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Squid pen chitin chitooligomers as food colorants absorbers.

Liang TW, Huang CT, Dzung NA, Wang SL - Mar Drugs (2015)

Bottom Line: In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4).Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption.Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. QQ1987pp@hotmail.com.

ABSTRACT
One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

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Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the chitosanase produced by B. cereus TKU033. Lanes: M, molecular markers (170, 130, 95, 72, 55, 43, 34, 26, 17, and 10 kDa); (1) crude enzyme; (2) adsorbed chitosanase fractions after DEAE-Sepharose CL-6B chromatography; (3) adsorbed chitosanase fractions after Macro-prep DEAE chromatography.
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marinedrugs-13-00681-f002: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the chitosanase produced by B. cereus TKU033. Lanes: M, molecular markers (170, 130, 95, 72, 55, 43, 34, 26, 17, and 10 kDa); (1) crude enzyme; (2) adsorbed chitosanase fractions after DEAE-Sepharose CL-6B chromatography; (3) adsorbed chitosanase fractions after Macro-prep DEAE chromatography.

Mentions: Extracellular chitosanase was purified from the culture supernatant of B. cereus TKU033 using a series of purification procedures. The TKU033 chitosanase was eluted in the DEAE-Sepharose CL-6B chromatography step with a linear gradient of 0–1 M NaCl in the same buffer. The eluted peak fractions were pooled for further purification. After the Macro-prep DEAE chromatography step (data not shown), approximately 5.8 mg of TKU033 chitosanase was obtained (Table 1). A summary of the purification process is presented in Table 1. The purification steps were combined to give approximately an overall 10.4-fold purification of the TKU033 chitosanase. The overall TKU033 chitosanase activity yield was 2% with a specific activity of 0.052 U/mg. The molecular mass of the TKU033 chitosanase was approximately 43 kDa as confirmed by the SDS-PAGE (Figure 2), which corresponded to the gel-filtration chromatography. The molecular mass of the TKU033 chitosanase (43 kDa) was similar to most chitosanases, which have a medium apparent molecular mass within the range of 20–75 kDa [7,20].


Squid pen chitin chitooligomers as food colorants absorbers.

Liang TW, Huang CT, Dzung NA, Wang SL - Mar Drugs (2015)

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the chitosanase produced by B. cereus TKU033. Lanes: M, molecular markers (170, 130, 95, 72, 55, 43, 34, 26, 17, and 10 kDa); (1) crude enzyme; (2) adsorbed chitosanase fractions after DEAE-Sepharose CL-6B chromatography; (3) adsorbed chitosanase fractions after Macro-prep DEAE chromatography.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306958&req=5

marinedrugs-13-00681-f002: Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the chitosanase produced by B. cereus TKU033. Lanes: M, molecular markers (170, 130, 95, 72, 55, 43, 34, 26, 17, and 10 kDa); (1) crude enzyme; (2) adsorbed chitosanase fractions after DEAE-Sepharose CL-6B chromatography; (3) adsorbed chitosanase fractions after Macro-prep DEAE chromatography.
Mentions: Extracellular chitosanase was purified from the culture supernatant of B. cereus TKU033 using a series of purification procedures. The TKU033 chitosanase was eluted in the DEAE-Sepharose CL-6B chromatography step with a linear gradient of 0–1 M NaCl in the same buffer. The eluted peak fractions were pooled for further purification. After the Macro-prep DEAE chromatography step (data not shown), approximately 5.8 mg of TKU033 chitosanase was obtained (Table 1). A summary of the purification process is presented in Table 1. The purification steps were combined to give approximately an overall 10.4-fold purification of the TKU033 chitosanase. The overall TKU033 chitosanase activity yield was 2% with a specific activity of 0.052 U/mg. The molecular mass of the TKU033 chitosanase was approximately 43 kDa as confirmed by the SDS-PAGE (Figure 2), which corresponded to the gel-filtration chromatography. The molecular mass of the TKU033 chitosanase (43 kDa) was similar to most chitosanases, which have a medium apparent molecular mass within the range of 20–75 kDa [7,20].

Bottom Line: In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4).Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption.Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. QQ1987pp@hotmail.com.

ABSTRACT
One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

Show MeSH