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Sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells in mice.

Guo CY, Luo L, Urata Y, Goto S, Huang WJ, Takamura S, Hayashi F, Doi H, Kitajima Y, Ono Y, Ogi T, Li TS - Sci Rep (2015)

Bottom Line: Adult C57BL/6 mice were daily exposed to 0, 2, 10, 50, and 250 mGy γ-ray for 1 month in succession, respectively.Daily exposure to over 10 mGy γ-ray significantly decreased the number and colony-forming capacity of hematopoietic stem/progenitor cells at acute phase, and did not completely recover at chronic phase with 250 mGy exposure.Our data have clearly shown the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells of mice with daily exposures to 2 ~ 250 mGy γ-ray.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan [2] Department of Thoracic Surgery, Jiangxi Cancer Hospital, Nanchang, Jiangxi, PR China.

ABSTRACT
We evaluated the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells. Adult C57BL/6 mice were daily exposed to 0, 2, 10, 50, and 250 mGy γ-ray for 1 month in succession, respectively. The damage of hematopoietic stem/progenitor cells in bone marrow were investigated within 2 hours (acute phase) or at 3 months (chronic phase) after the last exposure. Daily exposure to over 10 mGy γ-ray significantly decreased the number and colony-forming capacity of hematopoietic stem/progenitor cells at acute phase, and did not completely recover at chronic phase with 250 mGy exposure. Interestingly, the daily exposure to 10 or 50 mGy γ-ray decreased the formation of mixed types of colonies at chronic phase, but the total number of colonies was comparable to control. Immunostaining analysis showed that the formation of 53BP1 foci in c-kit(+) stem/progenitor cells was significantly increased with daily exposure to 50 and 250 mGy at acute phase, and 250 mGy at chronic phase. Many genes involved in toxicity responses were up- or down-regulated with the exposures to all doses. Our data have clearly shown the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells of mice with daily exposures to 2 ~ 250 mGy γ-ray.

No MeSH data available.


Related in: MedlinePlus

Colony-forming assay.Bone marrow mononuclear cells were isolated from mice soon (A,C) or 3 months (B,D) after daily radiation exposures to 2, 10, 50, and 250 mGy γ-ray, and then cultured in methylcellulose complete medium. The colony formation was observed under microscopy at 9 days after incubation. The number of all types of colonies (>30 cells) and the mixed colonies (at least two types of cells in colony) were counted, and data was represent the mean of duplicate assays. * p<0.05 vs control; ** p<0.01 vs control.
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f3: Colony-forming assay.Bone marrow mononuclear cells were isolated from mice soon (A,C) or 3 months (B,D) after daily radiation exposures to 2, 10, 50, and 250 mGy γ-ray, and then cultured in methylcellulose complete medium. The colony formation was observed under microscopy at 9 days after incubation. The number of all types of colonies (>30 cells) and the mixed colonies (at least two types of cells in colony) were counted, and data was represent the mean of duplicate assays. * p<0.05 vs control; ** p<0.01 vs control.

Mentions: By using colony forming assay, we found that the total number of colonies grew from BM-MNCs was significantly decreased in the mice daily exposed to over 50 mGy γ-ray at acute phase (p < 0.01 vs. Control, Fig 3A), and also significantly decreased in the mice daily exposed to 250 mGy γ-ray at chronic phase (p < 0.01 vs. Control, Fig 3B). To further distinct the sensitivity of different types of stem/progenitor to radiation injury, we also counted the mixed colonies that grown from an early stage stem/progenitor cell with multiple differentiation potency. Interestingly, even if with a daily exposure to 10 mGy for 1 month, the formation of mixed colonies was significantly decreased (p < 0.01 vs. Control, Fig 3C), and this decrease did not completely recovered in 3 months after the completion of daily radiation exposures (p < 0.01 vs. Control, Fig 3D).


Sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells in mice.

Guo CY, Luo L, Urata Y, Goto S, Huang WJ, Takamura S, Hayashi F, Doi H, Kitajima Y, Ono Y, Ogi T, Li TS - Sci Rep (2015)

Colony-forming assay.Bone marrow mononuclear cells were isolated from mice soon (A,C) or 3 months (B,D) after daily radiation exposures to 2, 10, 50, and 250 mGy γ-ray, and then cultured in methylcellulose complete medium. The colony formation was observed under microscopy at 9 days after incubation. The number of all types of colonies (>30 cells) and the mixed colonies (at least two types of cells in colony) were counted, and data was represent the mean of duplicate assays. * p<0.05 vs control; ** p<0.01 vs control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306913&req=5

f3: Colony-forming assay.Bone marrow mononuclear cells were isolated from mice soon (A,C) or 3 months (B,D) after daily radiation exposures to 2, 10, 50, and 250 mGy γ-ray, and then cultured in methylcellulose complete medium. The colony formation was observed under microscopy at 9 days after incubation. The number of all types of colonies (>30 cells) and the mixed colonies (at least two types of cells in colony) were counted, and data was represent the mean of duplicate assays. * p<0.05 vs control; ** p<0.01 vs control.
Mentions: By using colony forming assay, we found that the total number of colonies grew from BM-MNCs was significantly decreased in the mice daily exposed to over 50 mGy γ-ray at acute phase (p < 0.01 vs. Control, Fig 3A), and also significantly decreased in the mice daily exposed to 250 mGy γ-ray at chronic phase (p < 0.01 vs. Control, Fig 3B). To further distinct the sensitivity of different types of stem/progenitor to radiation injury, we also counted the mixed colonies that grown from an early stage stem/progenitor cell with multiple differentiation potency. Interestingly, even if with a daily exposure to 10 mGy for 1 month, the formation of mixed colonies was significantly decreased (p < 0.01 vs. Control, Fig 3C), and this decrease did not completely recovered in 3 months after the completion of daily radiation exposures (p < 0.01 vs. Control, Fig 3D).

Bottom Line: Adult C57BL/6 mice were daily exposed to 0, 2, 10, 50, and 250 mGy γ-ray for 1 month in succession, respectively.Daily exposure to over 10 mGy γ-ray significantly decreased the number and colony-forming capacity of hematopoietic stem/progenitor cells at acute phase, and did not completely recover at chronic phase with 250 mGy exposure.Our data have clearly shown the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells of mice with daily exposures to 2 ~ 250 mGy γ-ray.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan [2] Department of Thoracic Surgery, Jiangxi Cancer Hospital, Nanchang, Jiangxi, PR China.

ABSTRACT
We evaluated the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells. Adult C57BL/6 mice were daily exposed to 0, 2, 10, 50, and 250 mGy γ-ray for 1 month in succession, respectively. The damage of hematopoietic stem/progenitor cells in bone marrow were investigated within 2 hours (acute phase) or at 3 months (chronic phase) after the last exposure. Daily exposure to over 10 mGy γ-ray significantly decreased the number and colony-forming capacity of hematopoietic stem/progenitor cells at acute phase, and did not completely recover at chronic phase with 250 mGy exposure. Interestingly, the daily exposure to 10 or 50 mGy γ-ray decreased the formation of mixed types of colonies at chronic phase, but the total number of colonies was comparable to control. Immunostaining analysis showed that the formation of 53BP1 foci in c-kit(+) stem/progenitor cells was significantly increased with daily exposure to 50 and 250 mGy at acute phase, and 250 mGy at chronic phase. Many genes involved in toxicity responses were up- or down-regulated with the exposures to all doses. Our data have clearly shown the sensitivity and dose dependency of radiation-induced injury in hematopoietic stem/progenitor cells of mice with daily exposures to 2 ~ 250 mGy γ-ray.

No MeSH data available.


Related in: MedlinePlus