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The effect of interleukin 36 gene therapy in the regression of tumor.

Solahaye-Kahnamouii S, Farhadi F, Rahkare-Farshi M, Pakdel F, Kashefimehr A, Pouralibaba F, Shirani G, Bayat M, Karimi A - Iran J Cancer Prev (2014)

Bottom Line: In this study we had 2 groups which are treated with IL-36 and Untreated with IL-36 as a blank.The results of western blotting and real-time PCR showed the IL36 expression increased in the group treated with IL36 (with relative expression of 1.9).Immunohistochemistry staining indicated that the Ki-67expression has been reduced in the group interfered with IL36.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral Maxillofacial Surgery, Tehran University of Medical Science, Tehran, Iran.

ABSTRACT

Background: Cancer immunotherapy attempts to stimulate the immune system to reject and destroy tumors and is one of the cancer treatment strategies. Recently, interluekin36 (IL36) has been used as immunotherapeutic agents in cancer gene therapy. Present study investigated that the IL36 gene therapy effects on the regression of tumor masses in mouse model. Aim of this study is determination of the gene therapy effects by IL36 in the regression of tumor masses in mouse model.

Methods: To study the therapeutic efficacy of this cytokine, WEHI-164 tumor cells were transected with mIL36 plasmids. ELISA test was used to check cytokine production by transected cells. To establish fibro sarcoma mouse model, Tumoral transfected cells were injected subcutaneously to inoculate tumor in BALB/C mice. Tumor volumes were measured by caliper. Mice were sacrificed and tumors were extracted. The expression of IL36 and IFN-γ was studied with Real-time PCR and immunoblotting. The expression of Ki-67 (a tumor proliferation marker) in tumor masses was studied by immunohistochemistry staining. In this study we had 2 groups which are treated with IL-36 and Untreated with IL-36 as a blank.

Results: The group treated with IL36 indicated decrease of tumor mass volume (p<0.001). The results of western blotting and real-time PCR showed the IL36 expression increased in the group treated with IL36 (with relative expression of 1.9).

Conclusion: Immunohistochemistry staining indicated that the Ki-67expression has been reduced in the group interfered with IL36. IL36 gene therapy has therapeutic effects on the regression of tumor masses in fibro sarcoma mouse model.

No MeSH data available.


Related in: MedlinePlus

Tumor mass regression in group treated with IL36. The results showed that the tumor mass volume was reduced in group treated with IL36 (Sig: 0.00).
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f2-IJCP-07-197: Tumor mass regression in group treated with IL36. The results showed that the tumor mass volume was reduced in group treated with IL36 (Sig: 0.00).

Mentions: To test the antitumor response induced by IL36 secreted in site, BALB/C mice (n=7) were injected subcutaneously with fibro sarcoma/IL36 cells (1×106), and monitored for tumor growth 3 times a week. After 21 days of tumor inoculation the volume of the tumor masses was less than control group. So gene therapy with IL36 has effect in the regression of tumor masses (Figure 2).


The effect of interleukin 36 gene therapy in the regression of tumor.

Solahaye-Kahnamouii S, Farhadi F, Rahkare-Farshi M, Pakdel F, Kashefimehr A, Pouralibaba F, Shirani G, Bayat M, Karimi A - Iran J Cancer Prev (2014)

Tumor mass regression in group treated with IL36. The results showed that the tumor mass volume was reduced in group treated with IL36 (Sig: 0.00).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4307102&req=5

f2-IJCP-07-197: Tumor mass regression in group treated with IL36. The results showed that the tumor mass volume was reduced in group treated with IL36 (Sig: 0.00).
Mentions: To test the antitumor response induced by IL36 secreted in site, BALB/C mice (n=7) were injected subcutaneously with fibro sarcoma/IL36 cells (1×106), and monitored for tumor growth 3 times a week. After 21 days of tumor inoculation the volume of the tumor masses was less than control group. So gene therapy with IL36 has effect in the regression of tumor masses (Figure 2).

Bottom Line: In this study we had 2 groups which are treated with IL-36 and Untreated with IL-36 as a blank.The results of western blotting and real-time PCR showed the IL36 expression increased in the group treated with IL36 (with relative expression of 1.9).Immunohistochemistry staining indicated that the Ki-67expression has been reduced in the group interfered with IL36.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Oral Maxillofacial Surgery, Tehran University of Medical Science, Tehran, Iran.

ABSTRACT

Background: Cancer immunotherapy attempts to stimulate the immune system to reject and destroy tumors and is one of the cancer treatment strategies. Recently, interluekin36 (IL36) has been used as immunotherapeutic agents in cancer gene therapy. Present study investigated that the IL36 gene therapy effects on the regression of tumor masses in mouse model. Aim of this study is determination of the gene therapy effects by IL36 in the regression of tumor masses in mouse model.

Methods: To study the therapeutic efficacy of this cytokine, WEHI-164 tumor cells were transected with mIL36 plasmids. ELISA test was used to check cytokine production by transected cells. To establish fibro sarcoma mouse model, Tumoral transfected cells were injected subcutaneously to inoculate tumor in BALB/C mice. Tumor volumes were measured by caliper. Mice were sacrificed and tumors were extracted. The expression of IL36 and IFN-γ was studied with Real-time PCR and immunoblotting. The expression of Ki-67 (a tumor proliferation marker) in tumor masses was studied by immunohistochemistry staining. In this study we had 2 groups which are treated with IL-36 and Untreated with IL-36 as a blank.

Results: The group treated with IL36 indicated decrease of tumor mass volume (p<0.001). The results of western blotting and real-time PCR showed the IL36 expression increased in the group treated with IL36 (with relative expression of 1.9).

Conclusion: Immunohistochemistry staining indicated that the Ki-67expression has been reduced in the group interfered with IL36. IL36 gene therapy has therapeutic effects on the regression of tumor masses in fibro sarcoma mouse model.

No MeSH data available.


Related in: MedlinePlus