and interleukin-1β (IL-1β). In addition, PW strongly suppressed inducible nitric oxide synthase (iNOS), a NO synthesis enzyme, induced heme oxygenase-1 (HO-1) expression and inhibited NF-κB activation and MAPK phosphorylation. Also, PW suppressed TNF-α, IL-6 and IL-1β cytokine production in LPS-stimulated peritoneal macrophage cells. Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema. We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time. Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

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HPLC-DAD spectra and HPLC chromatograms of the main constituent compounds of PW. (A) Standard mixture and (B) PW at 230 nm. (1) hesperidin, 16.64 min; (2) glycyrrhizin, 29.74 min; (3) atractylenolide III, 37.46 min.
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ijms-16-01232-f007: HPLC-DAD spectra and HPLC chromatograms of the main constituent compounds of PW. (A) Standard mixture and (B) PW at 230 nm. (1) hesperidin, 16.64 min; (2) glycyrrhizin, 29.74 min; (3) atractylenolide III, 37.46 min.

Mentions: The results of HPLC-DAD analysis, using the mobile phases consisting of 0.1% aqueous trifluoroacetic acid (v/v) and pure acetonitrile with a gradient flow, showed satisfactory separation (Table 1). Optimal absorbance for all analytes (hesperidin, 201 nm; glycyrrhizin, 250 nm; atractylenolide III, 222 nm) was detected at 230 nm (Figure 7). Three of the retention time peaks (hesperidin, 16.64 min; glycyrrhizin, 29.74 min; atractylenolide III, 37.46 min) showed chromatograms of the reference components and a 60% methanol extract of PW (Figure 7). These compounds were identified qualitatively by comparing the retention times and UV wavelengths with those of standard compounds.


Anti-inflammatory and analgesic effects of pyeongwisan on LPS-stimulated murine macrophages and mouse models of acetic acid-induced writhing response and xylene-induced ear edema.

Oh YC, Jeong YH, Cho WK, Ha JH, Gu MJ, Ma JY - Int J Mol Sci (2015)

HPLC-DAD spectra and HPLC chromatograms of the main constituent compounds of PW. (A) Standard mixture and (B) PW at 230 nm. (1) hesperidin, 16.64 min; (2) glycyrrhizin, 29.74 min; (3) atractylenolide III, 37.46 min.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307301&req=5

ijms-16-01232-f007: HPLC-DAD spectra and HPLC chromatograms of the main constituent compounds of PW. (A) Standard mixture and (B) PW at 230 nm. (1) hesperidin, 16.64 min; (2) glycyrrhizin, 29.74 min; (3) atractylenolide III, 37.46 min.
Mentions: The results of HPLC-DAD analysis, using the mobile phases consisting of 0.1% aqueous trifluoroacetic acid (v/v) and pure acetonitrile with a gradient flow, showed satisfactory separation (Table 1). Optimal absorbance for all analytes (hesperidin, 201 nm; glycyrrhizin, 250 nm; atractylenolide III, 222 nm) was detected at 230 nm (Figure 7). Three of the retention time peaks (hesperidin, 16.64 min; glycyrrhizin, 29.74 min; atractylenolide III, 37.46 min) showed chromatograms of the reference components and a 60% methanol extract of PW (Figure 7). These compounds were identified qualitatively by comparing the retention times and UV wavelengths with those of standard compounds.

Bottom Line: However, its effects on inflammation-related diseases are unknown.We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time.Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine (KM)-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong, Daejeon 305-811, Korea. ulivuli@kiom.re.kr.

ABSTRACT
Pyeongwisan (PW) is an herbal medication used in traditional East Asian medicine to treat anorexia, abdominal distension, borborygmus and diarrhea caused by gastric catarrh, atony and dilatation. However, its effects on inflammation-related diseases are unknown. In this study, we investigated the biological effects of PW on lipopolysaccharide (LPS)-mediated inflammation in macrophages and on local inflammation in vivo. We investigated the biological effects of PW on the production of inflammatory mediators, pro-inflammatory cytokines and related products as well as the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated macrophages. Additionally, we evaluated the analgesic effect on the acetic acid-induced writhing response and the inhibitory activity on xylene-induced ear edema in mice. PW showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and interleukin-1β (IL-1β). In addition, PW strongly suppressed inducible nitric oxide synthase (iNOS), a NO synthesis enzyme, induced heme oxygenase-1 (HO-1) expression and inhibited NF-κB activation and MAPK phosphorylation. Also, PW suppressed TNF-α, IL-6 and IL-1β cytokine production in LPS-stimulated peritoneal macrophage cells. Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema. We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time. Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

Show MeSH
Related in: MedlinePlus