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Accelerating in situ endothelialisation of cardiovascular bypass grafts.

Goh ET, Wong E, Farhatnia Y, Tan A, Seifalian AM - Int J Mol Sci (2014)

Bottom Line: This can be done either by seeding the endothelium within the lumen of the grafts prior to implantation or by designing the graft such that in situ endothelialisation takes place after implantation.Due to certain difficulties identified with in vitro endothelialisation, in situ endothelialisation, which will be the focus of this article, has garnered interest in the last years.To promote in situ endothelialisation, the following aspects can be taken into account: (1) Endothelial progenital cell mobilization, adhesion and proliferation; (2) Regulating differentiation of progenitor cells to mature endothelium; (3) Preventing thrombogenesis and inflammation during endothelialisation.

View Article: PubMed Central - PubMed

Affiliation: Centre for Nanotechnology & Regenerative Medicine, Research Department of Nanotechnology, UCL Division of Surgery & Interventional Science, University College London (UCL), London NW3 2QG, UK. ee.goh.12@ucl.ac.uk.

ABSTRACT
The patency of synthetic cardiovascular grafts in the long run is synonymous with their ability to inhibit the processes of intimal hyperplasia, thrombosis and calcification. In the human body, the endothelium of blood vessels exhibits characteristics that inhibit such processes. As such it is not surprising that research in tissue engineering is directed towards replicating the functionality of the natural endothelium in cardiovascular grafts. This can be done either by seeding the endothelium within the lumen of the grafts prior to implantation or by designing the graft such that in situ endothelialisation takes place after implantation. Due to certain difficulties identified with in vitro endothelialisation, in situ endothelialisation, which will be the focus of this article, has garnered interest in the last years. To promote in situ endothelialisation, the following aspects can be taken into account: (1) Endothelial progenital cell mobilization, adhesion and proliferation; (2) Regulating differentiation of progenitor cells to mature endothelium; (3) Preventing thrombogenesis and inflammation during endothelialisation. This article aims to review and compile recent developments to promote the in situ endothelialisation of cardiovascular grafts and subsequently improve their patency, which can also have widespread implications in the field of tissue engineering.

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Related in: MedlinePlus

Summarizes the basic principles of photolithography and electron beam lithography [118]. Reproduced form [118] with permission from Elsevier, copyright 2014.
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ijms-16-00597-f004: Summarizes the basic principles of photolithography and electron beam lithography [118]. Reproduced form [118] with permission from Elsevier, copyright 2014.

Mentions: In photolithography, light is used to generate desired patterns on a surface. A light-sensitive polymer (a photoresist) is exposed to UV light through a mask layout. This causes crosslinking, polymerisation or degradation of the photoresist and 2-dimensional (2D) features can be constructed. EBL is an extension of photolithography which uses high-energy electrons instead to create patterns on a nanoscale, unlike photolithography which is used to develop microscale surfaces [122]. Figure 4 below briefly explains the processes of photolithography and EBL.


Accelerating in situ endothelialisation of cardiovascular bypass grafts.

Goh ET, Wong E, Farhatnia Y, Tan A, Seifalian AM - Int J Mol Sci (2014)

Summarizes the basic principles of photolithography and electron beam lithography [118]. Reproduced form [118] with permission from Elsevier, copyright 2014.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307264&req=5

ijms-16-00597-f004: Summarizes the basic principles of photolithography and electron beam lithography [118]. Reproduced form [118] with permission from Elsevier, copyright 2014.
Mentions: In photolithography, light is used to generate desired patterns on a surface. A light-sensitive polymer (a photoresist) is exposed to UV light through a mask layout. This causes crosslinking, polymerisation or degradation of the photoresist and 2-dimensional (2D) features can be constructed. EBL is an extension of photolithography which uses high-energy electrons instead to create patterns on a nanoscale, unlike photolithography which is used to develop microscale surfaces [122]. Figure 4 below briefly explains the processes of photolithography and EBL.

Bottom Line: This can be done either by seeding the endothelium within the lumen of the grafts prior to implantation or by designing the graft such that in situ endothelialisation takes place after implantation.Due to certain difficulties identified with in vitro endothelialisation, in situ endothelialisation, which will be the focus of this article, has garnered interest in the last years.To promote in situ endothelialisation, the following aspects can be taken into account: (1) Endothelial progenital cell mobilization, adhesion and proliferation; (2) Regulating differentiation of progenitor cells to mature endothelium; (3) Preventing thrombogenesis and inflammation during endothelialisation.

View Article: PubMed Central - PubMed

Affiliation: Centre for Nanotechnology & Regenerative Medicine, Research Department of Nanotechnology, UCL Division of Surgery & Interventional Science, University College London (UCL), London NW3 2QG, UK. ee.goh.12@ucl.ac.uk.

ABSTRACT
The patency of synthetic cardiovascular grafts in the long run is synonymous with their ability to inhibit the processes of intimal hyperplasia, thrombosis and calcification. In the human body, the endothelium of blood vessels exhibits characteristics that inhibit such processes. As such it is not surprising that research in tissue engineering is directed towards replicating the functionality of the natural endothelium in cardiovascular grafts. This can be done either by seeding the endothelium within the lumen of the grafts prior to implantation or by designing the graft such that in situ endothelialisation takes place after implantation. Due to certain difficulties identified with in vitro endothelialisation, in situ endothelialisation, which will be the focus of this article, has garnered interest in the last years. To promote in situ endothelialisation, the following aspects can be taken into account: (1) Endothelial progenital cell mobilization, adhesion and proliferation; (2) Regulating differentiation of progenitor cells to mature endothelium; (3) Preventing thrombogenesis and inflammation during endothelialisation. This article aims to review and compile recent developments to promote the in situ endothelialisation of cardiovascular grafts and subsequently improve their patency, which can also have widespread implications in the field of tissue engineering.

Show MeSH
Related in: MedlinePlus