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Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress.

Zhang C, Zhang L, Zhang S, Zhu S, Wu P, Chen Y, Li M, Jiang H, Wu G - BMC Plant Biol. (2015)

Bottom Line: The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose.Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation.As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. ahzc2009@163.com.

ABSTRACT

Background: Physic nut (Jatropha curcas L.) is a small perennial tree or large shrub, which is well-adapted to semi-arid regions and is considered to have potential as a crop for biofuel production. It is now regarded as an excellent model for studying biofuel plants. However, our knowledge about the molecular responses of this species to drought stress is currently limited.

Results: In this study, genome-wide transcriptional profiles of roots and leaves of 8-week old physic nut seedlings were analyzed 1, 4 and 7 days after withholding irrigation. We observed a total of 1533 and 2900 differentially expressed genes (DEGs) in roots and leaves, respectively. Gene Ontology analysis showed that the biological processes enriched in droughted plants relative to unstressed plants were related to biosynthesis, transport, nucleobase-containing compounds, and cellular protein modification. The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose. Genes related to ABA signal transduction, and to trehalose and raffinose synthesis, were up-regulated in leaves. Endoplasmic reticulum (ER) stress response genes were significantly up-regulated in leaves under drought stress, while a number of genes related to wax biosynthesis were also up-regulated in leaves. Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation. As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

Conclusions: This study provides us with new insights to increase our understanding of the response mechanisms deployed by physic nut seedlings under drought stress. The genes and pathways identified in this study also provide much information of potential value for germplasm improvement and breeding for drought resistance.

No MeSH data available.


Gene Ontology (GO) analysis. BP, Biological Process; MF, Molecular Function; CC, Cellular Component.
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Fig2: Gene Ontology (GO) analysis. BP, Biological Process; MF, Molecular Function; CC, Cellular Component.

Mentions: All the DEGs were annotated using the results of blastp queries against the NCBI non-redundant protein sequences database and the Arabidopsis Information Resource Proteins database and the results were analyzed in AgBase (http://www.agbase.msstate.edu/). These GO annotations, including those for Cellular Component, Molecular Function and Biological Process, were collected and used to construct graphs (Figure 2). The three most highly enriched GO terms for Cellular Component were membrane, intracellular and cytoplasm, while transferase activity, hydrolase activity and nucleotide binding activity were the most enriched in the Molecular Function category (Figure 2). Biological Process annotations showed that biosynthesis, nucleobase-containing compound, transport, cellular protein modification process and response to stress were enriched in roots and leaves (Figure 2).Figure 2


Global analysis of gene expression profiles in physic nut (Jatropha curcas L.) seedlings exposed to drought stress.

Zhang C, Zhang L, Zhang S, Zhu S, Wu P, Chen Y, Li M, Jiang H, Wu G - BMC Plant Biol. (2015)

Gene Ontology (GO) analysis. BP, Biological Process; MF, Molecular Function; CC, Cellular Component.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4307156&req=5

Fig2: Gene Ontology (GO) analysis. BP, Biological Process; MF, Molecular Function; CC, Cellular Component.
Mentions: All the DEGs were annotated using the results of blastp queries against the NCBI non-redundant protein sequences database and the Arabidopsis Information Resource Proteins database and the results were analyzed in AgBase (http://www.agbase.msstate.edu/). These GO annotations, including those for Cellular Component, Molecular Function and Biological Process, were collected and used to construct graphs (Figure 2). The three most highly enriched GO terms for Cellular Component were membrane, intracellular and cytoplasm, while transferase activity, hydrolase activity and nucleotide binding activity were the most enriched in the Molecular Function category (Figure 2). Biological Process annotations showed that biosynthesis, nucleobase-containing compound, transport, cellular protein modification process and response to stress were enriched in roots and leaves (Figure 2).Figure 2

Bottom Line: The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose.Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation.As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. ahzc2009@163.com.

ABSTRACT

Background: Physic nut (Jatropha curcas L.) is a small perennial tree or large shrub, which is well-adapted to semi-arid regions and is considered to have potential as a crop for biofuel production. It is now regarded as an excellent model for studying biofuel plants. However, our knowledge about the molecular responses of this species to drought stress is currently limited.

Results: In this study, genome-wide transcriptional profiles of roots and leaves of 8-week old physic nut seedlings were analyzed 1, 4 and 7 days after withholding irrigation. We observed a total of 1533 and 2900 differentially expressed genes (DEGs) in roots and leaves, respectively. Gene Ontology analysis showed that the biological processes enriched in droughted plants relative to unstressed plants were related to biosynthesis, transport, nucleobase-containing compounds, and cellular protein modification. The genes found to be up-regulated in roots were related to abscisic acid (ABA) synthesis and ABA signal transduction, and to the synthesis of raffinose. Genes related to ABA signal transduction, and to trehalose and raffinose synthesis, were up-regulated in leaves. Endoplasmic reticulum (ER) stress response genes were significantly up-regulated in leaves under drought stress, while a number of genes related to wax biosynthesis were also up-regulated in leaves. Genes related to unsaturated fatty acid biosynthesis were down-regulated and polyunsaturated fatty acids were significantly reduced in leaves 7 days after withholding irrigation. As drought stress increased, genes related to ethylene synthesis, ethylene signal transduction and chlorophyll degradation were up-regulated, and the chlorophyll content of leaves was significantly reduced by 7 days after withholding irrigation.

Conclusions: This study provides us with new insights to increase our understanding of the response mechanisms deployed by physic nut seedlings under drought stress. The genes and pathways identified in this study also provide much information of potential value for germplasm improvement and breeding for drought resistance.

No MeSH data available.