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Squid pen chitin chitooligomers as food colorants absorbers.

Liang TW, Huang CT, Dzung NA, Wang SL - Mar Drugs (2015)

Bottom Line: In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4).Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption.Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. QQ1987pp@hotmail.com.

ABSTRACT
One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

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fourier transform infrared spectra (FT-IR) spectra of fermented SPP by B. cereus TKU033 before (a); and after the adsorption of R40 (b); and Y4 (c).
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marinedrugs-13-00681-f005: fourier transform infrared spectra (FT-IR) spectra of fermented SPP by B. cereus TKU033 before (a); and after the adsorption of R40 (b); and Y4 (c).

Mentions: The fourier transform infrared spectra (FTIR) of the fermented SPP before and after the adsorption of a food colorant (R40 and Y4) are shown in Figure 5. There was no significant change in the spectra at wavenumbers 3289 cm−1 (-OH stretching vibration), 3082 cm−1 (C-H stretching), 2879 cm−1 (-CH2 stretching) and 1647 cm−1 (-NH stretching vibration) after the adsorption of the dyes. This implies that the adsorption processes represent a physical adsorption and may not involve a chemical interaction. These results provide a summary of the available information on SPP and its potential as a low-cost sorbent.


Squid pen chitin chitooligomers as food colorants absorbers.

Liang TW, Huang CT, Dzung NA, Wang SL - Mar Drugs (2015)

fourier transform infrared spectra (FT-IR) spectra of fermented SPP by B. cereus TKU033 before (a); and after the adsorption of R40 (b); and Y4 (c).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306958&req=5

marinedrugs-13-00681-f005: fourier transform infrared spectra (FT-IR) spectra of fermented SPP by B. cereus TKU033 before (a); and after the adsorption of R40 (b); and Y4 (c).
Mentions: The fourier transform infrared spectra (FTIR) of the fermented SPP before and after the adsorption of a food colorant (R40 and Y4) are shown in Figure 5. There was no significant change in the spectra at wavenumbers 3289 cm−1 (-OH stretching vibration), 3082 cm−1 (C-H stretching), 2879 cm−1 (-CH2 stretching) and 1647 cm−1 (-NH stretching vibration) after the adsorption of the dyes. This implies that the adsorption processes represent a physical adsorption and may not involve a chemical interaction. These results provide a summary of the available information on SPP and its potential as a low-cost sorbent.

Bottom Line: In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4).Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption.Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. QQ1987pp@hotmail.com.

ABSTRACT
One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

Show MeSH