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Effects of pectin lyase-modified red ginseng extracts in high-fat diet-fed obese mice.

Lee HY, Park KH, Park YM, Moon DI, Oh HG, Kwon DY, Yang HJ, Kim O, Kim DW, Yoo JH, Hong SC, Lee KH, Seol SY, Park YS, Park JD, Pyo MK - Lab Anim Res (2014)

Bottom Line: Treatment of GS-E3D reduced differentiation of 3T3-L1 adipocytes with low cytotoxicity.In the animal model, compared to the high fat diet control, serum glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol, TG, and leptin level were reduced in treatment animals in a dose-dependent manner.In addition, we found that GS-E3D could decrease total hepatic lipid droplets.

View Article: PubMed Central - PubMed

Affiliation: Huvet Co., Ltd., Iksan, Korea.

ABSTRACT
Red ginseng and its extracts have been used as traditional medicines and functional foods in countries worldwide. The aim of this study was to examine the bioavailability of pectin lyase-modified red ginseng extracts (GS-E3D), and the effects of GS-E3D on adipogenesis of 3T3-L1 adipocytes, as well as on metabolic disorders such as hyperglycemia, dyslipidemia, and fatty liver in high-fat diet fed obese C57BL/6 mice. Mice were divided into 5 groups: normal diet group, high fat diet-vehicle group, high fat diet + 0.1 g/kg GS-E3D (0.1-GS-E3D), high fat diet + 0.3 g/kg (0.3-GS-E3D), high fat diet + 1.0 g/kg (1.0-GS-E3D). Treatment of GS-E3D reduced differentiation of 3T3-L1 adipocytes with low cytotoxicity. In the animal model, compared to the high fat diet control, serum glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol, TG, and leptin level were reduced in treatment animals in a dose-dependent manner. In addition, we found that GS-E3D could decrease total hepatic lipid droplets. These results suggest that GS-E3D, as a dietary supplement, has beneficial effects on obesity and may have useful effects in health-care products.

No MeSH data available.


Related in: MedlinePlus

Effects of GS-E3D on histological hepatic steatosis in a mouse obesity model induced by a high-fat diet. (A) Normal group, (B) high fat diet-vehicle group, (C) high fat diet+GS-E3D 0.1 g/kg, (D) high fat diet+GS-E3D 0.3 g/kg, (E) high fat diet+GS-E3D 1.0 g/kg, (F) steatosis grade score. (400× magnification). Scale bar=100 µµm. a,b,c,dValues in the row with different superscript letters are significantly different; P<0.05. Data are shown as mean±SE (n=8).
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Figure 11: Effects of GS-E3D on histological hepatic steatosis in a mouse obesity model induced by a high-fat diet. (A) Normal group, (B) high fat diet-vehicle group, (C) high fat diet+GS-E3D 0.1 g/kg, (D) high fat diet+GS-E3D 0.3 g/kg, (E) high fat diet+GS-E3D 1.0 g/kg, (F) steatosis grade score. (400× magnification). Scale bar=100 µµm. a,b,c,dValues in the row with different superscript letters are significantly different; P<0.05. Data are shown as mean±SE (n=8).

Mentions: To determine ameliorative effects of GS-E3D on high fat diet-induced hepatic steatosis, we observed lipid droplet contents through an H&E stain in liver after 4 weeks (Figure 11). High fat diet mice had significantly increased steatosis scores, but administration of F-BS reduced the number of lipid droplets in a dose-dependent manner (Figure 11F).


Effects of pectin lyase-modified red ginseng extracts in high-fat diet-fed obese mice.

Lee HY, Park KH, Park YM, Moon DI, Oh HG, Kwon DY, Yang HJ, Kim O, Kim DW, Yoo JH, Hong SC, Lee KH, Seol SY, Park YS, Park JD, Pyo MK - Lab Anim Res (2014)

Effects of GS-E3D on histological hepatic steatosis in a mouse obesity model induced by a high-fat diet. (A) Normal group, (B) high fat diet-vehicle group, (C) high fat diet+GS-E3D 0.1 g/kg, (D) high fat diet+GS-E3D 0.3 g/kg, (E) high fat diet+GS-E3D 1.0 g/kg, (F) steatosis grade score. (400× magnification). Scale bar=100 µµm. a,b,c,dValues in the row with different superscript letters are significantly different; P<0.05. Data are shown as mean±SE (n=8).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4306702&req=5

Figure 11: Effects of GS-E3D on histological hepatic steatosis in a mouse obesity model induced by a high-fat diet. (A) Normal group, (B) high fat diet-vehicle group, (C) high fat diet+GS-E3D 0.1 g/kg, (D) high fat diet+GS-E3D 0.3 g/kg, (E) high fat diet+GS-E3D 1.0 g/kg, (F) steatosis grade score. (400× magnification). Scale bar=100 µµm. a,b,c,dValues in the row with different superscript letters are significantly different; P<0.05. Data are shown as mean±SE (n=8).
Mentions: To determine ameliorative effects of GS-E3D on high fat diet-induced hepatic steatosis, we observed lipid droplet contents through an H&E stain in liver after 4 weeks (Figure 11). High fat diet mice had significantly increased steatosis scores, but administration of F-BS reduced the number of lipid droplets in a dose-dependent manner (Figure 11F).

Bottom Line: Treatment of GS-E3D reduced differentiation of 3T3-L1 adipocytes with low cytotoxicity.In the animal model, compared to the high fat diet control, serum glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol, TG, and leptin level were reduced in treatment animals in a dose-dependent manner.In addition, we found that GS-E3D could decrease total hepatic lipid droplets.

View Article: PubMed Central - PubMed

Affiliation: Huvet Co., Ltd., Iksan, Korea.

ABSTRACT
Red ginseng and its extracts have been used as traditional medicines and functional foods in countries worldwide. The aim of this study was to examine the bioavailability of pectin lyase-modified red ginseng extracts (GS-E3D), and the effects of GS-E3D on adipogenesis of 3T3-L1 adipocytes, as well as on metabolic disorders such as hyperglycemia, dyslipidemia, and fatty liver in high-fat diet fed obese C57BL/6 mice. Mice were divided into 5 groups: normal diet group, high fat diet-vehicle group, high fat diet + 0.1 g/kg GS-E3D (0.1-GS-E3D), high fat diet + 0.3 g/kg (0.3-GS-E3D), high fat diet + 1.0 g/kg (1.0-GS-E3D). Treatment of GS-E3D reduced differentiation of 3T3-L1 adipocytes with low cytotoxicity. In the animal model, compared to the high fat diet control, serum glucose, total cholesterol, LDL-cholesterol, HDL-cholesterol, TG, and leptin level were reduced in treatment animals in a dose-dependent manner. In addition, we found that GS-E3D could decrease total hepatic lipid droplets. These results suggest that GS-E3D, as a dietary supplement, has beneficial effects on obesity and may have useful effects in health-care products.

No MeSH data available.


Related in: MedlinePlus