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Cyclodextrin-complexed Ocimum basilicum leaves essential oil increases Fos protein expression in the central nervous system and produce an antihyperalgesic effect in animal models for fibromyalgia.

Nascimento SS, Araújo AA, Brito RG, Serafini MR, Menezes PP, DeSantana JM, Lucca W, Alves PB, Blank AF, Oliveira RC, Oliveira AP, Albuquerque RL, Almeida JR, Quintans LJ - Int J Mol Sci (2014)

Bottom Line: After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein.Oral treatment with LEO or LEO-βCD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression.Together, our results provide evidence that LEO, isolated or complexed with β-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology, Federal University of Sergipe, Av. Tancredo Neves, S/N, Rosa Elza, CEP: 49.000-100, São Cristóvão, Sergipe 49.100-000, Brazil. simonenascimento.saude@gmail.com.

ABSTRACT
O. basilicum leaves produce essential oils (LEO) rich in monoterpenes. The short half-life and water insolubility are limitations for LEO medical uses. β-Cyclodextrin (β-CD) has been employed to improve the pharmacological properties of LEO. We assessed the antihyperalgesic profile of LEO, isolated or complexed in β-CD (LEO/β-CD), on an animal model for fibromyalgia. Behavioral tests: mice were treated every day with either LEO/β-CD (25, 50 or 100 mg/kg, p.o.), LEO (25 mg/kg, p.o.), tramadol (TRM 4 mg/kg, i.p.) or vehicle (saline), and 60 min after treatment behavioral parameters were assessed. Therefore, mice were evaluated for mechanical hyperalgesia (von Frey), motor coordination (Rota-rod) and muscle strength (Grip Strength Metter) in a mice fibromyalgia model. After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein. The differential scanning analysis (DSC), thermogravimetry/derivate thermogravimetry (TG/DTG) and infrared absorption spectroscopy (FTIR) curves indicated that the products prepared were able to incorporate the LEO efficiently. Oral treatment with LEO or LEO-βCD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression. Together, our results provide evidence that LEO, isolated or complexed with β-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.

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Related in: MedlinePlus

TG/DTG curves of the essential oil of Ocimum basilicum (LEO), β-cyclodextrin (β-CD), physical mixture (PM), paste complex (PC) and co-evaporation (EC) obtained in a dynamic atmosphere of N2.
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ijms-16-00547-f002: TG/DTG curves of the essential oil of Ocimum basilicum (LEO), β-cyclodextrin (β-CD), physical mixture (PM), paste complex (PC) and co-evaporation (EC) obtained in a dynamic atmosphere of N2.

Mentions: As it can be seen in Figure 2, the results of physicochemical characterization DSC and TG/DTG curves indicated that the products prepared were able to incorporate the LEO efficiently by the co-evaporation technique, indicating the presence of the compound in the complex, what can increase the water solubility of the LEO (Figure 2).


Cyclodextrin-complexed Ocimum basilicum leaves essential oil increases Fos protein expression in the central nervous system and produce an antihyperalgesic effect in animal models for fibromyalgia.

Nascimento SS, Araújo AA, Brito RG, Serafini MR, Menezes PP, DeSantana JM, Lucca W, Alves PB, Blank AF, Oliveira RC, Oliveira AP, Albuquerque RL, Almeida JR, Quintans LJ - Int J Mol Sci (2014)

TG/DTG curves of the essential oil of Ocimum basilicum (LEO), β-cyclodextrin (β-CD), physical mixture (PM), paste complex (PC) and co-evaporation (EC) obtained in a dynamic atmosphere of N2.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307262&req=5

ijms-16-00547-f002: TG/DTG curves of the essential oil of Ocimum basilicum (LEO), β-cyclodextrin (β-CD), physical mixture (PM), paste complex (PC) and co-evaporation (EC) obtained in a dynamic atmosphere of N2.
Mentions: As it can be seen in Figure 2, the results of physicochemical characterization DSC and TG/DTG curves indicated that the products prepared were able to incorporate the LEO efficiently by the co-evaporation technique, indicating the presence of the compound in the complex, what can increase the water solubility of the LEO (Figure 2).

Bottom Line: After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein.Oral treatment with LEO or LEO-βCD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression.Together, our results provide evidence that LEO, isolated or complexed with β-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology, Federal University of Sergipe, Av. Tancredo Neves, S/N, Rosa Elza, CEP: 49.000-100, São Cristóvão, Sergipe 49.100-000, Brazil. simonenascimento.saude@gmail.com.

ABSTRACT
O. basilicum leaves produce essential oils (LEO) rich in monoterpenes. The short half-life and water insolubility are limitations for LEO medical uses. β-Cyclodextrin (β-CD) has been employed to improve the pharmacological properties of LEO. We assessed the antihyperalgesic profile of LEO, isolated or complexed in β-CD (LEO/β-CD), on an animal model for fibromyalgia. Behavioral tests: mice were treated every day with either LEO/β-CD (25, 50 or 100 mg/kg, p.o.), LEO (25 mg/kg, p.o.), tramadol (TRM 4 mg/kg, i.p.) or vehicle (saline), and 60 min after treatment behavioral parameters were assessed. Therefore, mice were evaluated for mechanical hyperalgesia (von Frey), motor coordination (Rota-rod) and muscle strength (Grip Strength Metter) in a mice fibromyalgia model. After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein. The differential scanning analysis (DSC), thermogravimetry/derivate thermogravimetry (TG/DTG) and infrared absorption spectroscopy (FTIR) curves indicated that the products prepared were able to incorporate the LEO efficiently. Oral treatment with LEO or LEO-βCD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression. Together, our results provide evidence that LEO, isolated or complexed with β-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.

Show MeSH
Related in: MedlinePlus