Limits...
Intestinal Peyer's patches prevent tumorigenesis in Apc (Min/+) mice.

Fujimoto K, Fujii G, Sakurai H, Yoshitome H, Mutoh M, Wada M - J Clin Biochem Nutr (2014)

Bottom Line: Using this model, we increased the number of Peyer's patches using 0.1% and 1% corn husk arabinoxylan through feed.Immune reaction following corn husk arabinoxylan treatment was measured by cytokine array.Increasing the number of Peyer's patches decreased interleukin-17 production, which showed a dose dependent correlation with transcription factor/lymphoid enhancer-binding factor.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Biology, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo-shi, Nagasaki 859-3298, Japan.

ABSTRACT
Peyer's patches are nodules that play a central role in intestinal immunity. Few studies demonstrate the relationship between the number of Peyer's patches and intestinal polyps. Here we identify a statistically significant inverse correlation between the quantity of Peyer's patches and of the development of intestinal polyps in Apc (Min/+) mice, which are a useful model to clarify the role of Peyer's patches in intestinal tumorigenesis. Using this model, we increased the number of Peyer's patches using 0.1% and 1% corn husk arabinoxylan through feed. Intestinal polyp formation significantly decreased, concomitant with an increase in Peyer's patches development (n = 12/group). In Aly (-/-) Apc (Min/+) mice (negative control; no Peyer's patches) there was no change in the amount of intestinal polyps (n = 10/group). Immune reaction following corn husk arabinoxylan treatment was measured by cytokine array. Increasing the number of Peyer's patches decreased interleukin-17 production, which showed a dose dependent correlation with transcription factor/lymphoid enhancer-binding factor. This study identified a relationship between levels of Peyer's patches and intestinal polyp formation, partly explained by the involvement of interleukin-17 production and β-catenin signaling in Apc (Min/+) mice.

No MeSH data available.


Related in: MedlinePlus

TCF/LEF transcriptional activity was induced by IL-17 in Caco-2 cells. The cells were stimulated with 5 mM sodium butyrate. Data are representative of at least there independent experiments. The TCF/LEF transcriptional activity was significantly increased with 0.5 ng/ml of IL-17 (*1 = p<0.001, Dunnett’s multiple comparison test).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4306656&req=5

Figure 6: TCF/LEF transcriptional activity was induced by IL-17 in Caco-2 cells. The cells were stimulated with 5 mM sodium butyrate. Data are representative of at least there independent experiments. The TCF/LEF transcriptional activity was significantly increased with 0.5 ng/ml of IL-17 (*1 = p<0.001, Dunnett’s multiple comparison test).

Mentions: We examined TCF/LEF-dependent transcriptional activity after 24-h treatment with IL-17 in Caco-2 cell transfected with pTOP-Flash or pFOP-Flash and SV40-RenillaLuc, and differentiated with 5 mM sodium butyrate (Fig. 6). Caco-2 cells are an established cell line derived from colorectal tumors with loss of APC gene function.


Intestinal Peyer's patches prevent tumorigenesis in Apc (Min/+) mice.

Fujimoto K, Fujii G, Sakurai H, Yoshitome H, Mutoh M, Wada M - J Clin Biochem Nutr (2014)

TCF/LEF transcriptional activity was induced by IL-17 in Caco-2 cells. The cells were stimulated with 5 mM sodium butyrate. Data are representative of at least there independent experiments. The TCF/LEF transcriptional activity was significantly increased with 0.5 ng/ml of IL-17 (*1 = p<0.001, Dunnett’s multiple comparison test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4306656&req=5

Figure 6: TCF/LEF transcriptional activity was induced by IL-17 in Caco-2 cells. The cells were stimulated with 5 mM sodium butyrate. Data are representative of at least there independent experiments. The TCF/LEF transcriptional activity was significantly increased with 0.5 ng/ml of IL-17 (*1 = p<0.001, Dunnett’s multiple comparison test).
Mentions: We examined TCF/LEF-dependent transcriptional activity after 24-h treatment with IL-17 in Caco-2 cell transfected with pTOP-Flash or pFOP-Flash and SV40-RenillaLuc, and differentiated with 5 mM sodium butyrate (Fig. 6). Caco-2 cells are an established cell line derived from colorectal tumors with loss of APC gene function.

Bottom Line: Using this model, we increased the number of Peyer's patches using 0.1% and 1% corn husk arabinoxylan through feed.Immune reaction following corn husk arabinoxylan treatment was measured by cytokine array.Increasing the number of Peyer's patches decreased interleukin-17 production, which showed a dose dependent correlation with transcription factor/lymphoid enhancer-binding factor.

View Article: PubMed Central - PubMed

Affiliation: Division of Molecular Biology, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo-shi, Nagasaki 859-3298, Japan.

ABSTRACT
Peyer's patches are nodules that play a central role in intestinal immunity. Few studies demonstrate the relationship between the number of Peyer's patches and intestinal polyps. Here we identify a statistically significant inverse correlation between the quantity of Peyer's patches and of the development of intestinal polyps in Apc (Min/+) mice, which are a useful model to clarify the role of Peyer's patches in intestinal tumorigenesis. Using this model, we increased the number of Peyer's patches using 0.1% and 1% corn husk arabinoxylan through feed. Intestinal polyp formation significantly decreased, concomitant with an increase in Peyer's patches development (n = 12/group). In Aly (-/-) Apc (Min/+) mice (negative control; no Peyer's patches) there was no change in the amount of intestinal polyps (n = 10/group). Immune reaction following corn husk arabinoxylan treatment was measured by cytokine array. Increasing the number of Peyer's patches decreased interleukin-17 production, which showed a dose dependent correlation with transcription factor/lymphoid enhancer-binding factor. This study identified a relationship between levels of Peyer's patches and intestinal polyp formation, partly explained by the involvement of interleukin-17 production and β-catenin signaling in Apc (Min/+) mice.

No MeSH data available.


Related in: MedlinePlus