Limits...
Anti-inflammatory and analgesic effects of pyeongwisan on LPS-stimulated murine macrophages and mouse models of acetic acid-induced writhing response and xylene-induced ear edema.

Oh YC, Jeong YH, Cho WK, Ha JH, Gu MJ, Ma JY - Int J Mol Sci (2015)

Bottom Line: However, its effects on inflammation-related diseases are unknown.Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema.Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine (KM)-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong, Daejeon 305-811, Korea. ulivuli@kiom.re.kr.

ABSTRACT
Pyeongwisan (PW) is an herbal medication used in traditional East Asian medicine to treat anorexia, abdominal distension, borborygmus and diarrhea caused by gastric catarrh, atony and dilatation. However, its effects on inflammation-related diseases are unknown. In this study, we investigated the biological effects of PW on lipopolysaccharide (LPS)-mediated inflammation in macrophages and on local inflammation in vivo. We investigated the biological effects of PW on the production of inflammatory mediators, pro-inflammatory cytokines and related products as well as the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated macrophages. Additionally, we evaluated the analgesic effect on the acetic acid-induced writhing response and the inhibitory activity on xylene-induced ear edema in mice. PW showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and interleukin-1β (IL-1β). In addition, PW strongly suppressed inducible nitric oxide synthase (iNOS), a NO synthesis enzyme, induced heme oxygenase-1 (HO-1) expression and inhibited NF-κB activation and MAPK phosphorylation. Also, PW suppressed TNF-α, IL-6 and IL-1β cytokine production in LPS-stimulated peritoneal macrophage cells. Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema. We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time. Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

Show MeSH

Related in: MedlinePlus

(A) Pyeongwisan (PW) cytotoxicity and (B–F) suppressive effect of PW on NO and cytokine production. RAW 264.7 cells were pretreated with PW for 30 min before incubation with LPS for (A–E) 24 h or (F) 6 h. (A) The cytotoxicity was determined using cell-counting kit (CCK); (B) The culture supernatant was analyzed for nitrite production; (C–E) Production of cytokines was measured using ELISA; and (F) mRNA levels were analyzed by RT-PCR. RNA values were quantitated using an i-MAX™ Gel Image Analysis System (Core Bio, Seoul, Korea). As a control, the cells were incubated with vehicle alone. Data represent means ± SE of duplicate determinations from three independent experiments. Con: control; Dex: Dexamethasone. *p < 0.01 and **p < 0.001 in comparisons of the LPS-stimulation value.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4307301&req=5

ijms-16-01232-f001: (A) Pyeongwisan (PW) cytotoxicity and (B–F) suppressive effect of PW on NO and cytokine production. RAW 264.7 cells were pretreated with PW for 30 min before incubation with LPS for (A–E) 24 h or (F) 6 h. (A) The cytotoxicity was determined using cell-counting kit (CCK); (B) The culture supernatant was analyzed for nitrite production; (C–E) Production of cytokines was measured using ELISA; and (F) mRNA levels were analyzed by RT-PCR. RNA values were quantitated using an i-MAX™ Gel Image Analysis System (Core Bio, Seoul, Korea). As a control, the cells were incubated with vehicle alone. Data represent means ± SE of duplicate determinations from three independent experiments. Con: control; Dex: Dexamethasone. *p < 0.01 and **p < 0.001 in comparisons of the LPS-stimulation value.

Mentions: In this study, we evaluated the anti-inflammatory and analgesic activities of PW in macrophages and a mouse model. We first examined PW cytotoxicity at concentrations of 10–1000 μg/mL in macrophages. As shown in Figure 1A, PW was not cytotoxic even at 1000 μg/mL, indicating no toxicity in macrophages.


Anti-inflammatory and analgesic effects of pyeongwisan on LPS-stimulated murine macrophages and mouse models of acetic acid-induced writhing response and xylene-induced ear edema.

Oh YC, Jeong YH, Cho WK, Ha JH, Gu MJ, Ma JY - Int J Mol Sci (2015)

(A) Pyeongwisan (PW) cytotoxicity and (B–F) suppressive effect of PW on NO and cytokine production. RAW 264.7 cells were pretreated with PW for 30 min before incubation with LPS for (A–E) 24 h or (F) 6 h. (A) The cytotoxicity was determined using cell-counting kit (CCK); (B) The culture supernatant was analyzed for nitrite production; (C–E) Production of cytokines was measured using ELISA; and (F) mRNA levels were analyzed by RT-PCR. RNA values were quantitated using an i-MAX™ Gel Image Analysis System (Core Bio, Seoul, Korea). As a control, the cells were incubated with vehicle alone. Data represent means ± SE of duplicate determinations from three independent experiments. Con: control; Dex: Dexamethasone. *p < 0.01 and **p < 0.001 in comparisons of the LPS-stimulation value.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307301&req=5

ijms-16-01232-f001: (A) Pyeongwisan (PW) cytotoxicity and (B–F) suppressive effect of PW on NO and cytokine production. RAW 264.7 cells were pretreated with PW for 30 min before incubation with LPS for (A–E) 24 h or (F) 6 h. (A) The cytotoxicity was determined using cell-counting kit (CCK); (B) The culture supernatant was analyzed for nitrite production; (C–E) Production of cytokines was measured using ELISA; and (F) mRNA levels were analyzed by RT-PCR. RNA values were quantitated using an i-MAX™ Gel Image Analysis System (Core Bio, Seoul, Korea). As a control, the cells were incubated with vehicle alone. Data represent means ± SE of duplicate determinations from three independent experiments. Con: control; Dex: Dexamethasone. *p < 0.01 and **p < 0.001 in comparisons of the LPS-stimulation value.
Mentions: In this study, we evaluated the anti-inflammatory and analgesic activities of PW in macrophages and a mouse model. We first examined PW cytotoxicity at concentrations of 10–1000 μg/mL in macrophages. As shown in Figure 1A, PW was not cytotoxic even at 1000 μg/mL, indicating no toxicity in macrophages.

Bottom Line: However, its effects on inflammation-related diseases are unknown.Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema.Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

View Article: PubMed Central - PubMed

Affiliation: Korean Medicine (KM)-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong, Daejeon 305-811, Korea. ulivuli@kiom.re.kr.

ABSTRACT
Pyeongwisan (PW) is an herbal medication used in traditional East Asian medicine to treat anorexia, abdominal distension, borborygmus and diarrhea caused by gastric catarrh, atony and dilatation. However, its effects on inflammation-related diseases are unknown. In this study, we investigated the biological effects of PW on lipopolysaccharide (LPS)-mediated inflammation in macrophages and on local inflammation in vivo. We investigated the biological effects of PW on the production of inflammatory mediators, pro-inflammatory cytokines and related products as well as the activation of nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated macrophages. Additionally, we evaluated the analgesic effect on the acetic acid-induced writhing response and the inhibitory activity on xylene-induced ear edema in mice. PW showed anti-inflammatory effects by inhibiting the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and interleukin-1β (IL-1β). In addition, PW strongly suppressed inducible nitric oxide synthase (iNOS), a NO synthesis enzyme, induced heme oxygenase-1 (HO-1) expression and inhibited NF-κB activation and MAPK phosphorylation. Also, PW suppressed TNF-α, IL-6 and IL-1β cytokine production in LPS-stimulated peritoneal macrophage cells. Furthermore, PW showed an analgesic effect on the writhing response and an inhibitory effect on mice ear edema. We demonstrated the anti-inflammatory effects and inhibitory mechanism in macrophages as well as inhibitory activity of PW in vivo for the first time. Our results suggest the potential value of PW as an inflammatory therapeutic agent developed from a natural substance.

Show MeSH
Related in: MedlinePlus