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Identification of retinopathy of prematurity related miRNAs in hyperoxia-induced neonatal rats by deep sequencing.

Zhao R, Qian L, Jiang L - Int J Mol Sci (2014)

Bottom Line: Some of the differentially expressed miRNAs were confirmed by quantitative reverse transcription-PCR (qRT-PCR).A total of 594 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach.This study provides some insights into the molecular mechanisms that underlie ROP development, thereby aiding the diagnosis and treatment of this disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing 210096, China. tczhaoruibin@163.com.

ABSTRACT
Retinopathy of prematurity (ROP) remains a major problem for many preterm infants. MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the posttranscriptional level and have been studied in many diseases. To understand the roles of miRNAs in ROP model rats, we constructed two small RNA libraries from the plasma of hyperoxia-induced rats and normal controls. Sequencing data revealed that 44 down-regulated microRNAs and 22 up-regulated microRNAs from the hyperoxia-induced rats were identified by deep sequencing technology. Some of the differentially expressed miRNAs were confirmed by quantitative reverse transcription-PCR (qRT-PCR). A total of 594 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach. Functional annotation analysis indicated that a number of pathways might be involved in angiogenesis, cell proliferation and cell differentiation, which might be involved in the genesis and development of ROP. The elevated expression level of the vascular endothelial growth factor (VEGF) protein in the hyperoxia-induced neonatal rats was also confirmed by enzyme linked immunosorbent assay (ELISA). This study provides some insights into the molecular mechanisms that underlie ROP development, thereby aiding the diagnosis and treatment of this disease.

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(a) The level of vascular endothelial growth factor (VEGF) protein secretion into the plasma of the hyperoxia model rats and controls were evaluated with enzyme-linked immunosorbent assay (ELISA) (mean ± SEM; *p < 0.01); and (b) Network interactions of the dysregulated miRNAs and the target gene VEGF.
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ijms-16-00840-f005: (a) The level of vascular endothelial growth factor (VEGF) protein secretion into the plasma of the hyperoxia model rats and controls were evaluated with enzyme-linked immunosorbent assay (ELISA) (mean ± SEM; *p < 0.01); and (b) Network interactions of the dysregulated miRNAs and the target gene VEGF.

Mentions: VEGF was listed among the target genes of the differential miRNAs that were differentially expressed in the hyperoxia model rats in this study. The level of VEGF secretion into the plasma was evaluated with an enzyme-linked immunosorbent assay (ELISA). As shown in Figure 5a, the VEGF concentration in the hyperoxia group was significantly increased compared to that of the control group (p < 0.01). The interaction network of VEGF and its related miRNAs indicated that differential expressions were also demonstrated in this study. As shown in Figure 5b, VEGFA were targeted by the greatest number of the differential miRNAs, among which miR-429, miR-200b and miR-200c also interacted with VEGFC; in contrast, VEGFB was targeted only by miR-18a, miR-326, miR-330 and miR-128. The different colors of the miRNAs indicate dysregulation in the hyperoxia model rats; red indicates up-regulation, and green denotes down-regulation.


Identification of retinopathy of prematurity related miRNAs in hyperoxia-induced neonatal rats by deep sequencing.

Zhao R, Qian L, Jiang L - Int J Mol Sci (2014)

(a) The level of vascular endothelial growth factor (VEGF) protein secretion into the plasma of the hyperoxia model rats and controls were evaluated with enzyme-linked immunosorbent assay (ELISA) (mean ± SEM; *p < 0.01); and (b) Network interactions of the dysregulated miRNAs and the target gene VEGF.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307278&req=5

ijms-16-00840-f005: (a) The level of vascular endothelial growth factor (VEGF) protein secretion into the plasma of the hyperoxia model rats and controls were evaluated with enzyme-linked immunosorbent assay (ELISA) (mean ± SEM; *p < 0.01); and (b) Network interactions of the dysregulated miRNAs and the target gene VEGF.
Mentions: VEGF was listed among the target genes of the differential miRNAs that were differentially expressed in the hyperoxia model rats in this study. The level of VEGF secretion into the plasma was evaluated with an enzyme-linked immunosorbent assay (ELISA). As shown in Figure 5a, the VEGF concentration in the hyperoxia group was significantly increased compared to that of the control group (p < 0.01). The interaction network of VEGF and its related miRNAs indicated that differential expressions were also demonstrated in this study. As shown in Figure 5b, VEGFA were targeted by the greatest number of the differential miRNAs, among which miR-429, miR-200b and miR-200c also interacted with VEGFC; in contrast, VEGFB was targeted only by miR-18a, miR-326, miR-330 and miR-128. The different colors of the miRNAs indicate dysregulation in the hyperoxia model rats; red indicates up-regulation, and green denotes down-regulation.

Bottom Line: Some of the differentially expressed miRNAs were confirmed by quantitative reverse transcription-PCR (qRT-PCR).A total of 594 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach.This study provides some insights into the molecular mechanisms that underlie ROP development, thereby aiding the diagnosis and treatment of this disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Zhongda Hospital, Southeast University, Nanjing 210096, China. tczhaoruibin@163.com.

ABSTRACT
Retinopathy of prematurity (ROP) remains a major problem for many preterm infants. MicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression at the posttranscriptional level and have been studied in many diseases. To understand the roles of miRNAs in ROP model rats, we constructed two small RNA libraries from the plasma of hyperoxia-induced rats and normal controls. Sequencing data revealed that 44 down-regulated microRNAs and 22 up-regulated microRNAs from the hyperoxia-induced rats were identified by deep sequencing technology. Some of the differentially expressed miRNAs were confirmed by quantitative reverse transcription-PCR (qRT-PCR). A total of 594 target genes of the differentially expressed microRNAs were identified using a bioinformatics approach. Functional annotation analysis indicated that a number of pathways might be involved in angiogenesis, cell proliferation and cell differentiation, which might be involved in the genesis and development of ROP. The elevated expression level of the vascular endothelial growth factor (VEGF) protein in the hyperoxia-induced neonatal rats was also confirmed by enzyme linked immunosorbent assay (ELISA). This study provides some insights into the molecular mechanisms that underlie ROP development, thereby aiding the diagnosis and treatment of this disease.

Show MeSH
Related in: MedlinePlus