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Protective effects of pine bark extract against cisplatin-induced hepatotoxicity and oxidative stress in rats.

Ko JW, Lee IC, Park SH, Moon C, Kang SS, Kim SH, Kim JC - Lab Anim Res (2014)

Bottom Line: In addition, an increase in the malondialdehyde (MDA) concentration and a decrease in the reduced glutathione (GSH) content and catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) activities were observed in the cisplatin-treated rat hepatic tissues.Moreover, PYC treatment also induced antioxidant activity by decreasing MDA level and increasing GSH content and SOD and GST activities in liver tissues.These results indicate that PYC has a protective effect against acute hepatotoxicity induced by cisplatin in rats, and that the protective effects of PYC may be due to inhibiting lipid peroxidation and increasing antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.

ABSTRACT
We investigated the protective effects of pine bark extract (pycnogenol®, PYC) against cisplatin-induced hepatotoxicity and oxidative stress in rats. Twenty-four male rats were divided into the following four groups: (1) vehicle control, (2) cisplatin (7.5 mg/kg), (3) cisplatin & PYC 10 (10 mg/kg/day), and (4) cisplatin & PYC 20 (20 mg/kg/day). A single intraperitoneal injection of cisplatin induced hepatotoxicity, as evidenced by an increase in serum aminotransferase and histopathological alterations, including degeneration/necrosis of hepatocytes, vacuolation, and sinusoidal dilation. In addition, an increase in the malondialdehyde (MDA) concentration and a decrease in the reduced glutathione (GSH) content and catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) activities were observed in the cisplatin-treated rat hepatic tissues. In contrast, PYC treatment effectively prevented cisplatin-induced hepatotoxicity, including the elevation of aminotransferase and histopathological lesions, in a dosedependent manner. Moreover, PYC treatment also induced antioxidant activity by decreasing MDA level and increasing GSH content and SOD and GST activities in liver tissues. These results indicate that PYC has a protective effect against acute hepatotoxicity induced by cisplatin in rats, and that the protective effects of PYC may be due to inhibiting lipid peroxidation and increasing antioxidant activity.

No MeSH data available.


Related in: MedlinePlus

(A) Malondialdehyde (MDA) and (B) reduced glutathione (GSH) concentrations in the liver of male rats treated with cisplatin and/or PYC. Each bar represents the mean±SD (n=6). **P<0.01 versus the control group; ††P<0.01 versus the cisplatin group.
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Figure 2: (A) Malondialdehyde (MDA) and (B) reduced glutathione (GSH) concentrations in the liver of male rats treated with cisplatin and/or PYC. Each bar represents the mean±SD (n=6). **P<0.01 versus the control group; ††P<0.01 versus the cisplatin group.

Mentions: The MDA concentration and GSH activity in liver tissues are presented in Figure 2. The concentration of MDA, an end product of LPO, in rats treated with cisplatin increased significantly, whereas hepatic GSH content decreased significantly compared to those in the control group. However, hepatic MDA concentration decreased significantly in a dose-dependent manner in the cisplatin & PYC groups, compared to that in the cisplatin group. Hepatic GSH content also dose-dependently increased compared to that in the cisplatin group.


Protective effects of pine bark extract against cisplatin-induced hepatotoxicity and oxidative stress in rats.

Ko JW, Lee IC, Park SH, Moon C, Kang SS, Kim SH, Kim JC - Lab Anim Res (2014)

(A) Malondialdehyde (MDA) and (B) reduced glutathione (GSH) concentrations in the liver of male rats treated with cisplatin and/or PYC. Each bar represents the mean±SD (n=6). **P<0.01 versus the control group; ††P<0.01 versus the cisplatin group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306705&req=5

Figure 2: (A) Malondialdehyde (MDA) and (B) reduced glutathione (GSH) concentrations in the liver of male rats treated with cisplatin and/or PYC. Each bar represents the mean±SD (n=6). **P<0.01 versus the control group; ††P<0.01 versus the cisplatin group.
Mentions: The MDA concentration and GSH activity in liver tissues are presented in Figure 2. The concentration of MDA, an end product of LPO, in rats treated with cisplatin increased significantly, whereas hepatic GSH content decreased significantly compared to those in the control group. However, hepatic MDA concentration decreased significantly in a dose-dependent manner in the cisplatin & PYC groups, compared to that in the cisplatin group. Hepatic GSH content also dose-dependently increased compared to that in the cisplatin group.

Bottom Line: In addition, an increase in the malondialdehyde (MDA) concentration and a decrease in the reduced glutathione (GSH) content and catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) activities were observed in the cisplatin-treated rat hepatic tissues.Moreover, PYC treatment also induced antioxidant activity by decreasing MDA level and increasing GSH content and SOD and GST activities in liver tissues.These results indicate that PYC has a protective effect against acute hepatotoxicity induced by cisplatin in rats, and that the protective effects of PYC may be due to inhibiting lipid peroxidation and increasing antioxidant activity.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.

ABSTRACT
We investigated the protective effects of pine bark extract (pycnogenol®, PYC) against cisplatin-induced hepatotoxicity and oxidative stress in rats. Twenty-four male rats were divided into the following four groups: (1) vehicle control, (2) cisplatin (7.5 mg/kg), (3) cisplatin & PYC 10 (10 mg/kg/day), and (4) cisplatin & PYC 20 (20 mg/kg/day). A single intraperitoneal injection of cisplatin induced hepatotoxicity, as evidenced by an increase in serum aminotransferase and histopathological alterations, including degeneration/necrosis of hepatocytes, vacuolation, and sinusoidal dilation. In addition, an increase in the malondialdehyde (MDA) concentration and a decrease in the reduced glutathione (GSH) content and catalase (CAT), superoxide dismutase (SOD), and glutathione S-transferase (GST) activities were observed in the cisplatin-treated rat hepatic tissues. In contrast, PYC treatment effectively prevented cisplatin-induced hepatotoxicity, including the elevation of aminotransferase and histopathological lesions, in a dosedependent manner. Moreover, PYC treatment also induced antioxidant activity by decreasing MDA level and increasing GSH content and SOD and GST activities in liver tissues. These results indicate that PYC has a protective effect against acute hepatotoxicity induced by cisplatin in rats, and that the protective effects of PYC may be due to inhibiting lipid peroxidation and increasing antioxidant activity.

No MeSH data available.


Related in: MedlinePlus