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Efficient lentiviral transduction of adipose tissue-derived mouse mesenchymal stem cells and assessment of their penetration in female mice cervical tumor model.

Kenarkoohi A, Soleimani M, Bamdad T, Soleimanjahi H, Estiri H, Razavi-Nikoo MH - Iran J Cancer Prev (2014)

Bottom Line: In our study, lentiviral vector transductions of MSCs performed.Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: Although the incidence of cervical cancer has reduced during last years, but it causes mortality among women. Many efforts have performed to develop new drugs and strategy for treatment of cervical cancer. Adipose Tissue-Derived mouse Mesenchymal Stem Cells (MSCs) has many advantages which make them a suitable choice as a cell therapeutic agent in cancer treatment. In this study, we aimed to develop an improved protocol for Mouse MSCs transduction as well as assess the homing capacity and incorporation of MSCs in cervical cancer model.

Methods: MScs were isolated from the mouse adipose tissue and characterized by differentiation and flow cytometry. In our study, lentiviral vector transductions of MSCs performed. Their penetrations were detected in tissue sections after injection of transduced MSCs to female C57BL/6 mice as a cervical cancer model.

Results: Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.

Conclusion: The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

No MeSH data available.


Related in: MedlinePlus

Isolation of MSCs from mouse adipose tissue and characterized by differentiationA: Adipo MSCs were cultured in osteogenic differentiation medium and after 21 days of culture stained with Alizarin red. Alizarin red stained the calcium deposits. Formation calcium deposits indicate that osteogenic differentiation process performed.B: Figure B shows MSCs were differentiated to adipocyte. Lipid Vesicle seen in cells with Oil Red staining and indicated successful adipogenic differentiation. C: Figure c shows the MSCs at 3rd passage.
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f2-IJCP-07-225: Isolation of MSCs from mouse adipose tissue and characterized by differentiationA: Adipo MSCs were cultured in osteogenic differentiation medium and after 21 days of culture stained with Alizarin red. Alizarin red stained the calcium deposits. Formation calcium deposits indicate that osteogenic differentiation process performed.B: Figure B shows MSCs were differentiated to adipocyte. Lipid Vesicle seen in cells with Oil Red staining and indicated successful adipogenic differentiation. C: Figure c shows the MSCs at 3rd passage.

Mentions: 21 days after culture the MSCs with osteogenic-inducing media at three passages, osteogenic differentiation was observed with alizarin red staining (Figure 2A).


Efficient lentiviral transduction of adipose tissue-derived mouse mesenchymal stem cells and assessment of their penetration in female mice cervical tumor model.

Kenarkoohi A, Soleimani M, Bamdad T, Soleimanjahi H, Estiri H, Razavi-Nikoo MH - Iran J Cancer Prev (2014)

Isolation of MSCs from mouse adipose tissue and characterized by differentiationA: Adipo MSCs were cultured in osteogenic differentiation medium and after 21 days of culture stained with Alizarin red. Alizarin red stained the calcium deposits. Formation calcium deposits indicate that osteogenic differentiation process performed.B: Figure B shows MSCs were differentiated to adipocyte. Lipid Vesicle seen in cells with Oil Red staining and indicated successful adipogenic differentiation. C: Figure c shows the MSCs at 3rd passage.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307105&req=5

f2-IJCP-07-225: Isolation of MSCs from mouse adipose tissue and characterized by differentiationA: Adipo MSCs were cultured in osteogenic differentiation medium and after 21 days of culture stained with Alizarin red. Alizarin red stained the calcium deposits. Formation calcium deposits indicate that osteogenic differentiation process performed.B: Figure B shows MSCs were differentiated to adipocyte. Lipid Vesicle seen in cells with Oil Red staining and indicated successful adipogenic differentiation. C: Figure c shows the MSCs at 3rd passage.
Mentions: 21 days after culture the MSCs with osteogenic-inducing media at three passages, osteogenic differentiation was observed with alizarin red staining (Figure 2A).

Bottom Line: In our study, lentiviral vector transductions of MSCs performed.Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

ABSTRACT

Background: Although the incidence of cervical cancer has reduced during last years, but it causes mortality among women. Many efforts have performed to develop new drugs and strategy for treatment of cervical cancer. Adipose Tissue-Derived mouse Mesenchymal Stem Cells (MSCs) has many advantages which make them a suitable choice as a cell therapeutic agent in cancer treatment. In this study, we aimed to develop an improved protocol for Mouse MSCs transduction as well as assess the homing capacity and incorporation of MSCs in cervical cancer model.

Methods: MScs were isolated from the mouse adipose tissue and characterized by differentiation and flow cytometry. In our study, lentiviral vector transductions of MSCs performed. Their penetrations were detected in tissue sections after injection of transduced MSCs to female C57BL/6 mice as a cervical cancer model.

Results: Results showed that MSCs were efficiently transduced with lentiviral vector resulting in efficient tumor penetration.

Conclusion: The results provide evidence that MSCs were able to penetrate into the tumor mass of cervical tumor model and are good vehicles for gene transfer to cervical cancer.

No MeSH data available.


Related in: MedlinePlus