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The regulation of nitric oxide synthase isoform expression in mouse and human fallopian tubes: potential insights for ectopic pregnancy.

Hu J, Ma S, Zou S, Li X, Cui P, Weijdegård B, Wu G, Shao R, Billig H, Feng Y - Int J Mol Sci (2014)

Bottom Line: Nitric oxide (NO) is highly unstable and has a half-life of seconds in buffer solutions.It is synthesized by NO-synthase (NOS), which has been found to exist in the following three isoforms: neuro nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS).Elevated iNOS activity might influence ovulation, cilia beats, contractility, and embryo transportation in such a manner as to increase the risk of ectopic pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Shanghai Medical College and Institute of Acupuncture Research (WHO Collaborating Center for Traditional Medicine), Institute of Brain Science, Fudan University, Shanghai 200032, China. 11211250005@fudan.edu.cn.

ABSTRACT
Nitric oxide (NO) is highly unstable and has a half-life of seconds in buffer solutions. It is synthesized by NO-synthase (NOS), which has been found to exist in the following three isoforms: neuro nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS). NOS activity is localized in the reproductive tracts of many species, although direct evidence for NOS isoforms in the Fallopian tubes of mice is still lacking. In the present study, we investigated the expression and regulation of NOS isoforms in the mouse and human Fallopian tubes during the estrous and menstrual cycles, respectively. We also measured isoform expression in humans with ectopic pregnancy and in mice treated with lipopolysaccharide (LPS). Our results confirmed the presence of different NOS isoforms in the mouse and human Fallopian tubes during different stages of the estrous and menstrual cycles and showed that iNOS expression increased in the Fallopian tubes of women with ectopic pregnancy and in LPS-treated mice. Elevated iNOS activity might influence ovulation, cilia beats, contractility, and embryo transportation in such a manner as to increase the risk of ectopic pregnancy. This study has provided morphological and molecular evidence that NOS isoforms are present and active in the human and mouse Fallopian tubes and suggests that iNOS might play an important role in both the reproductive cycle and infection-induced ectopic pregnancies.

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Quantitative RT-PCR analysis of iNOS (A) and eNOS (B) mRNA levels in adult mouse Fallopian tubes at different stages of the estrous cycle (n = 10/group). mRNA levels of each gene were relative to 18S rRNA levels in the same samples. Significance was tested by one-way ANOVA with Dunnett’s test, and no significant differences were found between any groups. Values are expressed as mean ± SEM.
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ijms-16-00049-f002: Quantitative RT-PCR analysis of iNOS (A) and eNOS (B) mRNA levels in adult mouse Fallopian tubes at different stages of the estrous cycle (n = 10/group). mRNA levels of each gene were relative to 18S rRNA levels in the same samples. Significance was tested by one-way ANOVA with Dunnett’s test, and no significant differences were found between any groups. Values are expressed as mean ± SEM.

Mentions: In this study, nNOS was not detected in the Fallopian tubes of C57/BL6 mice by qRT-PCR. To further confirm this, nine brains and Fallopian tubes from three strains of mice (C57/BL6, CBA, and ICR) were used to study the expression of nNOS by RT-PCR. The results showed that nNOS was expressed only in the mouse brain, and not in the mouse Fallopian tube (Figure 1). Both iNOS and eNOS mRNAs were abundant in the mouse Fallopian tube and were expressed in all stages of the estrous cycle, but there was no statistical difference between iNOS and eNOS mRNA levels (Figure 2).


The regulation of nitric oxide synthase isoform expression in mouse and human fallopian tubes: potential insights for ectopic pregnancy.

Hu J, Ma S, Zou S, Li X, Cui P, Weijdegård B, Wu G, Shao R, Billig H, Feng Y - Int J Mol Sci (2014)

Quantitative RT-PCR analysis of iNOS (A) and eNOS (B) mRNA levels in adult mouse Fallopian tubes at different stages of the estrous cycle (n = 10/group). mRNA levels of each gene were relative to 18S rRNA levels in the same samples. Significance was tested by one-way ANOVA with Dunnett’s test, and no significant differences were found between any groups. Values are expressed as mean ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307235&req=5

ijms-16-00049-f002: Quantitative RT-PCR analysis of iNOS (A) and eNOS (B) mRNA levels in adult mouse Fallopian tubes at different stages of the estrous cycle (n = 10/group). mRNA levels of each gene were relative to 18S rRNA levels in the same samples. Significance was tested by one-way ANOVA with Dunnett’s test, and no significant differences were found between any groups. Values are expressed as mean ± SEM.
Mentions: In this study, nNOS was not detected in the Fallopian tubes of C57/BL6 mice by qRT-PCR. To further confirm this, nine brains and Fallopian tubes from three strains of mice (C57/BL6, CBA, and ICR) were used to study the expression of nNOS by RT-PCR. The results showed that nNOS was expressed only in the mouse brain, and not in the mouse Fallopian tube (Figure 1). Both iNOS and eNOS mRNAs were abundant in the mouse Fallopian tube and were expressed in all stages of the estrous cycle, but there was no statistical difference between iNOS and eNOS mRNA levels (Figure 2).

Bottom Line: Nitric oxide (NO) is highly unstable and has a half-life of seconds in buffer solutions.It is synthesized by NO-synthase (NOS), which has been found to exist in the following three isoforms: neuro nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS).Elevated iNOS activity might influence ovulation, cilia beats, contractility, and embryo transportation in such a manner as to increase the risk of ectopic pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Shanghai Medical College and Institute of Acupuncture Research (WHO Collaborating Center for Traditional Medicine), Institute of Brain Science, Fudan University, Shanghai 200032, China. 11211250005@fudan.edu.cn.

ABSTRACT
Nitric oxide (NO) is highly unstable and has a half-life of seconds in buffer solutions. It is synthesized by NO-synthase (NOS), which has been found to exist in the following three isoforms: neuro nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS). NOS activity is localized in the reproductive tracts of many species, although direct evidence for NOS isoforms in the Fallopian tubes of mice is still lacking. In the present study, we investigated the expression and regulation of NOS isoforms in the mouse and human Fallopian tubes during the estrous and menstrual cycles, respectively. We also measured isoform expression in humans with ectopic pregnancy and in mice treated with lipopolysaccharide (LPS). Our results confirmed the presence of different NOS isoforms in the mouse and human Fallopian tubes during different stages of the estrous and menstrual cycles and showed that iNOS expression increased in the Fallopian tubes of women with ectopic pregnancy and in LPS-treated mice. Elevated iNOS activity might influence ovulation, cilia beats, contractility, and embryo transportation in such a manner as to increase the risk of ectopic pregnancy. This study has provided morphological and molecular evidence that NOS isoforms are present and active in the human and mouse Fallopian tubes and suggests that iNOS might play an important role in both the reproductive cycle and infection-induced ectopic pregnancies.

Show MeSH
Related in: MedlinePlus