Relative roles of ABCG5/ABCG8 in liver and intestine.
Bottom Line: ABCG5 (G5) and ABCG8 (G8) form a sterol transporter that acts in liver and intestine to prevent accumulation of dietary sterols.Hepatic G5G8 mediates cholesterol excretion into bile, but the function and relative importance of intestinal G5G8 has not been defined.Expression of G5G8 only in intestine or only in liver decreased tissue sterol levels by 90% when compared with G5G8(-/-) animals.
Affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390.Show MeSH
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Mentions: To ensure that both G5 and G8 were inactivated in a tissue-specific manner, we measured levels of G5 and G8 mRNA (Fig. 1A) and protein (Fig. 1B) in livers and intestines of the mice. No G5 or G8 mRNA or protein was detected in the livers of the L-G5G8−/− mice or in the intestine of I-G5G8−/− mice. Selective inactivation of G5G8 in one tissue did not alter the level of expression in the other tissue. The levels of G5G8 mRNA and protein in the intestines of the L-G5G8−/− mice and in the livers of the I-G5G8−/− were similar to the levels in WT animals.
Affiliation: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390.