Limits...
Intestinal absorption of fucoidan extracted from the brown seaweed, Cladosiphon okamuranus.

Nagamine T, Nakazato K, Tomioka S, Iha M, Nakajima K - Mar Drugs (2014)

Bottom Line: It reached a maximum after 1 h and then rapidly decreased.Most fucoidan-positive cells also stained positive for ED1, suggesting that fucoidan was incorporated into intestinal macrophages.In conclusion, the absorption of fucoidan through the small intestine was demonstrated both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Health Sciences, Gunma University, Maebashi, Gunma 371-8514, Japan. mine@gunma-u.ac.jp.

ABSTRACT
The aim of this study was to examine the absorption of fucoidan through the intestinal tract. Fucoidan (0.1, 0.5, 1.0, 1.5 and 2.0 mg/mL) was added to Transwell inserts containing Caco-2 cells. The transport of fucoidan across Caco-2 cells increased in a dose-dependent manner up to 1.0 mg/mL. It reached a maximum after 1 h and then rapidly decreased. In another experiment, rats were fed standard chow containing 2% fucoidan for one or two weeks. Immunohistochemical staining revealed that fucoidan accumulated in jejunal epithelial cells, mononuclear cells in the jejunal lamina propria and sinusoidal non-parenchymal cells in the liver. Since we previously speculated that nitrosamine may enhance the intestinal absorption of fucoidan, its absorption was estimated in rats administered N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) in their drinking water. Rats were fed 0.2% fucoidan chow (BBN + 0.2% fucoidan rats), 2% fucoidan chow (BBN + 2% fucoidan rats) and standard chow for eight weeks. The uptake of fucoidan through the intestinal tract seemed to be low, but was measurable by our ELISA method. Fucoidan-positive cells were abundant in the small intestinal mucosa of BBN + 2% fucoidan rats. Most fucoidan-positive cells also stained positive for ED1, suggesting that fucoidan was incorporated into intestinal macrophages. The uptake of fucoidan by Kupffer cells was observed in the livers of BBN + 2% fucoidan rats. In conclusion, the absorption of fucoidan through the small intestine was demonstrated both in vivo and in vitro.

Show MeSH

Related in: MedlinePlus

Immunohistochemistry for fucoidan in the jejunum from one-week fucoidan rat (n = 2). (A) Fucoidan stained diffusely in the epithelial cells of the jejunum (×400). Enlargement of the red frame (C). (B) Some mononuclear cells stained positively for fucoidan in the lamina propria of the jejunum (×400). Enlargement of the red frame (D). Arrows indicate positive staining for fucoidan. Bars: 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4306924&req=5

marinedrugs-13-00048-f002: Immunohistochemistry for fucoidan in the jejunum from one-week fucoidan rat (n = 2). (A) Fucoidan stained diffusely in the epithelial cells of the jejunum (×400). Enlargement of the red frame (C). (B) Some mononuclear cells stained positively for fucoidan in the lamina propria of the jejunum (×400). Enlargement of the red frame (D). Arrows indicate positive staining for fucoidan. Bars: 50 μm.

Mentions: Immunohistochemistry for fucoidan showed that it was incorporated into some epithelial cells in the jejunum from rats fed 2% fucoidan for one week (one-week fucoidan rat), in which epithelial cells stained diffusely for fucoidan (Figure 2). Linear staining was also observed in the gap between adjacent epithelial cells. Fucoidan was detected in round mononuclear cells in the lamia propria of the jejunum; however, staining for fucoidan was very weak in the ileum.


Intestinal absorption of fucoidan extracted from the brown seaweed, Cladosiphon okamuranus.

Nagamine T, Nakazato K, Tomioka S, Iha M, Nakajima K - Mar Drugs (2014)

Immunohistochemistry for fucoidan in the jejunum from one-week fucoidan rat (n = 2). (A) Fucoidan stained diffusely in the epithelial cells of the jejunum (×400). Enlargement of the red frame (C). (B) Some mononuclear cells stained positively for fucoidan in the lamina propria of the jejunum (×400). Enlargement of the red frame (D). Arrows indicate positive staining for fucoidan. Bars: 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306924&req=5

marinedrugs-13-00048-f002: Immunohistochemistry for fucoidan in the jejunum from one-week fucoidan rat (n = 2). (A) Fucoidan stained diffusely in the epithelial cells of the jejunum (×400). Enlargement of the red frame (C). (B) Some mononuclear cells stained positively for fucoidan in the lamina propria of the jejunum (×400). Enlargement of the red frame (D). Arrows indicate positive staining for fucoidan. Bars: 50 μm.
Mentions: Immunohistochemistry for fucoidan showed that it was incorporated into some epithelial cells in the jejunum from rats fed 2% fucoidan for one week (one-week fucoidan rat), in which epithelial cells stained diffusely for fucoidan (Figure 2). Linear staining was also observed in the gap between adjacent epithelial cells. Fucoidan was detected in round mononuclear cells in the lamia propria of the jejunum; however, staining for fucoidan was very weak in the ileum.

Bottom Line: It reached a maximum after 1 h and then rapidly decreased.Most fucoidan-positive cells also stained positive for ED1, suggesting that fucoidan was incorporated into intestinal macrophages.In conclusion, the absorption of fucoidan through the small intestine was demonstrated both in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Health Sciences, Gunma University, Maebashi, Gunma 371-8514, Japan. mine@gunma-u.ac.jp.

ABSTRACT
The aim of this study was to examine the absorption of fucoidan through the intestinal tract. Fucoidan (0.1, 0.5, 1.0, 1.5 and 2.0 mg/mL) was added to Transwell inserts containing Caco-2 cells. The transport of fucoidan across Caco-2 cells increased in a dose-dependent manner up to 1.0 mg/mL. It reached a maximum after 1 h and then rapidly decreased. In another experiment, rats were fed standard chow containing 2% fucoidan for one or two weeks. Immunohistochemical staining revealed that fucoidan accumulated in jejunal epithelial cells, mononuclear cells in the jejunal lamina propria and sinusoidal non-parenchymal cells in the liver. Since we previously speculated that nitrosamine may enhance the intestinal absorption of fucoidan, its absorption was estimated in rats administered N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) in their drinking water. Rats were fed 0.2% fucoidan chow (BBN + 0.2% fucoidan rats), 2% fucoidan chow (BBN + 2% fucoidan rats) and standard chow for eight weeks. The uptake of fucoidan through the intestinal tract seemed to be low, but was measurable by our ELISA method. Fucoidan-positive cells were abundant in the small intestinal mucosa of BBN + 2% fucoidan rats. Most fucoidan-positive cells also stained positive for ED1, suggesting that fucoidan was incorporated into intestinal macrophages. The uptake of fucoidan by Kupffer cells was observed in the livers of BBN + 2% fucoidan rats. In conclusion, the absorption of fucoidan through the small intestine was demonstrated both in vivo and in vitro.

Show MeSH
Related in: MedlinePlus