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Genomic instability in complicated and uncomplicated Egyptian schistosomiasis haematobium patients.

Abd El-Aal AA, Bayoumy IR, Basyoni MM, Abd El-Aal AA, Emran AM, Abd El-Tawab MS, Badawi MA, Zalat RM, Diab TM - Mol Cytogenet (2015)

Bottom Line: In the acute uncomplicated group, nuclear-DNA of urinary epithelial cells was found diploid with mean nuclear-DNA content of 2.2 ± 0.16SD.The difference between nuclear DNA-contents in acute and chronic cases was significant (P = 0.0001).DNA morphometry was valuable in detection of gross genetic changes in urothelial tissues.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT

Background: Exploration of genetic changes during active Schistosoma infection is important for anticipation and prevention of chronic sequelae. This study aimed to explore the genomic instability in chromosomal and cellular kinetics in Egyptians suffering from uncomplicated active schistosomiasis haematobium infection in addition to chronic schistosomiasis haematobium cases complicated by bilharzial-associated bladder cancer (BAC).

Results: This study was conducted on 46 schistosomiasis haemotobium cases, 22 were active (Viable S. haematobium eggs in urine samples as detected by microscopy) and 24 were chronic complicated with bladder cancer. Three cytogenetic techniques were applied; the first was quantitative nuclear-morphocytometry by means of which the Feulgen-stained nuclei were analyzed for parameters including shape, size, integrated optical-density and nuclear area. The second was Fluorescent In-Situ Hybridization (FISH) for specific p53gene-locus of chromosome 17 and the third technique was karyotyping. Concerning chronic complicated cases, the mean ± SD of DNA-content in urinary bladder tissue sections was 3.18 ± 0.65. Five samples (20.83%) of bladder tissue sections of chronic complicated cases showed diploid nuclei, 6 urinary bladder tissue samples (25%) were tetraploid, while 13 bladder samples (54.16%) were aneuploid. Epithelial cells of urine samples demonstrated aneuploidy (mean ± SD = 3.74 ± 0.36).Nuclear contents showed high proliferative DNA index in all urinary epithelial cells. In the acute uncomplicated group, nuclear-DNA of urinary epithelial cells was found diploid with mean nuclear-DNA content of 2.2 ± 0.16SD. Half of these diploid smears had a high proliferation index. The difference between nuclear DNA-contents in acute and chronic cases was significant (P = 0.0001). FISH technique for specific p53gene-locus and karyotyping were done on urinary bladder tissue specimens and peripheral blood monocytes of 8 chronic cases respectively. Three samples (37.5%) with invasive BAC had a deletion of the p53 gene. Karyotyping showed three cases out of the 8 chronic schistosomiasis haematobium patients with chromosomal fragmentations.

Conclusions: DNA morphometry was valuable in detection of gross genetic changes in urothelial tissues. It is an important prognostic factor in established schistosomiasis haematobium induced bladder malignancy. It has the great advantage of being applicable on urine cells making it suitable for the prediction of a tendency towards genetic instability in active schistosomiasis haematobium patients.

No MeSH data available.


Related in: MedlinePlus

FISH images of formalin fixed paraffin embedded sections of BAC. Red signals (white arrow heads) are indicative of the p53 gene probe, while green signals (yellow block arrows) are indicative of centromeric probe of chromosome 17. The blue color is that of the DAPI counter stain. The presence of a single red signal per cell reflects the presence of a single copy of the p53 gene, i.e. p53 deletion (left), right photo represents normal cell.
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Fig3: FISH images of formalin fixed paraffin embedded sections of BAC. Red signals (white arrow heads) are indicative of the p53 gene probe, while green signals (yellow block arrows) are indicative of centromeric probe of chromosome 17. The blue color is that of the DAPI counter stain. The presence of a single red signal per cell reflects the presence of a single copy of the p53 gene, i.e. p53 deletion (left), right photo represents normal cell.

Mentions: One hundred cells were examined for each case. The cut offs were estimated by the clinical pathology laboratory by detection of the p53 gene locus deletion in 20 normal cases and calculating their mean and SD. Cases were considered positive if their score exceeded mean + SD of normal samples i.e. more than 6. Three samples (37.5%) were found to have a deletion of the p53 gene as evidenced by the presence of a single copy number of the gene (Figure 3).Figure 3


Genomic instability in complicated and uncomplicated Egyptian schistosomiasis haematobium patients.

Abd El-Aal AA, Bayoumy IR, Basyoni MM, Abd El-Aal AA, Emran AM, Abd El-Tawab MS, Badawi MA, Zalat RM, Diab TM - Mol Cytogenet (2015)

FISH images of formalin fixed paraffin embedded sections of BAC. Red signals (white arrow heads) are indicative of the p53 gene probe, while green signals (yellow block arrows) are indicative of centromeric probe of chromosome 17. The blue color is that of the DAPI counter stain. The presence of a single red signal per cell reflects the presence of a single copy of the p53 gene, i.e. p53 deletion (left), right photo represents normal cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4307227&req=5

Fig3: FISH images of formalin fixed paraffin embedded sections of BAC. Red signals (white arrow heads) are indicative of the p53 gene probe, while green signals (yellow block arrows) are indicative of centromeric probe of chromosome 17. The blue color is that of the DAPI counter stain. The presence of a single red signal per cell reflects the presence of a single copy of the p53 gene, i.e. p53 deletion (left), right photo represents normal cell.
Mentions: One hundred cells were examined for each case. The cut offs were estimated by the clinical pathology laboratory by detection of the p53 gene locus deletion in 20 normal cases and calculating their mean and SD. Cases were considered positive if their score exceeded mean + SD of normal samples i.e. more than 6. Three samples (37.5%) were found to have a deletion of the p53 gene as evidenced by the presence of a single copy number of the gene (Figure 3).Figure 3

Bottom Line: In the acute uncomplicated group, nuclear-DNA of urinary epithelial cells was found diploid with mean nuclear-DNA content of 2.2 ± 0.16SD.The difference between nuclear DNA-contents in acute and chronic cases was significant (P = 0.0001).DNA morphometry was valuable in detection of gross genetic changes in urothelial tissues.

View Article: PubMed Central - PubMed

Affiliation: Parasitology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

ABSTRACT

Background: Exploration of genetic changes during active Schistosoma infection is important for anticipation and prevention of chronic sequelae. This study aimed to explore the genomic instability in chromosomal and cellular kinetics in Egyptians suffering from uncomplicated active schistosomiasis haematobium infection in addition to chronic schistosomiasis haematobium cases complicated by bilharzial-associated bladder cancer (BAC).

Results: This study was conducted on 46 schistosomiasis haemotobium cases, 22 were active (Viable S. haematobium eggs in urine samples as detected by microscopy) and 24 were chronic complicated with bladder cancer. Three cytogenetic techniques were applied; the first was quantitative nuclear-morphocytometry by means of which the Feulgen-stained nuclei were analyzed for parameters including shape, size, integrated optical-density and nuclear area. The second was Fluorescent In-Situ Hybridization (FISH) for specific p53gene-locus of chromosome 17 and the third technique was karyotyping. Concerning chronic complicated cases, the mean ± SD of DNA-content in urinary bladder tissue sections was 3.18 ± 0.65. Five samples (20.83%) of bladder tissue sections of chronic complicated cases showed diploid nuclei, 6 urinary bladder tissue samples (25%) were tetraploid, while 13 bladder samples (54.16%) were aneuploid. Epithelial cells of urine samples demonstrated aneuploidy (mean ± SD = 3.74 ± 0.36).Nuclear contents showed high proliferative DNA index in all urinary epithelial cells. In the acute uncomplicated group, nuclear-DNA of urinary epithelial cells was found diploid with mean nuclear-DNA content of 2.2 ± 0.16SD. Half of these diploid smears had a high proliferation index. The difference between nuclear DNA-contents in acute and chronic cases was significant (P = 0.0001). FISH technique for specific p53gene-locus and karyotyping were done on urinary bladder tissue specimens and peripheral blood monocytes of 8 chronic cases respectively. Three samples (37.5%) with invasive BAC had a deletion of the p53 gene. Karyotyping showed three cases out of the 8 chronic schistosomiasis haematobium patients with chromosomal fragmentations.

Conclusions: DNA morphometry was valuable in detection of gross genetic changes in urothelial tissues. It is an important prognostic factor in established schistosomiasis haematobium induced bladder malignancy. It has the great advantage of being applicable on urine cells making it suitable for the prediction of a tendency towards genetic instability in active schistosomiasis haematobium patients.

No MeSH data available.


Related in: MedlinePlus