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Relative roles of ABCG5/ABCG8 in liver and intestine.

Wang J, Mitsche MA, Lütjohann D, Cohen JC, Xie XS, Hobbs HH - J. Lipid Res. (2014)

Bottom Line: ABCG5 (G5) and ABCG8 (G8) form a sterol transporter that acts in liver and intestine to prevent accumulation of dietary sterols.Hepatic G5G8 mediates cholesterol excretion into bile, but the function and relative importance of intestinal G5G8 has not been defined.Expression of G5G8 only in intestine or only in liver decreased tissue sterol levels by 90% when compared with G5G8(-/-) animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390.

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Expression of G5 and G8 mRNA (A) and protein (B) in the liver and intestine of WT, liver-specific (L-G5G8−/−), intestine-specific (I-G5G8−/−), and total G5G8−/− KO mice. A: RT-PCR was performed to quantitate levels of G5 and G8 mRNA in mice of the designated genotypes (five female mice/group, 12–15 weeks old). Cyclophilin was used as an internal control for mRNA expression. The mean values of the WT mice were set to 1. Standardized means ± SEM are shown. B: Immunoblot analysis of G5 and G8 in membranes isolated from the liver and intestine of mice was performed as described in the Materials and Methods. M, mature; P, precursor. The asterisk indicates a nonspecific band.
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fig1: Expression of G5 and G8 mRNA (A) and protein (B) in the liver and intestine of WT, liver-specific (L-G5G8−/−), intestine-specific (I-G5G8−/−), and total G5G8−/− KO mice. A: RT-PCR was performed to quantitate levels of G5 and G8 mRNA in mice of the designated genotypes (five female mice/group, 12–15 weeks old). Cyclophilin was used as an internal control for mRNA expression. The mean values of the WT mice were set to 1. Standardized means ± SEM are shown. B: Immunoblot analysis of G5 and G8 in membranes isolated from the liver and intestine of mice was performed as described in the Materials and Methods. M, mature; P, precursor. The asterisk indicates a nonspecific band.

Mentions: To ensure that both G5 and G8 were inactivated in a tissue-specific manner, we measured levels of G5 and G8 mRNA (Fig. 1A) and protein (Fig. 1B) in livers and intestines of the mice. No G5 or G8 mRNA or protein was detected in the livers of the L-G5G8−/− mice or in the intestine of I-G5G8−/− mice. Selective inactivation of G5G8 in one tissue did not alter the level of expression in the other tissue. The levels of G5G8 mRNA and protein in the intestines of the L-G5G8−/− mice and in the livers of the I-G5G8−/− were similar to the levels in WT animals.


Relative roles of ABCG5/ABCG8 in liver and intestine.

Wang J, Mitsche MA, Lütjohann D, Cohen JC, Xie XS, Hobbs HH - J. Lipid Res. (2014)

Expression of G5 and G8 mRNA (A) and protein (B) in the liver and intestine of WT, liver-specific (L-G5G8−/−), intestine-specific (I-G5G8−/−), and total G5G8−/− KO mice. A: RT-PCR was performed to quantitate levels of G5 and G8 mRNA in mice of the designated genotypes (five female mice/group, 12–15 weeks old). Cyclophilin was used as an internal control for mRNA expression. The mean values of the WT mice were set to 1. Standardized means ± SEM are shown. B: Immunoblot analysis of G5 and G8 in membranes isolated from the liver and intestine of mice was performed as described in the Materials and Methods. M, mature; P, precursor. The asterisk indicates a nonspecific band.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4306686&req=5

fig1: Expression of G5 and G8 mRNA (A) and protein (B) in the liver and intestine of WT, liver-specific (L-G5G8−/−), intestine-specific (I-G5G8−/−), and total G5G8−/− KO mice. A: RT-PCR was performed to quantitate levels of G5 and G8 mRNA in mice of the designated genotypes (five female mice/group, 12–15 weeks old). Cyclophilin was used as an internal control for mRNA expression. The mean values of the WT mice were set to 1. Standardized means ± SEM are shown. B: Immunoblot analysis of G5 and G8 in membranes isolated from the liver and intestine of mice was performed as described in the Materials and Methods. M, mature; P, precursor. The asterisk indicates a nonspecific band.
Mentions: To ensure that both G5 and G8 were inactivated in a tissue-specific manner, we measured levels of G5 and G8 mRNA (Fig. 1A) and protein (Fig. 1B) in livers and intestines of the mice. No G5 or G8 mRNA or protein was detected in the livers of the L-G5G8−/− mice or in the intestine of I-G5G8−/− mice. Selective inactivation of G5G8 in one tissue did not alter the level of expression in the other tissue. The levels of G5G8 mRNA and protein in the intestines of the L-G5G8−/− mice and in the livers of the I-G5G8−/− were similar to the levels in WT animals.

Bottom Line: ABCG5 (G5) and ABCG8 (G8) form a sterol transporter that acts in liver and intestine to prevent accumulation of dietary sterols.Hepatic G5G8 mediates cholesterol excretion into bile, but the function and relative importance of intestinal G5G8 has not been defined.Expression of G5G8 only in intestine or only in liver decreased tissue sterol levels by 90% when compared with G5G8(-/-) animals.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390.

Show MeSH
Related in: MedlinePlus