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Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on herpes simplex virus (HSV-1).

Kulshreshtha G, Burlot AS, Marty C, Critchley A, Hafting J, Bedoux G, Bourgougnon N, Prithiviraj B - Mar Drugs (2015)

Bottom Line: Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials.Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%-70% dry matter) as compared to aqueous extraction (20%-25% dry matter).Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. gr784654@dal.ca.

ABSTRACT
Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%-70% dry matter) as compared to aqueous extraction (20%-25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC50 of 77.6-126.8 μg/mL for CC and 36.5-41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID50/cells without cytotoxity (1-200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID50/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery.

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Total protein content in enzymatic hydrolysates of (a) Codium fragile (CF) and (b) Chondrus crispus (CC). Values with different superscript letters are significantly different (p < 0.05). Values represent mean ± standard deviation from three independent experiments (n = 9). P1: protease 1; C1: carbohydrases 1; C2: carbohydrases 2; C3: carbohydrases 3.
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marinedrugs-13-00558-f002: Total protein content in enzymatic hydrolysates of (a) Codium fragile (CF) and (b) Chondrus crispus (CC). Values with different superscript letters are significantly different (p < 0.05). Values represent mean ± standard deviation from three independent experiments (n = 9). P1: protease 1; C1: carbohydrases 1; C2: carbohydrases 2; C3: carbohydrases 3.

Mentions: The recovery of chemical fractions from the enzymatic hydrolysis of the two seaweeds is shown in Figure 2, Figure 3, Figure 4, Figure 5 and Figure 6. The percentage yield of each chemical compound in the hydrolysates is based on their dry weight percentage, obtained from the initial algal sample. Of all the chemical components, carbohydrases exhibited the highest percentage of dry matter in the hydrolysates.


Enzyme-assisted extraction of bioactive material from Chondrus crispus and Codium fragile and its effect on herpes simplex virus (HSV-1).

Kulshreshtha G, Burlot AS, Marty C, Critchley A, Hafting J, Bedoux G, Bourgougnon N, Prithiviraj B - Mar Drugs (2015)

Total protein content in enzymatic hydrolysates of (a) Codium fragile (CF) and (b) Chondrus crispus (CC). Values with different superscript letters are significantly different (p < 0.05). Values represent mean ± standard deviation from three independent experiments (n = 9). P1: protease 1; C1: carbohydrases 1; C2: carbohydrases 2; C3: carbohydrases 3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306952&req=5

marinedrugs-13-00558-f002: Total protein content in enzymatic hydrolysates of (a) Codium fragile (CF) and (b) Chondrus crispus (CC). Values with different superscript letters are significantly different (p < 0.05). Values represent mean ± standard deviation from three independent experiments (n = 9). P1: protease 1; C1: carbohydrases 1; C2: carbohydrases 2; C3: carbohydrases 3.
Mentions: The recovery of chemical fractions from the enzymatic hydrolysis of the two seaweeds is shown in Figure 2, Figure 3, Figure 4, Figure 5 and Figure 6. The percentage yield of each chemical compound in the hydrolysates is based on their dry weight percentage, obtained from the initial algal sample. Of all the chemical components, carbohydrases exhibited the highest percentage of dry matter in the hydrolysates.

Bottom Line: Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials.Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%-70% dry matter) as compared to aqueous extraction (20%-25% dry matter).Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Sciences, Faculty of Agriculture, Dalhousie University, PO Box 550, Truro, NS B2N 5E3, Canada. gr784654@dal.ca.

ABSTRACT
Codium fragile and Chondrus crispus are, respectively, green and red seaweeds which are abundant along the North Atlantic coasts. We investigated the chemical composition and antiviral activity of enzymatic extracts of C. fragile (CF) and C. crispus (CC). On a dry weight basis, CF consisted of 11% protein, 31% neutral sugars, 0.8% sulfate, 0.6% uronic acids, and 49% ash, while CC contained 27% protein, 28% neutral sugars, 17% sulfate, 1.8% uronic acids, and 25% ash. Enzyme-assisted hydrolysis improved the extraction efficiency of bioactive materials. Commercial proteases and carbohydrases significantly improved (p ≤ 0.001) biomass yield (40%-70% dry matter) as compared to aqueous extraction (20%-25% dry matter). Moreover, enzymatic hydrolysis enhanced the recovery of protein, neutral sugars, uronic acids, and sulfates. The enzymatic hydrolysates exhibited significant activity against Herpes simplex virus (HSV-1) with EC50 of 77.6-126.8 μg/mL for CC and 36.5-41.3 μg/mL for CF, at a multiplicity of infection (MOI) of 0.001 ID50/cells without cytotoxity (1-200 μg/mL). The extracts obtained from proteases (P1) and carbohydrases (C3) were also effective at higher virus MOI of 0.01 ID50/cells without cytotoxity. Taken together, these results indicate the potential application of enzymatic hydrolysates of C. fragile and C. crispus in functional food and antiviral drug discovery.

Show MeSH
Related in: MedlinePlus