Limits...
Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.

Heeren AM, van Iperen L, Klootwijk DB, de Melo Bernardo A, Roost MS, Gomes Fernandes MM, Louwe LA, Hilders CG, Helmerhorst FM, van der Westerlaken LA, Chuva de Sousa Lopes SM - BMC Dev. Biol. (2015)

Bottom Line: In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood.This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. marijneheeren@gmail.com.

ABSTRACT

Background: In society, there is a clear need to improve the success rate of techniques to restore fertility. Therefore a deeper knowledge of the dynamics of the complex molecular environment that regulates human gametogenesis and (early) folliculogenesis in vivo is necessary. Here, we have studied these processes focusing on the formation of the follicular basement membrane (BM) in vivo.

Results: The distribution of the main components of the extracellular matrix (ECM) collagen IV, laminin and fibronectin by week 10 of gestation (W10) in the ovarian cortex revealed the existence of ovarian cords and of a distinct mesenchymal compartment, resembling the organization in the male gonads. By W17, the first primordial follicles were assembled individually in that (cortical) mesenchymal compartment and were already encapsulated by a BM of collagen IV and laminin, but not fibronectin. In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.

Conclusions: The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood. This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

Show MeSH

Related in: MedlinePlus

Extracellular matrix components in the cortex of human adult ovarian tissue. (A-C) Collagen IV (A, A’), laminin (B, B’) and fibronectin (C, C’) expression (in green) in the BM underlying the OSE (white arrows) in the adult ovarian cortex. Scalebars are 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4307144&req=5

Fig7: Extracellular matrix components in the cortex of human adult ovarian tissue. (A-C) Collagen IV (A, A’), laminin (B, B’) and fibronectin (C, C’) expression (in green) in the BM underlying the OSE (white arrows) in the adult ovarian cortex. Scalebars are 50 μm.

Mentions: As in the fetal gonads, fibronectin expression was diffusely observed throughout the (cortical) mesenchyme and sporadically in the BM of secondary follicles (Figure 6D). Collagen IV, laminin and fibronectin expression was also detected diffusely throughout the (cortical) mesenchymal compartment of the ovarian cortex, but in particular in the tunica albuginea (Figure 7). Moreover, collagen IV and laminin, but not fibronectin, showed prominent expression in the BM under the OSE (Figure 7).Figure 7


Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.

Heeren AM, van Iperen L, Klootwijk DB, de Melo Bernardo A, Roost MS, Gomes Fernandes MM, Louwe LA, Hilders CG, Helmerhorst FM, van der Westerlaken LA, Chuva de Sousa Lopes SM - BMC Dev. Biol. (2015)

Extracellular matrix components in the cortex of human adult ovarian tissue. (A-C) Collagen IV (A, A’), laminin (B, B’) and fibronectin (C, C’) expression (in green) in the BM underlying the OSE (white arrows) in the adult ovarian cortex. Scalebars are 50 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4307144&req=5

Fig7: Extracellular matrix components in the cortex of human adult ovarian tissue. (A-C) Collagen IV (A, A’), laminin (B, B’) and fibronectin (C, C’) expression (in green) in the BM underlying the OSE (white arrows) in the adult ovarian cortex. Scalebars are 50 μm.
Mentions: As in the fetal gonads, fibronectin expression was diffusely observed throughout the (cortical) mesenchyme and sporadically in the BM of secondary follicles (Figure 6D). Collagen IV, laminin and fibronectin expression was also detected diffusely throughout the (cortical) mesenchymal compartment of the ovarian cortex, but in particular in the tunica albuginea (Figure 7). Moreover, collagen IV and laminin, but not fibronectin, showed prominent expression in the BM under the OSE (Figure 7).Figure 7

Bottom Line: In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood.This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. marijneheeren@gmail.com.

ABSTRACT

Background: In society, there is a clear need to improve the success rate of techniques to restore fertility. Therefore a deeper knowledge of the dynamics of the complex molecular environment that regulates human gametogenesis and (early) folliculogenesis in vivo is necessary. Here, we have studied these processes focusing on the formation of the follicular basement membrane (BM) in vivo.

Results: The distribution of the main components of the extracellular matrix (ECM) collagen IV, laminin and fibronectin by week 10 of gestation (W10) in the ovarian cortex revealed the existence of ovarian cords and of a distinct mesenchymal compartment, resembling the organization in the male gonads. By W17, the first primordial follicles were assembled individually in that (cortical) mesenchymal compartment and were already encapsulated by a BM of collagen IV and laminin, but not fibronectin. In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.

Conclusions: The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood. This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

Show MeSH
Related in: MedlinePlus