Limits...
Squid pen chitin chitooligomers as food colorants absorbers.

Liang TW, Huang CT, Dzung NA, Wang SL - Mar Drugs (2015)

Bottom Line: In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4).Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption.Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. QQ1987pp@hotmail.com.

ABSTRACT
One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

Show MeSH
Chemical structures of disperse dyes and food colorants. (a) disperse red 60; (b) disperse yellow 54; (c) Allura Red AC; (d) Tartrazne.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4306958&req=5

marinedrugs-13-00681-f001: Chemical structures of disperse dyes and food colorants. (a) disperse red 60; (b) disperse yellow 54; (c) Allura Red AC; (d) Tartrazne.

Mentions: A Bacillus cereus strain TKU033 that was capable of utilizing SPP to produce chitosanase was isolated from soil samples. The TKU033 chitosanase was purified, and its biochemical features were also characterized. In addition, the applications of the endo-type TKU033 chitosanase in functional chitooligomer production were also examined. To decrease the cost of adsorbents for dye removal, comparisons of the adsorption rates of the fermented SPP and the unfermented SPP for the disperse dyes (hydrophobic pigments, disperse red 60 (Figure 1a) and disperse yellow 54 (Figure 1b)) and water-soluble food colorants (Allura Red AC, R40 (Figure 1c); and Tartrazne, Y4 (Figure 1d)) were also undertaken.


Squid pen chitin chitooligomers as food colorants absorbers.

Liang TW, Huang CT, Dzung NA, Wang SL - Mar Drugs (2015)

Chemical structures of disperse dyes and food colorants. (a) disperse red 60; (b) disperse yellow 54; (c) Allura Red AC; (d) Tartrazne.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306958&req=5

marinedrugs-13-00681-f001: Chemical structures of disperse dyes and food colorants. (a) disperse red 60; (b) disperse yellow 54; (c) Allura Red AC; (d) Tartrazne.
Mentions: A Bacillus cereus strain TKU033 that was capable of utilizing SPP to produce chitosanase was isolated from soil samples. The TKU033 chitosanase was purified, and its biochemical features were also characterized. In addition, the applications of the endo-type TKU033 chitosanase in functional chitooligomer production were also examined. To decrease the cost of adsorbents for dye removal, comparisons of the adsorption rates of the fermented SPP and the unfermented SPP for the disperse dyes (hydrophobic pigments, disperse red 60 (Figure 1a) and disperse yellow 54 (Figure 1b)) and water-soluble food colorants (Allura Red AC, R40 (Figure 1c); and Tartrazne, Y4 (Figure 1d)) were also undertaken.

Bottom Line: In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4).Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption.Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

View Article: PubMed Central - PubMed

Affiliation: Life Science Development Center, Tamkang University, No. 151, Yingchuan Rd., Tamsui, New Taipei City 25137, Taiwan. QQ1987pp@hotmail.com.

ABSTRACT
One of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (COS). TKU033 chitosanase was induced from squid pen powder (SPP)-containing Bacillus cereus TKU033 medium and purified by ammonium sulfate precipitation and column chromatography. The enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °C in a pH 5 buffer solution for 60 min. Furthermore, the enzyme used for the COS preparation was also studied. The enzyme products revealed various mixtures of COS that with different degrees of polymerization (DP), ranging from three to nine. In the culture medium, the fermented SPP was recovered, and it displayed a better adsorption rate (up to 96%) for the disperse dyes than the water-soluble food colorants, Allura Red AC (R40) and Tartrazne (Y4). Fourier transform-infrared spectroscopic (FT-IR) analysis proved that the adsorption of the dyes onto fermented SPP was a physical adsorption. Results also showed that fermented SPP was a favorable adsorber and could be employed as low-cost alternative for dye removal in wastewater treatment.

Show MeSH