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Efficient lentiviral transduction of adipose tissue-derived mouse mesenchymal stem cells and assessment of their penetration in female mice cervical tumor model.

Kenarkoohi A, Soleimani M, Bamdad T, Soleimanjahi H, Estiri H, Razavi-Nikoo MH - Iran J Cancer Prev (2014)

Bottom Line: In our study, lentiviral vector transductions of MSCs performed.Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: Although the incidence of cervical cancer has reduced during last years, but it causes mortality among women. Many efforts have performed to develop new drugs and strategy for treatment of cervical cancer. Adipose Tissue-Derived mouse Mesenchymal Stem Cells (MSCs) has many advantages which make them a suitable choice as a cell therapeutic agent in cancer treatment. In this study, we aimed to develop an improved protocol for Mouse MSCs transduction as well as assess the homing capacity and incorporation of MSCs in cervical cancer model.

Methods: MScs were isolated from the mouse adipose tissue and characterized by differentiation and flow cytometry. In our study, lentiviral vector transductions of MSCs performed. Their penetrations were detected in tissue sections after injection of transduced MSCs to female C57BL/6 mice as a cervical cancer model.

Results: Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.

Conclusion: The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

No MeSH data available.


Related in: MedlinePlus

A: Transductions were carried out in the presence of 8 μg of Polybrene (Sigma) per ml. After incubation at 37°C for 48 h, transduced MSCs seen by Fluorescence microscopy. High expression of GFP in MSCs shows the high rate of transduction. B: shows transduced MScs by light microscopy after incubation at 37°C for 48 h.
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f4-IJCP-07-225: A: Transductions were carried out in the presence of 8 μg of Polybrene (Sigma) per ml. After incubation at 37°C for 48 h, transduced MSCs seen by Fluorescence microscopy. High expression of GFP in MSCs shows the high rate of transduction. B: shows transduced MScs by light microscopy after incubation at 37°C for 48 h.

Mentions: MSCs were plated 24 h before transduction at a density of 50,000 cells per well of 6-well plates. Transduction was performed three times with 8 h intervals. In first time, cells were infected with lentiviral vector as well as 8 μg/mL polybrene and the plates were centrifuged in 2000 rpm for 1 h (Figure 4). In other twice, same MSCs were infected only with lentiviral vector.


Efficient lentiviral transduction of adipose tissue-derived mouse mesenchymal stem cells and assessment of their penetration in female mice cervical tumor model.

Kenarkoohi A, Soleimani M, Bamdad T, Soleimanjahi H, Estiri H, Razavi-Nikoo MH - Iran J Cancer Prev (2014)

A: Transductions were carried out in the presence of 8 μg of Polybrene (Sigma) per ml. After incubation at 37°C for 48 h, transduced MSCs seen by Fluorescence microscopy. High expression of GFP in MSCs shows the high rate of transduction. B: shows transduced MScs by light microscopy after incubation at 37°C for 48 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307105&req=5

f4-IJCP-07-225: A: Transductions were carried out in the presence of 8 μg of Polybrene (Sigma) per ml. After incubation at 37°C for 48 h, transduced MSCs seen by Fluorescence microscopy. High expression of GFP in MSCs shows the high rate of transduction. B: shows transduced MScs by light microscopy after incubation at 37°C for 48 h.
Mentions: MSCs were plated 24 h before transduction at a density of 50,000 cells per well of 6-well plates. Transduction was performed three times with 8 h intervals. In first time, cells were infected with lentiviral vector as well as 8 μg/mL polybrene and the plates were centrifuged in 2000 rpm for 1 h (Figure 4). In other twice, same MSCs were infected only with lentiviral vector.

Bottom Line: In our study, lentiviral vector transductions of MSCs performed.Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: Although the incidence of cervical cancer has reduced during last years, but it causes mortality among women. Many efforts have performed to develop new drugs and strategy for treatment of cervical cancer. Adipose Tissue-Derived mouse Mesenchymal Stem Cells (MSCs) has many advantages which make them a suitable choice as a cell therapeutic agent in cancer treatment. In this study, we aimed to develop an improved protocol for Mouse MSCs transduction as well as assess the homing capacity and incorporation of MSCs in cervical cancer model.

Methods: MScs were isolated from the mouse adipose tissue and characterized by differentiation and flow cytometry. In our study, lentiviral vector transductions of MSCs performed. Their penetrations were detected in tissue sections after injection of transduced MSCs to female C57BL/6 mice as a cervical cancer model.

Results: Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.

Conclusion: The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

No MeSH data available.


Related in: MedlinePlus