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Polycyclic aromatic hydrocarbon residues in serum samples of autopsied individuals from Tennessee.

Ramesh A, Kumar A, Aramandla MP, Nyanda AM - Int J Environ Res Public Health (2014)

Bottom Line: The PAH residue levels detected were high in African Americans compared to Caucasians, Asians, and Hispanics.It appears that environmental exposure, dietary intake and in some cases occupational exposure may have contributed to the PAH body burden.While the PAH residue concentrations measured fall within the range of those reported for healthy adults elsewhere, in isolated cases, the concentrations detected were high, calling the need for a reduction in PAH emissions and human biomonitoring studies for purposes of risk assessment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry & Cancer Biology, Meharry Medical College, 1005 D.B. Todd Blvd., Nashville, TN 37208, USA. aramesh@mmc.edu.

ABSTRACT
This study reports the concentrations of polycyclic aromatic hydrocarbons (PAHs) in human blood sera samples (n = 650) obtained at autopsy from individuals who died of drug abuse, alcohol toxicity, homicide, suicide and other unknown causes. The analyzed samples from decedents revealed the presence of PAHs of which B(a)P was the most predominant one, followed by benzo(b)fluoranthene and benzo(k)fluoranthene. The other PAHs detected sporadically and measured were benzo(g,h,i)perylene, acenaphthene, anthracene, phenanthrene, and fluoranthene The mean concentrations of PAHs were greater in the twenties to fifties age groups compared to others. The PAH residue levels detected were high in African Americans compared to Caucasians, Asians, and Hispanics. It appears that environmental exposure, dietary intake and in some cases occupational exposure may have contributed to the PAH body burden. While the PAH residue concentrations measured fall within the range of those reported for healthy adults elsewhere, in isolated cases, the concentrations detected were high, calling the need for a reduction in PAH emissions and human biomonitoring studies for purposes of risk assessment.

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Related in: MedlinePlus

Chromatogram showing peaks of benzoylecgonine (metabolite standard for cocaine) and trimipramine (a tricyclic antidepressant used as internal standard). Since PAHs were detected during screening of autopsy samples for drug abuse, this chromatogram is shown to present the retention time for the cocaine metabolite.
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ijerph-12-00322-f001: Chromatogram showing peaks of benzoylecgonine (metabolite standard for cocaine) and trimipramine (a tricyclic antidepressant used as internal standard). Since PAHs were detected during screening of autopsy samples for drug abuse, this chromatogram is shown to present the retention time for the cocaine metabolite.

Mentions: Given that B(a)P and/or other PAHs undergo biotransformation, producing an array of metabolites, questions might arise whether the B(a)P peaks identified from the mass spectrometer include the parent compound as well as the metabolites. In several instances, we have come across multiple peaks that were identified as B(a)P by the mass spectral library. The mass spectral data for B(a)P shows a base peak [8]. The other peaks represent metabolites of B(a)P with different retention times [9] that were resolved by the mass spectrometer. Therefore, for data reporting purposes, the concentrations of all B(a)P derivatives detected and measured during a single run were pooled together to report as the sum total of B(a)P concentrations measured for each sample. Representative chromatograms showing the analytes are presented in Figure 1, Figure 2 and Figure 3. The chromatograms showed a B(a)P peak with a retention time of 31.65 min. The putative B(a)P metabolites showed peaks with retention times of 30.16, 30.29, and 30.30 min. The B(b)f parent compound showed a peak with retention time of 29.84 min, while one of its metabolites showed a peak at 29.11 min. Similarly, the B(k)f parent compound showed a peak with retention time of 31.98 min, while one of its metabolites showed a peak at 31.67 min. Since the objective of our study was not detecting and quantifying individual PAH metabolites, no metabolite standards were used to identify the key metabolites of PAHs. Additionally, since the samples are from autopsy cases whether metabolite stability may have been compromised is not known.


Polycyclic aromatic hydrocarbon residues in serum samples of autopsied individuals from Tennessee.

Ramesh A, Kumar A, Aramandla MP, Nyanda AM - Int J Environ Res Public Health (2014)

Chromatogram showing peaks of benzoylecgonine (metabolite standard for cocaine) and trimipramine (a tricyclic antidepressant used as internal standard). Since PAHs were detected during screening of autopsy samples for drug abuse, this chromatogram is shown to present the retention time for the cocaine metabolite.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306864&req=5

ijerph-12-00322-f001: Chromatogram showing peaks of benzoylecgonine (metabolite standard for cocaine) and trimipramine (a tricyclic antidepressant used as internal standard). Since PAHs were detected during screening of autopsy samples for drug abuse, this chromatogram is shown to present the retention time for the cocaine metabolite.
Mentions: Given that B(a)P and/or other PAHs undergo biotransformation, producing an array of metabolites, questions might arise whether the B(a)P peaks identified from the mass spectrometer include the parent compound as well as the metabolites. In several instances, we have come across multiple peaks that were identified as B(a)P by the mass spectral library. The mass spectral data for B(a)P shows a base peak [8]. The other peaks represent metabolites of B(a)P with different retention times [9] that were resolved by the mass spectrometer. Therefore, for data reporting purposes, the concentrations of all B(a)P derivatives detected and measured during a single run were pooled together to report as the sum total of B(a)P concentrations measured for each sample. Representative chromatograms showing the analytes are presented in Figure 1, Figure 2 and Figure 3. The chromatograms showed a B(a)P peak with a retention time of 31.65 min. The putative B(a)P metabolites showed peaks with retention times of 30.16, 30.29, and 30.30 min. The B(b)f parent compound showed a peak with retention time of 29.84 min, while one of its metabolites showed a peak at 29.11 min. Similarly, the B(k)f parent compound showed a peak with retention time of 31.98 min, while one of its metabolites showed a peak at 31.67 min. Since the objective of our study was not detecting and quantifying individual PAH metabolites, no metabolite standards were used to identify the key metabolites of PAHs. Additionally, since the samples are from autopsy cases whether metabolite stability may have been compromised is not known.

Bottom Line: The PAH residue levels detected were high in African Americans compared to Caucasians, Asians, and Hispanics.It appears that environmental exposure, dietary intake and in some cases occupational exposure may have contributed to the PAH body burden.While the PAH residue concentrations measured fall within the range of those reported for healthy adults elsewhere, in isolated cases, the concentrations detected were high, calling the need for a reduction in PAH emissions and human biomonitoring studies for purposes of risk assessment.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry & Cancer Biology, Meharry Medical College, 1005 D.B. Todd Blvd., Nashville, TN 37208, USA. aramesh@mmc.edu.

ABSTRACT
This study reports the concentrations of polycyclic aromatic hydrocarbons (PAHs) in human blood sera samples (n = 650) obtained at autopsy from individuals who died of drug abuse, alcohol toxicity, homicide, suicide and other unknown causes. The analyzed samples from decedents revealed the presence of PAHs of which B(a)P was the most predominant one, followed by benzo(b)fluoranthene and benzo(k)fluoranthene. The other PAHs detected sporadically and measured were benzo(g,h,i)perylene, acenaphthene, anthracene, phenanthrene, and fluoranthene The mean concentrations of PAHs were greater in the twenties to fifties age groups compared to others. The PAH residue levels detected were high in African Americans compared to Caucasians, Asians, and Hispanics. It appears that environmental exposure, dietary intake and in some cases occupational exposure may have contributed to the PAH body burden. While the PAH residue concentrations measured fall within the range of those reported for healthy adults elsewhere, in isolated cases, the concentrations detected were high, calling the need for a reduction in PAH emissions and human biomonitoring studies for purposes of risk assessment.

Show MeSH
Related in: MedlinePlus