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High levels of KAP1 expression are associated with aggressive clinical features in ovarian cancer.

Cui Y, Yang S, Fu X, Feng J, Xu S, Ying G - Int J Mol Sci (2014)

Bottom Line: Kaplan-Meier analysis and Cox proportional hazard modeling were used to assess overall survival to analyze the effect of KAP1 expression on the prognosis of ovarian cancer.KAP1 expression correlated significantly with clinical stage (χ2 = 14.57, p < 0.0001), pathological grade (χ2 = 6.06, p = 0.048) and metastases (χ2 =10.38, p = 0.001).High KAP1 expression was a prognostic factor of ovarian cancer.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cancer Cell Biology, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China. cuiyanfen@163.com.

ABSTRACT
KAP1 is an universal corepressor for Kruppel-associated box zinc finger proteins in both normal and tumor cells. In this study, the biological function and clinical significance of KAP1 expression in ovarian cancer were investigated. Immunohistological staining of KAP1 was evaluated in 111 patients with ovarian epithelial cancer, 15 with ovarian borderline tumor, and 20 normal ovarian tissue. The correlations of KAP1 expression with clinicopathological features were studied. Kaplan-Meier analysis and Cox proportional hazard modeling were used to assess overall survival to analyze the effect of KAP1 expression on the prognosis of ovarian cancer. The positive rates of KAP1 were significantly higher in ovarian epithelial cancer (55.7%) and borderline tumor (20.0%) than in normal ovarian tissue (5.0%) (all p < 0.01). KAP1 expression correlated significantly with clinical stage (χ2 = 14.57, p < 0.0001), pathological grade (χ2 = 6.06, p = 0.048) and metastases (χ2 =10.38, p = 0.001). Patients with high KAP 1 levels showed poor survival (p < 0.0001). Multivariate analysis showed that KAP1 high expression was an independent predictor for ovarian cancer patients (hazard ratio = 0.463; 95% confidence interval = 0.230-0.9318, p = 0.031). Functionally, depletion of KAP1 by siRNA inhibited ovarian cancer cell proliferation, cell migration. KAP1 expression correlated with aggressive clinical features in ovarian cancer. High KAP1 expression was a prognostic factor of ovarian cancer.

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Expression of KAP1 in human non-tumor ovarian tissues and ovarian cancer. (A) in normal ovarian tissue; (B) in borderline tumor; (C) in ovarian cancer; (D) KAP1 expression levels in normal ovarian, borderline tumor and ovarian cancer tissues; (E) the expression level of KAP1 in non-tumor cells was lower than tumor cells in one sample and (F) western blot showed the expression level of KAP1 in cancer tissues was higher than normal ovarian tissues. (IHC, 400×). (**p < 0.01; ***p < 0.001).
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ijms-16-00363-f001: Expression of KAP1 in human non-tumor ovarian tissues and ovarian cancer. (A) in normal ovarian tissue; (B) in borderline tumor; (C) in ovarian cancer; (D) KAP1 expression levels in normal ovarian, borderline tumor and ovarian cancer tissues; (E) the expression level of KAP1 in non-tumor cells was lower than tumor cells in one sample and (F) western blot showed the expression level of KAP1 in cancer tissues was higher than normal ovarian tissues. (IHC, 400×). (**p < 0.01; ***p < 0.001).

Mentions: To investigate the expression of KAP1 in ovarian cancer tissues and normal ovarian tissues, immunohistochemistry (IHC) was applied to show that KAP1 was expressed in both tumor cells and mesenchymal cells, and localized to the cell nucleus (Figure 1). Furthermore, we compared the expression level of KAP1 in 111 patients with ovarian epithelial cancer, 15 with ovarian borderline tumor, and 20 normal ovarian tissue. The results showed that the expression level of KAP1 was higher in ovarian cancer samples than non-tumor ovarian tissues (Figure 1A–D), and the positive rates of KAP1 were significantly higher in ovarian epithelial cancer (55.7%) and borderline tumor (20.0%) than in normal ovarian tissue (5.0%) (all p < 0.01) (Table 1). It also showed that the level of KAP1 in cancer tissues was higher than adjacent normal tissues in the same ovarian cancer sample (Figure 1E).


High levels of KAP1 expression are associated with aggressive clinical features in ovarian cancer.

Cui Y, Yang S, Fu X, Feng J, Xu S, Ying G - Int J Mol Sci (2014)

Expression of KAP1 in human non-tumor ovarian tissues and ovarian cancer. (A) in normal ovarian tissue; (B) in borderline tumor; (C) in ovarian cancer; (D) KAP1 expression levels in normal ovarian, borderline tumor and ovarian cancer tissues; (E) the expression level of KAP1 in non-tumor cells was lower than tumor cells in one sample and (F) western blot showed the expression level of KAP1 in cancer tissues was higher than normal ovarian tissues. (IHC, 400×). (**p < 0.01; ***p < 0.001).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4307251&req=5

ijms-16-00363-f001: Expression of KAP1 in human non-tumor ovarian tissues and ovarian cancer. (A) in normal ovarian tissue; (B) in borderline tumor; (C) in ovarian cancer; (D) KAP1 expression levels in normal ovarian, borderline tumor and ovarian cancer tissues; (E) the expression level of KAP1 in non-tumor cells was lower than tumor cells in one sample and (F) western blot showed the expression level of KAP1 in cancer tissues was higher than normal ovarian tissues. (IHC, 400×). (**p < 0.01; ***p < 0.001).
Mentions: To investigate the expression of KAP1 in ovarian cancer tissues and normal ovarian tissues, immunohistochemistry (IHC) was applied to show that KAP1 was expressed in both tumor cells and mesenchymal cells, and localized to the cell nucleus (Figure 1). Furthermore, we compared the expression level of KAP1 in 111 patients with ovarian epithelial cancer, 15 with ovarian borderline tumor, and 20 normal ovarian tissue. The results showed that the expression level of KAP1 was higher in ovarian cancer samples than non-tumor ovarian tissues (Figure 1A–D), and the positive rates of KAP1 were significantly higher in ovarian epithelial cancer (55.7%) and borderline tumor (20.0%) than in normal ovarian tissue (5.0%) (all p < 0.01) (Table 1). It also showed that the level of KAP1 in cancer tissues was higher than adjacent normal tissues in the same ovarian cancer sample (Figure 1E).

Bottom Line: Kaplan-Meier analysis and Cox proportional hazard modeling were used to assess overall survival to analyze the effect of KAP1 expression on the prognosis of ovarian cancer.KAP1 expression correlated significantly with clinical stage (χ2 = 14.57, p < 0.0001), pathological grade (χ2 = 6.06, p = 0.048) and metastases (χ2 =10.38, p = 0.001).High KAP1 expression was a prognostic factor of ovarian cancer.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cancer Cell Biology, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China. cuiyanfen@163.com.

ABSTRACT
KAP1 is an universal corepressor for Kruppel-associated box zinc finger proteins in both normal and tumor cells. In this study, the biological function and clinical significance of KAP1 expression in ovarian cancer were investigated. Immunohistological staining of KAP1 was evaluated in 111 patients with ovarian epithelial cancer, 15 with ovarian borderline tumor, and 20 normal ovarian tissue. The correlations of KAP1 expression with clinicopathological features were studied. Kaplan-Meier analysis and Cox proportional hazard modeling were used to assess overall survival to analyze the effect of KAP1 expression on the prognosis of ovarian cancer. The positive rates of KAP1 were significantly higher in ovarian epithelial cancer (55.7%) and borderline tumor (20.0%) than in normal ovarian tissue (5.0%) (all p < 0.01). KAP1 expression correlated significantly with clinical stage (χ2 = 14.57, p < 0.0001), pathological grade (χ2 = 6.06, p = 0.048) and metastases (χ2 =10.38, p = 0.001). Patients with high KAP 1 levels showed poor survival (p < 0.0001). Multivariate analysis showed that KAP1 high expression was an independent predictor for ovarian cancer patients (hazard ratio = 0.463; 95% confidence interval = 0.230-0.9318, p = 0.031). Functionally, depletion of KAP1 by siRNA inhibited ovarian cancer cell proliferation, cell migration. KAP1 expression correlated with aggressive clinical features in ovarian cancer. High KAP1 expression was a prognostic factor of ovarian cancer.

Show MeSH
Related in: MedlinePlus