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Anti-inflammatory and analgesic effects of pyeongwisan on LPS-stimulated murine macrophages and mouse models of acetic acid-induced writhing response and xylene-induced ear edema.

Oh YC, Jeong YH, Cho WK, Ha JH, Gu MJ, Ma JY - Int J Mol Sci (2015)

Bottom Line: However, its effects on inflammation-related diseases are unknown.We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time.Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine (KM)-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong, Daejeon 305-811, Korea. ulivuli@kiom.re.kr.

ABSTRACT
Pyeongwisan (PW) is an herbal medication used in traditional East Asian medicine to treat anorexia, abdominal distension, borborygmus and diarrhea caused by gastric catarrh, atony and dilatation. However, its effects on inflammation-related diseases are unknown. In this study, we investigated the biological effects of PW on lipopolysaccharide (LPS)-mediated inflammation in macrophages and on local inflammation in vivo. We investigated the biological effects of PW on the production of inflammatory mediators, pro-inflammatory cytokines and related products as well as the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated macrophages. Additionally, we evaluated the analgesic effect on the acetic acid-induced writhing response and the inhibitory activity on xylene-induced ear edema in mice. PW showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and interleukin-1β (IL-1β). In addition, PW strongly suppressed inducible nitric oxide synthase (iNOS), a NO synthesis enzyme, induced heme oxygenase-1 (HO-1) expression and inhibited NF-κB activation and MAPK phosphorylation. Also, PW suppressed TNF-α, IL-6 and IL-1β cytokine production in LPS-stimulated peritoneal macrophage cells. Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema. We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time. Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

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The effects of PW on (A) cyclooxygenase-2 (COX-2), (B) inducible NO synthase (iNOS) and (C,D) heme oxygenase-1 (HO-1) in macrophages. The cells were treated with (A,B) LPS alone or with LPS and PW for 24 h and (C,D) PW alone for the indicated periods. Protein levels were evaluated using Western blot analysis as described in the Materials and Methods and were quantitated using a Davinch-chemi™ Chemiluminescence Imaging System CAS-400SM (Core Bio, Seoul, Korea). The experiment was repeated three times independently and similar results were obtained. Con: control; Dex: Dexamethasone. **p < 0.001 in comparisons of the (A,B) LPS-stimulation value or (D) non-treated control value.
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ijms-16-01232-f002: The effects of PW on (A) cyclooxygenase-2 (COX-2), (B) inducible NO synthase (iNOS) and (C,D) heme oxygenase-1 (HO-1) in macrophages. The cells were treated with (A,B) LPS alone or with LPS and PW for 24 h and (C,D) PW alone for the indicated periods. Protein levels were evaluated using Western blot analysis as described in the Materials and Methods and were quantitated using a Davinch-chemi™ Chemiluminescence Imaging System CAS-400SM (Core Bio, Seoul, Korea). The experiment was repeated three times independently and similar results were obtained. Con: control; Dex: Dexamethasone. **p < 0.001 in comparisons of the (A,B) LPS-stimulation value or (D) non-treated control value.

Mentions: COX-2 and iNOS expression were investigated next using Western blot analysis and RT-PCR. As presented in Figure 2A, PW did not show any suppressive effect on either COX-2 protein or mRNA expression. However, iNOS protein and mRNA expression was significantly inhibited by PW in a concentration-dependent manner (Figure 2B). The inhibition of PW on iNOS expression was closely related to the suppression of NO production.


Anti-inflammatory and analgesic effects of pyeongwisan on LPS-stimulated murine macrophages and mouse models of acetic acid-induced writhing response and xylene-induced ear edema.

Oh YC, Jeong YH, Cho WK, Ha JH, Gu MJ, Ma JY - Int J Mol Sci (2015)

The effects of PW on (A) cyclooxygenase-2 (COX-2), (B) inducible NO synthase (iNOS) and (C,D) heme oxygenase-1 (HO-1) in macrophages. The cells were treated with (A,B) LPS alone or with LPS and PW for 24 h and (C,D) PW alone for the indicated periods. Protein levels were evaluated using Western blot analysis as described in the Materials and Methods and were quantitated using a Davinch-chemi™ Chemiluminescence Imaging System CAS-400SM (Core Bio, Seoul, Korea). The experiment was repeated three times independently and similar results were obtained. Con: control; Dex: Dexamethasone. **p < 0.001 in comparisons of the (A,B) LPS-stimulation value or (D) non-treated control value.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307301&req=5

ijms-16-01232-f002: The effects of PW on (A) cyclooxygenase-2 (COX-2), (B) inducible NO synthase (iNOS) and (C,D) heme oxygenase-1 (HO-1) in macrophages. The cells were treated with (A,B) LPS alone or with LPS and PW for 24 h and (C,D) PW alone for the indicated periods. Protein levels were evaluated using Western blot analysis as described in the Materials and Methods and were quantitated using a Davinch-chemi™ Chemiluminescence Imaging System CAS-400SM (Core Bio, Seoul, Korea). The experiment was repeated three times independently and similar results were obtained. Con: control; Dex: Dexamethasone. **p < 0.001 in comparisons of the (A,B) LPS-stimulation value or (D) non-treated control value.
Mentions: COX-2 and iNOS expression were investigated next using Western blot analysis and RT-PCR. As presented in Figure 2A, PW did not show any suppressive effect on either COX-2 protein or mRNA expression. However, iNOS protein and mRNA expression was significantly inhibited by PW in a concentration-dependent manner (Figure 2B). The inhibition of PW on iNOS expression was closely related to the suppression of NO production.

Bottom Line: However, its effects on inflammation-related diseases are unknown.We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time.Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine (KM)-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong, Daejeon 305-811, Korea. ulivuli@kiom.re.kr.

ABSTRACT
Pyeongwisan (PW) is an herbal medication used in traditional East Asian medicine to treat anorexia, abdominal distension, borborygmus and diarrhea caused by gastric catarrh, atony and dilatation. However, its effects on inflammation-related diseases are unknown. In this study, we investigated the biological effects of PW on lipopolysaccharide (LPS)-mediated inflammation in macrophages and on local inflammation in vivo. We investigated the biological effects of PW on the production of inflammatory mediators, pro-inflammatory cytokines and related products as well as the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated macrophages. Additionally, we evaluated the analgesic effect on the acetic acid-induced writhing response and the inhibitory activity on xylene-induced ear edema in mice. PW showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and interleukin-1β (IL-1β). In addition, PW strongly suppressed inducible nitric oxide synthase (iNOS), a NO synthesis enzyme, induced heme oxygenase-1 (HO-1) expression and inhibited NF-κB activation and MAPK phosphorylation. Also, PW suppressed TNF-α, IL-6 and IL-1β cytokine production in LPS-stimulated peritoneal macrophage cells. Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema. We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time. Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

Show MeSH
Related in: MedlinePlus