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Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.

Heeren AM, van Iperen L, Klootwijk DB, de Melo Bernardo A, Roost MS, Gomes Fernandes MM, Louwe LA, Hilders CG, Helmerhorst FM, van der Westerlaken LA, Chuva de Sousa Lopes SM - BMC Dev. Biol. (2015)

Bottom Line: In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood.This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. marijneheeren@gmail.com.

ABSTRACT

Background: In society, there is a clear need to improve the success rate of techniques to restore fertility. Therefore a deeper knowledge of the dynamics of the complex molecular environment that regulates human gametogenesis and (early) folliculogenesis in vivo is necessary. Here, we have studied these processes focusing on the formation of the follicular basement membrane (BM) in vivo.

Results: The distribution of the main components of the extracellular matrix (ECM) collagen IV, laminin and fibronectin by week 10 of gestation (W10) in the ovarian cortex revealed the existence of ovarian cords and of a distinct mesenchymal compartment, resembling the organization in the male gonads. By W17, the first primordial follicles were assembled individually in that (cortical) mesenchymal compartment and were already encapsulated by a BM of collagen IV and laminin, but not fibronectin. In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.

Conclusions: The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood. This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

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Related in: MedlinePlus

Collagen IV, laminin and fibronectin expression in human fetal female gonads during early development W7.3. (A,B) Collagen IV (green) expression identified the mesonepheric glomerulus, mesonephric and paramesonepheric ducts, and is present in the gonad. (C,D) laminin (green) (C), and fibronectin (green) (D) expression during early development of female gonads (W7.3). Germ cells (red) were identified by the early marker OCT4 (nuclear) and late marker VASA (cytoplasmic). The same secondary antibody was used to detect OCT4 and VASA, because the two proteins localize to different cellular compartments (nucleus and cytoplasm, respectively) and their expression can therefore be clearly distinguished. The white asterisks marked individual germ cells expressing collagen IV and laminin. Scalebars are 100 μm.
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Fig1: Collagen IV, laminin and fibronectin expression in human fetal female gonads during early development W7.3. (A,B) Collagen IV (green) expression identified the mesonepheric glomerulus, mesonephric and paramesonepheric ducts, and is present in the gonad. (C,D) laminin (green) (C), and fibronectin (green) (D) expression during early development of female gonads (W7.3). Germ cells (red) were identified by the early marker OCT4 (nuclear) and late marker VASA (cytoplasmic). The same secondary antibody was used to detect OCT4 and VASA, because the two proteins localize to different cellular compartments (nucleus and cytoplasm, respectively) and their expression can therefore be clearly distinguished. The white asterisks marked individual germ cells expressing collagen IV and laminin. Scalebars are 100 μm.

Mentions: At W6-7, we observed a low number of germ cells colonizing the human female gonads (Figure 1). Many structures in the mesonephros, including the mesonephric glomeruli, tubuli and duct, were clearly delineated by a BM rich in collagen IV, however the developing gonad contained only few collagen IV fibrils without an apparent organization (Figure 1A and 1B). Laminin and fibronectin also seemed ubiquitously present in the female gonad (Figure 1C and 1D).Figure 1


Development of the follicular basement membrane during human gametogenesis and early folliculogenesis.

Heeren AM, van Iperen L, Klootwijk DB, de Melo Bernardo A, Roost MS, Gomes Fernandes MM, Louwe LA, Hilders CG, Helmerhorst FM, van der Westerlaken LA, Chuva de Sousa Lopes SM - BMC Dev. Biol. (2015)

Collagen IV, laminin and fibronectin expression in human fetal female gonads during early development W7.3. (A,B) Collagen IV (green) expression identified the mesonepheric glomerulus, mesonephric and paramesonepheric ducts, and is present in the gonad. (C,D) laminin (green) (C), and fibronectin (green) (D) expression during early development of female gonads (W7.3). Germ cells (red) were identified by the early marker OCT4 (nuclear) and late marker VASA (cytoplasmic). The same secondary antibody was used to detect OCT4 and VASA, because the two proteins localize to different cellular compartments (nucleus and cytoplasm, respectively) and their expression can therefore be clearly distinguished. The white asterisks marked individual germ cells expressing collagen IV and laminin. Scalebars are 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4307144&req=5

Fig1: Collagen IV, laminin and fibronectin expression in human fetal female gonads during early development W7.3. (A,B) Collagen IV (green) expression identified the mesonepheric glomerulus, mesonephric and paramesonepheric ducts, and is present in the gonad. (C,D) laminin (green) (C), and fibronectin (green) (D) expression during early development of female gonads (W7.3). Germ cells (red) were identified by the early marker OCT4 (nuclear) and late marker VASA (cytoplasmic). The same secondary antibody was used to detect OCT4 and VASA, because the two proteins localize to different cellular compartments (nucleus and cytoplasm, respectively) and their expression can therefore be clearly distinguished. The white asterisks marked individual germ cells expressing collagen IV and laminin. Scalebars are 100 μm.
Mentions: At W6-7, we observed a low number of germ cells colonizing the human female gonads (Figure 1). Many structures in the mesonephros, including the mesonephric glomeruli, tubuli and duct, were clearly delineated by a BM rich in collagen IV, however the developing gonad contained only few collagen IV fibrils without an apparent organization (Figure 1A and 1B). Laminin and fibronectin also seemed ubiquitously present in the female gonad (Figure 1C and 1D).Figure 1

Bottom Line: In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood.This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333, Leiden, ZC, The Netherlands. marijneheeren@gmail.com.

ABSTRACT

Background: In society, there is a clear need to improve the success rate of techniques to restore fertility. Therefore a deeper knowledge of the dynamics of the complex molecular environment that regulates human gametogenesis and (early) folliculogenesis in vivo is necessary. Here, we have studied these processes focusing on the formation of the follicular basement membrane (BM) in vivo.

Results: The distribution of the main components of the extracellular matrix (ECM) collagen IV, laminin and fibronectin by week 10 of gestation (W10) in the ovarian cortex revealed the existence of ovarian cords and of a distinct mesenchymal compartment, resembling the organization in the male gonads. By W17, the first primordial follicles were assembled individually in that (cortical) mesenchymal compartment and were already encapsulated by a BM of collagen IV and laminin, but not fibronectin. In adults, in the primary and secondary follicles, collagen IV, laminin and to a lesser extent fibronectin were prominent in the follicular BM.

Conclusions: The ECM-molecular niche compartimentalizes the female gonads from the time of germ cell colonization until adulthood. This knowledge may contribute to improve methods to recreate the environment needed for successful folliculogenesis in vitro and that would benefit a large number of infertility patients.

Show MeSH
Related in: MedlinePlus