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Royal Jelly alleviates sperm toxicity and improves in vitro fertilization outcome in Stanozolol-treated mice.

Shalizar Jalali A, Najafi G, Hosseinchi M, Sedighnia A - Iran J Reprod Med (2015)

Bottom Line: ST treatment caused a significant (p<0.05) decrease in sperm count and motility and fertilization rate along with poor blastocyst formation and increased sperm DNA damage.The above-mentioned parameters were restored to near normal level by RJ co-administration.However, clinical studies are warranted to investigate such an effect in human subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

ABSTRACT

Background: Stanozolol (ST) is a synthetic anabolic-androgenic steroid often abused by athletes. An increasing body of evidence points towards the role of ST misuses in the pathogenesis of a wide range of adverse effects including reprotoxicity.

Objective: The aim of this study was to analyze the possible reproprotective effect of royal jelly (RJ) as an efficient antioxidant in ST-treated mice.

Materials and methods: Adult male mice were divided into four groups (n=5). Two groups of mice received ST (4.6 mg/kg/day) via gavage for 35 days. RJ was given orally to one of these groups at the dose level of 100 mg/kg body weight per day synchronously. Untreated control group and RJ-only treated group were also included. Epididymal sperm characteristics and in vitro fertilizing capacity were evaluated after 35 days.

Results: ST treatment caused a significant (p<0.05) decrease in sperm count and motility and fertilization rate along with poor blastocyst formation and increased sperm DNA damage. Moreover, the incidence of apoptosis and abnormality in spermatozoa was significantly (p<0.05) higher in ST-exposed mice than those of control. The above-mentioned parameters were restored to near normal level by RJ co-administration.

Conclusion: Data from the current study suggest that RJ has a potential repro-protective action against ST-induced reproductive toxicity in mice. However, clinical studies are warranted to investigate such an effect in human subjects.

No MeSH data available.


Related in: MedlinePlus

Effect of stanozolol and royal jelly treatment on sperm apoptosis in mature male mice. Histogram represents apoptotic index expressed as apoptotic sperm cells per 100 spermatozoa.
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Figure 1: Effect of stanozolol and royal jelly treatment on sperm apoptosis in mature male mice. Histogram represents apoptotic index expressed as apoptotic sperm cells per 100 spermatozoa.

Mentions: Figure 1 represents data concerning sperm cell apoptosis in all experimental groups. As expected, AI was significantly higher in the ST group versus the control and RJ alone groups, whilst RJ co-administration substantially reduced AI as compared to the ST group non-treated with RJ.


Royal Jelly alleviates sperm toxicity and improves in vitro fertilization outcome in Stanozolol-treated mice.

Shalizar Jalali A, Najafi G, Hosseinchi M, Sedighnia A - Iran J Reprod Med (2015)

Effect of stanozolol and royal jelly treatment on sperm apoptosis in mature male mice. Histogram represents apoptotic index expressed as apoptotic sperm cells per 100 spermatozoa.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306980&req=5

Figure 1: Effect of stanozolol and royal jelly treatment on sperm apoptosis in mature male mice. Histogram represents apoptotic index expressed as apoptotic sperm cells per 100 spermatozoa.
Mentions: Figure 1 represents data concerning sperm cell apoptosis in all experimental groups. As expected, AI was significantly higher in the ST group versus the control and RJ alone groups, whilst RJ co-administration substantially reduced AI as compared to the ST group non-treated with RJ.

Bottom Line: ST treatment caused a significant (p<0.05) decrease in sperm count and motility and fertilization rate along with poor blastocyst formation and increased sperm DNA damage.The above-mentioned parameters were restored to near normal level by RJ co-administration.However, clinical studies are warranted to investigate such an effect in human subjects.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

ABSTRACT

Background: Stanozolol (ST) is a synthetic anabolic-androgenic steroid often abused by athletes. An increasing body of evidence points towards the role of ST misuses in the pathogenesis of a wide range of adverse effects including reprotoxicity.

Objective: The aim of this study was to analyze the possible reproprotective effect of royal jelly (RJ) as an efficient antioxidant in ST-treated mice.

Materials and methods: Adult male mice were divided into four groups (n=5). Two groups of mice received ST (4.6 mg/kg/day) via gavage for 35 days. RJ was given orally to one of these groups at the dose level of 100 mg/kg body weight per day synchronously. Untreated control group and RJ-only treated group were also included. Epididymal sperm characteristics and in vitro fertilizing capacity were evaluated after 35 days.

Results: ST treatment caused a significant (p<0.05) decrease in sperm count and motility and fertilization rate along with poor blastocyst formation and increased sperm DNA damage. Moreover, the incidence of apoptosis and abnormality in spermatozoa was significantly (p<0.05) higher in ST-exposed mice than those of control. The above-mentioned parameters were restored to near normal level by RJ co-administration.

Conclusion: Data from the current study suggest that RJ has a potential repro-protective action against ST-induced reproductive toxicity in mice. However, clinical studies are warranted to investigate such an effect in human subjects.

No MeSH data available.


Related in: MedlinePlus