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Morin, a flavonoid from moraceae, induces apoptosis by induction of BAD protein in human leukemic cells.

Park C, Lee WS, Go SI, Nagappan A, Han MH, Hong SH, Kim GS, Kim GY, Kwon TK, Ryu CH, Shin SC, Choi YH - Int J Mol Sci (2014)

Bottom Line: An anti-cancer effect of morin was screened with several human leukemic cell lines.In conclusion, morin induced caspase-dependent apoptosis through an intrinsic pathway by upregulating BAD proteins.This study provides evidence that morin might have anticancer properties in human leukemic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan 614-714, Korea. parkch@deu.ac.kr.

ABSTRACT
Evidence suggests that phytochemicals can safely modulate cancer cell biology and induce apoptosis. Here, we investigated the anti-cancer activity of morin, a flavone originally isolated from members of the Moraceae family in human leukemic cells, focusing on apoptosis. An anti-cancer effect of morin was screened with several human leukemic cell lines. U937 cells were most sensitive to morin, where it induced caspase-dependent apoptosis in a dose-dependent manner. It also induced loss of MMP (ΔΨm) along with cytochrome c release, down-regulated Bcl-2 protein, and up-regulated BAX proteins. The apoptotic activity of morin was significantly attenuated by Bcl-2 augmentation. In conclusion, morin induced caspase-dependent apoptosis through an intrinsic pathway by upregulating BAD proteins. In addition, Bcl-2 protein expression is also important in morin-induced apoptosis of U937 cells. This study provides evidence that morin might have anticancer properties in human leukemic cells.

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Proposed model of morin mechanism of action for apoptosis in U937 human leukemic cells. Morin induced caspase-dependent apoptosis; induced loss of MMP (ΔΨm) along with cytochrome c release, downregulated Bcl-2 protein, and upregulated BAX proteins. It activates caspase-3 and caspase-9, and subsequent cleavage of PARP. In addition, caspase-3, z-DEVD-fmk reduced morin-induced cell death. Taken together, this study suggests that morin induces caspase-dependent apoptosis through an intrinsic pathway by modulating Bcl-2 family members, BAD and Bcl-xL, which regulates the apoptotic effect of morin in human leukemic cells.
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ijms-16-00645-f006: Proposed model of morin mechanism of action for apoptosis in U937 human leukemic cells. Morin induced caspase-dependent apoptosis; induced loss of MMP (ΔΨm) along with cytochrome c release, downregulated Bcl-2 protein, and upregulated BAX proteins. It activates caspase-3 and caspase-9, and subsequent cleavage of PARP. In addition, caspase-3, z-DEVD-fmk reduced morin-induced cell death. Taken together, this study suggests that morin induces caspase-dependent apoptosis through an intrinsic pathway by modulating Bcl-2 family members, BAD and Bcl-xL, which regulates the apoptotic effect of morin in human leukemic cells.

Mentions: In conclusion, morin showed anti-cancer effects in several leukemic cell lines. The cytotoxicity may result from apoptosis through the mitochondrial pathway by Bcl-2 inhibition and activation of BAD protein (Figure 6). This study provides evidence that morin might be useful for the treatment of human hematopoietic cancer cells.


Morin, a flavonoid from moraceae, induces apoptosis by induction of BAD protein in human leukemic cells.

Park C, Lee WS, Go SI, Nagappan A, Han MH, Hong SH, Kim GS, Kim GY, Kwon TK, Ryu CH, Shin SC, Choi YH - Int J Mol Sci (2014)

Proposed model of morin mechanism of action for apoptosis in U937 human leukemic cells. Morin induced caspase-dependent apoptosis; induced loss of MMP (ΔΨm) along with cytochrome c release, downregulated Bcl-2 protein, and upregulated BAX proteins. It activates caspase-3 and caspase-9, and subsequent cleavage of PARP. In addition, caspase-3, z-DEVD-fmk reduced morin-induced cell death. Taken together, this study suggests that morin induces caspase-dependent apoptosis through an intrinsic pathway by modulating Bcl-2 family members, BAD and Bcl-xL, which regulates the apoptotic effect of morin in human leukemic cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307266&req=5

ijms-16-00645-f006: Proposed model of morin mechanism of action for apoptosis in U937 human leukemic cells. Morin induced caspase-dependent apoptosis; induced loss of MMP (ΔΨm) along with cytochrome c release, downregulated Bcl-2 protein, and upregulated BAX proteins. It activates caspase-3 and caspase-9, and subsequent cleavage of PARP. In addition, caspase-3, z-DEVD-fmk reduced morin-induced cell death. Taken together, this study suggests that morin induces caspase-dependent apoptosis through an intrinsic pathway by modulating Bcl-2 family members, BAD and Bcl-xL, which regulates the apoptotic effect of morin in human leukemic cells.
Mentions: In conclusion, morin showed anti-cancer effects in several leukemic cell lines. The cytotoxicity may result from apoptosis through the mitochondrial pathway by Bcl-2 inhibition and activation of BAD protein (Figure 6). This study provides evidence that morin might be useful for the treatment of human hematopoietic cancer cells.

Bottom Line: An anti-cancer effect of morin was screened with several human leukemic cell lines.In conclusion, morin induced caspase-dependent apoptosis through an intrinsic pathway by upregulating BAD proteins.This study provides evidence that morin might have anticancer properties in human leukemic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan 614-714, Korea. parkch@deu.ac.kr.

ABSTRACT
Evidence suggests that phytochemicals can safely modulate cancer cell biology and induce apoptosis. Here, we investigated the anti-cancer activity of morin, a flavone originally isolated from members of the Moraceae family in human leukemic cells, focusing on apoptosis. An anti-cancer effect of morin was screened with several human leukemic cell lines. U937 cells were most sensitive to morin, where it induced caspase-dependent apoptosis in a dose-dependent manner. It also induced loss of MMP (ΔΨm) along with cytochrome c release, down-regulated Bcl-2 protein, and up-regulated BAX proteins. The apoptotic activity of morin was significantly attenuated by Bcl-2 augmentation. In conclusion, morin induced caspase-dependent apoptosis through an intrinsic pathway by upregulating BAD proteins. In addition, Bcl-2 protein expression is also important in morin-induced apoptosis of U937 cells. This study provides evidence that morin might have anticancer properties in human leukemic cells.

Show MeSH
Related in: MedlinePlus