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Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress.

Zhang C, Zhang L, Zhang S, Zhu S, Wu P, Chen Y, Li M, Jiang H, Wu G - BMC Plant Biol. (2015)

Bottom Line: The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose.Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation.As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. ahzc2009@163.com.

ABSTRACT

Background: Physic nut (Jatropha curcas L.) is a small perennial tree or large shrub, which is well-adapted to semi-arid regions and is considered to have potential as a crop for biofuel production. It is now regarded as an excellent model for studying biofuel plants. However, our knowledge about the molecular responses of this species to drought stress is currently limited.

Results: In this study, genome-wide transcriptional profiles of roots and leaves of 8-week old physic nut seedlings were analyzed 1, 4 and 7 days after withholding irrigation. We observed a total of 1533 and 2900 differentially expressed genes (DEGs) in roots and leaves, respectively. Gene Ontology analysis showed that the biological processes enriched in droughted plants relative to unstressed plants were related to biosynthesis, transport, nucleobase-containing compounds, and cellular protein modification. The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose. Genes related to ABA signal transduction, and to trehalose and raffinose synthesis, were up-regulated in leaves. Endoplasmic reticulum (ER) stress response genes were significantly up-regulated in leaves under drought stress, while a number of genes related to wax biosynthesis were also up-regulated in leaves. Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation. As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

Conclusions: This study provides us with new insights to increase our understanding of the response mechanisms deployed by physic nut seedlings under drought stress. The genes and pathways identified in this study also provide much information of potential value for germplasm improvement and breeding for drought resistance.

No MeSH data available.


Related in: MedlinePlus

ABA biosynthesis, catabolism and signal transduction and the results of quantitative real-time PCR (qRT-PCR). A. ABA biosynthesis and catabolism; B. ABA signal transduction; C. qRT-PCR results for NCEDs, ABF and RD26. Relative expression level represents mean of n = 3 ± SD (Duncan test: *, P < 0.05). NCED, 9-cis-epoxycarotenoid dioxygenase; BGLU, beta glucosidase; CYP707A3, Cytochrome P450, family 707, subfamily A, polypeptide 3; ABA GT-ase, ABA Glycosyltransferase; AREB/ABF, abscisic acid responsive elements-binding factor; RD26, response to desiccation 26; HD-ZIP, homeodomain-leucine zipper protein; DREB, dehydration response element-binding protein.
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Fig3: ABA biosynthesis, catabolism and signal transduction and the results of quantitative real-time PCR (qRT-PCR). A. ABA biosynthesis and catabolism; B. ABA signal transduction; C. qRT-PCR results for NCEDs, ABF and RD26. Relative expression level represents mean of n = 3 ± SD (Duncan test: *, P < 0.05). NCED, 9-cis-epoxycarotenoid dioxygenase; BGLU, beta glucosidase; CYP707A3, Cytochrome P450, family 707, subfamily A, polypeptide 3; ABA GT-ase, ABA Glycosyltransferase; AREB/ABF, abscisic acid responsive elements-binding factor; RD26, response to desiccation 26; HD-ZIP, homeodomain-leucine zipper protein; DREB, dehydration response element-binding protein.

Mentions: To assess the accuracy of the digital expression data, genes involved in ABA biosynthesis and signal transduction (NCEDs, AREB/ABF and RD26) were tested by qRT-PCR (Figure 3). The results showed that two NCED genes (JC_C100001845 and JC_C100015061) were significantly up-regulated in roots under drought stress treatments, and two genes in an ABA dependent pathway, ABF (JC_C100011364) and RD26 (JC_C100019357), were significantly up-regulated in leaves (Figure 3C). The gene expression patterns obtained from qRT-PCR confirmed the results of Digital Gene Expression Profiling, indicating that the gene expression profiling approach used in this study was a reliable method for analyzing the response of physic nut to drought stress.Figure 3


Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress.

Zhang C, Zhang L, Zhang S, Zhu S, Wu P, Chen Y, Li M, Jiang H, Wu G - BMC Plant Biol. (2015)

ABA biosynthesis, catabolism and signal transduction and the results of quantitative real-time PCR (qRT-PCR). A. ABA biosynthesis and catabolism; B. ABA signal transduction; C. qRT-PCR results for NCEDs, ABF and RD26. Relative expression level represents mean of n = 3 ± SD (Duncan test: *, P < 0.05). NCED, 9-cis-epoxycarotenoid dioxygenase; BGLU, beta glucosidase; CYP707A3, Cytochrome P450, family 707, subfamily A, polypeptide 3; ABA GT-ase, ABA Glycosyltransferase; AREB/ABF, abscisic acid responsive elements-binding factor; RD26, response to desiccation 26; HD-ZIP, homeodomain-leucine zipper protein; DREB, dehydration response element-binding protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4307156&req=5

Fig3: ABA biosynthesis, catabolism and signal transduction and the results of quantitative real-time PCR (qRT-PCR). A. ABA biosynthesis and catabolism; B. ABA signal transduction; C. qRT-PCR results for NCEDs, ABF and RD26. Relative expression level represents mean of n = 3 ± SD (Duncan test: *, P < 0.05). NCED, 9-cis-epoxycarotenoid dioxygenase; BGLU, beta glucosidase; CYP707A3, Cytochrome P450, family 707, subfamily A, polypeptide 3; ABA GT-ase, ABA Glycosyltransferase; AREB/ABF, abscisic acid responsive elements-binding factor; RD26, response to desiccation 26; HD-ZIP, homeodomain-leucine zipper protein; DREB, dehydration response element-binding protein.
Mentions: To assess the accuracy of the digital expression data, genes involved in ABA biosynthesis and signal transduction (NCEDs, AREB/ABF and RD26) were tested by qRT-PCR (Figure 3). The results showed that two NCED genes (JC_C100001845 and JC_C100015061) were significantly up-regulated in roots under drought stress treatments, and two genes in an ABA dependent pathway, ABF (JC_C100011364) and RD26 (JC_C100019357), were significantly up-regulated in leaves (Figure 3C). The gene expression patterns obtained from qRT-PCR confirmed the results of Digital Gene Expression Profiling, indicating that the gene expression profiling approach used in this study was a reliable method for analyzing the response of physic nut to drought stress.Figure 3

Bottom Line: The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose.Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation.As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. ahzc2009@163.com.

ABSTRACT

Background: Physic nut (Jatropha curcas L.) is a small perennial tree or large shrub, which is well-adapted to semi-arid regions and is considered to have potential as a crop for biofuel production. It is now regarded as an excellent model for studying biofuel plants. However, our knowledge about the molecular responses of this species to drought stress is currently limited.

Results: In this study, genome-wide transcriptional profiles of roots and leaves of 8-week old physic nut seedlings were analyzed 1, 4 and 7 days after withholding irrigation. We observed a total of 1533 and 2900 differentially expressed genes (DEGs) in roots and leaves, respectively. Gene Ontology analysis showed that the biological processes enriched in droughted plants relative to unstressed plants were related to biosynthesis, transport, nucleobase-containing compounds, and cellular protein modification. The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose. Genes related to ABA signal transduction, and to trehalose and raffinose synthesis, were up-regulated in leaves. Endoplasmic reticulum (ER) stress response genes were significantly up-regulated in leaves under drought stress, while a number of genes related to wax biosynthesis were also up-regulated in leaves. Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation. As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

Conclusions: This study provides us with new insights to increase our understanding of the response mechanisms deployed by physic nut seedlings under drought stress. The genes and pathways identified in this study also provide much information of potential value for germplasm improvement and breeding for drought resistance.

No MeSH data available.


Related in: MedlinePlus