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Does the use of chitosan contribute to oxalate kidney stone formation?

Fernandes Queiroz M, Melo KR, Sabry DA, Sassaki GL, Rocha HA - Mar Drugs (2014)

Bottom Line: However, intake of chitosan results in renal tissue accumulation of chitosan and promotes an increase in calcium excretion.On the other hand, the effect of chitosan on the formation of calcium oxalate crystals (CaOx) has not been described.However, it also showed excellent copper-chelating activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Biosciences Centre, Federal University of Rio Grande do Norte, Salgado Filho avenue 3000, Natal, RN 59078-970, Brazil. moacirfqn@gmail.com.

ABSTRACT
Chitosan is widely used in the biomedical field due its chemical and pharmacological properties. However, intake of chitosan results in renal tissue accumulation of chitosan and promotes an increase in calcium excretion. On the other hand, the effect of chitosan on the formation of calcium oxalate crystals (CaOx) has not been described. In this work, we evaluated the antioxidant capacity of chitosan and its interference in the formation of CaOx crystals in vitro. Here, the chitosan obtained commercially had its identity confirmed by nuclear magnetic resonance and infrared spectroscopy. In several tests, this chitosan showed low or no antioxidant activity. However, it also showed excellent copper-chelating activity. In vitro, chitosan acted as an inducer mainly of monohydrate CaOx crystal formation, which is more prevalent in patients with urolithiasis. We also observed that chitosan modifies the morphology and size of these crystals, as well as changes the surface charge of the crystals, making them even more positive, which can facilitate the interaction of these crystals with renal cells. Chitosan greatly influences the formation of crystals in vitro, and in vivo analyses should be conducted to assess the risk of using chitosan.

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FTIR spectrum of chitosan with the characteristic signs as evidence.
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marinedrugs-13-00141-f001: FTIR spectrum of chitosan with the characteristic signs as evidence.

Mentions: In Figure 1, we can observe the infrared spectrum of chitosan. A strong band in the region 3291–361 cm−1 corresponds to N-H and O-H stretching, as well as the intramolecular hydrogen bonds. The absorption bands at around 2921 and 2877 cm−1 can be attributed to C-H symmetric and asymmetric stretching, respectively. These bands are characteristics typical of polysaccharide and are found in other polysaccharide spectra, such as xylan [22], glucans [23] and carrageenans [24]. The presence of residual N-acetyl groups was confirmed by the bands at around 1645 cm−1 (C=O stretching of amide I) and 1325 cm−1 (C-N stretching of amide III), respectively. We did not find the small band at 1550 cm−1 that corresponds to N-H bending of amide II. This is the third band characteristic of typical N-acetyl groups, and it was probably overlapped by other bands. A band at 1589 cm−1 corresponds to the N-H bending of the primary amine [25]. The CH2 bending and CH3 symmetrical deformations were confirmed by the presence of bands at around 1423 and 1375 cm−1, respectively. The absorption band at 1153 cm−1 can be attributed to asymmetric stretching of the C-O-C bridge. The bands at 1066 and 1028 cm−1 correspond to C-O stretching. All bands are found in the spectra of samples of chitosan reported by others [26,27].


Does the use of chitosan contribute to oxalate kidney stone formation?

Fernandes Queiroz M, Melo KR, Sabry DA, Sassaki GL, Rocha HA - Mar Drugs (2014)

FTIR spectrum of chitosan with the characteristic signs as evidence.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306929&req=5

marinedrugs-13-00141-f001: FTIR spectrum of chitosan with the characteristic signs as evidence.
Mentions: In Figure 1, we can observe the infrared spectrum of chitosan. A strong band in the region 3291–361 cm−1 corresponds to N-H and O-H stretching, as well as the intramolecular hydrogen bonds. The absorption bands at around 2921 and 2877 cm−1 can be attributed to C-H symmetric and asymmetric stretching, respectively. These bands are characteristics typical of polysaccharide and are found in other polysaccharide spectra, such as xylan [22], glucans [23] and carrageenans [24]. The presence of residual N-acetyl groups was confirmed by the bands at around 1645 cm−1 (C=O stretching of amide I) and 1325 cm−1 (C-N stretching of amide III), respectively. We did not find the small band at 1550 cm−1 that corresponds to N-H bending of amide II. This is the third band characteristic of typical N-acetyl groups, and it was probably overlapped by other bands. A band at 1589 cm−1 corresponds to the N-H bending of the primary amine [25]. The CH2 bending and CH3 symmetrical deformations were confirmed by the presence of bands at around 1423 and 1375 cm−1, respectively. The absorption band at 1153 cm−1 can be attributed to asymmetric stretching of the C-O-C bridge. The bands at 1066 and 1028 cm−1 correspond to C-O stretching. All bands are found in the spectra of samples of chitosan reported by others [26,27].

Bottom Line: However, intake of chitosan results in renal tissue accumulation of chitosan and promotes an increase in calcium excretion.On the other hand, the effect of chitosan on the formation of calcium oxalate crystals (CaOx) has not been described.However, it also showed excellent copper-chelating activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Biosciences Centre, Federal University of Rio Grande do Norte, Salgado Filho avenue 3000, Natal, RN 59078-970, Brazil. moacirfqn@gmail.com.

ABSTRACT
Chitosan is widely used in the biomedical field due its chemical and pharmacological properties. However, intake of chitosan results in renal tissue accumulation of chitosan and promotes an increase in calcium excretion. On the other hand, the effect of chitosan on the formation of calcium oxalate crystals (CaOx) has not been described. In this work, we evaluated the antioxidant capacity of chitosan and its interference in the formation of CaOx crystals in vitro. Here, the chitosan obtained commercially had its identity confirmed by nuclear magnetic resonance and infrared spectroscopy. In several tests, this chitosan showed low or no antioxidant activity. However, it also showed excellent copper-chelating activity. In vitro, chitosan acted as an inducer mainly of monohydrate CaOx crystal formation, which is more prevalent in patients with urolithiasis. We also observed that chitosan modifies the morphology and size of these crystals, as well as changes the surface charge of the crystals, making them even more positive, which can facilitate the interaction of these crystals with renal cells. Chitosan greatly influences the formation of crystals in vitro, and in vivo analyses should be conducted to assess the risk of using chitosan.

Show MeSH
Related in: MedlinePlus